Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies.
We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are know...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2018
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0006396 https://doaj.org/article/0bfdaa85b1974bdabed5dc11a98e8ef2 |
id |
ftdoajarticles:oai:doaj.org/article:0bfdaa85b1974bdabed5dc11a98e8ef2 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0bfdaa85b1974bdabed5dc11a98e8ef2 2023-05-15T15:06:31+02:00 Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. Sascha Knauf Simone Lüert David Šmajs Michal Strouhal Idrissa S Chuma Sieghard Frischmann Mohammed Bakheit 2018-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006396 https://doaj.org/article/0bfdaa85b1974bdabed5dc11a98e8ef2 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5978989?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006396 https://doaj.org/article/0bfdaa85b1974bdabed5dc11a98e8ef2 PLoS Neglected Tropical Diseases, Vol 12, Iss 4, p e0006396 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006396 2022-12-31T12:35:59Z We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 4 e0006396 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sascha Knauf Simone Lüert David Šmajs Michal Strouhal Idrissa S Chuma Sieghard Frischmann Mohammed Bakheit Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
We show proof of concept for gene targets (polA, tprL, and TP_0619) that can be used in loop-mediated isothermal amplification (LAMP) assays to rapidly differentiate infection with any of the three Treponema pallidum subspecies (pallidum (TPA), pertenue (TPE), and endemicum (TEN)) and which are known to infect humans and nonhuman primates (NHPs). Four TPA, six human, and two NHP TPE strains, as well as two human TEN strains were used to establish and validate the LAMP assays. All three LAMP assays were highly specific for the target DNA. Amplification was rapid (5-15 min) and within a range of 10E+6 to 10E+2 of target DNA molecules. Performance in NHP clinical samples was similar to the one seen in human TPE strains. The newly designed LAMP assays provide proof of concept for a diagnostic tool that enhances yaws clinical diagnosis. It is highly specific for the target DNA and does not require expensive laboratory equipment. Test results can potentially be interpreted with the naked eye, which makes it suitable for the use in remote clinical settings. |
format |
Article in Journal/Newspaper |
author |
Sascha Knauf Simone Lüert David Šmajs Michal Strouhal Idrissa S Chuma Sieghard Frischmann Mohammed Bakheit |
author_facet |
Sascha Knauf Simone Lüert David Šmajs Michal Strouhal Idrissa S Chuma Sieghard Frischmann Mohammed Bakheit |
author_sort |
Sascha Knauf |
title |
Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. |
title_short |
Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. |
title_full |
Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. |
title_fullStr |
Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. |
title_full_unstemmed |
Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies. |
title_sort |
gene target selection for loop-mediated isothermal amplification for rapid discrimination of treponema pallidum subspecies. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006396 https://doaj.org/article/0bfdaa85b1974bdabed5dc11a98e8ef2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 4, p e0006396 (2018) |
op_relation |
http://europepmc.org/articles/PMC5978989?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006396 https://doaj.org/article/0bfdaa85b1974bdabed5dc11a98e8ef2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006396 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
4 |
container_start_page |
e0006396 |
_version_ |
1766338114560720896 |