Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica
Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using na...
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ftdoajarticles:oai:doaj.org/article:0bee1f5daac84103bd4e3ef186a943f4 2024-01-07T09:40:03+01:00 Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica Xiao-Jie Liu Jie Zhang Peng-Hui Hong Zheng-Jun Li 2016-08-01T00:00:00Z https://doi.org/10.7717/peerj.2291 https://doaj.org/article/0bee1f5daac84103bd4e3ef186a943f4 EN eng PeerJ Inc. https://peerj.com/articles/2291.pdf https://peerj.com/articles/2291/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.2291 2167-8359 https://doaj.org/article/0bee1f5daac84103bd4e3ef186a943f4 PeerJ, Vol 4, p e2291 (2016) Halophilic Neptunomonas antarctica Poly-3-hydroxybutyrate Seawater Marine bacteria Medicine R Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.7717/peerj.2291 2023-12-10T01:50:36Z Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using natural seawater as medium components, PHB was produced up to 2.12 g/L with a yield of 0.18 g PHB/g fructose. In the presence of artificial seawater, the PHB titer and yield reached 2.13 g/L and 0.13 g PHB/g fructose, respectively. The accumulated polymer gradually decreased when fructose was exhausted, indicating that intracellular PHB was degraded by N. antarctica. The weight-average and number-average molecular weights of PHB produced within natural seawater were 2.4 × 105 g/mol and 1.7 × 105 g/mol, respectively. Our results highlight the potential of N. antarctica for PHB production with seawater as a nutrient source. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles PeerJ 4 e2291 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Halophilic Neptunomonas antarctica Poly-3-hydroxybutyrate Seawater Marine bacteria Medicine R Biology (General) QH301-705.5 |
spellingShingle |
Halophilic Neptunomonas antarctica Poly-3-hydroxybutyrate Seawater Marine bacteria Medicine R Biology (General) QH301-705.5 Xiao-Jie Liu Jie Zhang Peng-Hui Hong Zheng-Jun Li Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
topic_facet |
Halophilic Neptunomonas antarctica Poly-3-hydroxybutyrate Seawater Marine bacteria Medicine R Biology (General) QH301-705.5 |
description |
Considering the industrial interest of biodegradable polymer poly-3-hydroxybutyrate (PHB), the marine bacteria Neptunomonas antarctica was studied for its ability to accumulate PHB. The extracted polymer was confirmed to be PHB by nuclear magnetic resonance analysis. In shake flask cultures using natural seawater as medium components, PHB was produced up to 2.12 g/L with a yield of 0.18 g PHB/g fructose. In the presence of artificial seawater, the PHB titer and yield reached 2.13 g/L and 0.13 g PHB/g fructose, respectively. The accumulated polymer gradually decreased when fructose was exhausted, indicating that intracellular PHB was degraded by N. antarctica. The weight-average and number-average molecular weights of PHB produced within natural seawater were 2.4 × 105 g/mol and 1.7 × 105 g/mol, respectively. Our results highlight the potential of N. antarctica for PHB production with seawater as a nutrient source. |
format |
Article in Journal/Newspaper |
author |
Xiao-Jie Liu Jie Zhang Peng-Hui Hong Zheng-Jun Li |
author_facet |
Xiao-Jie Liu Jie Zhang Peng-Hui Hong Zheng-Jun Li |
author_sort |
Xiao-Jie Liu |
title |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_short |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_full |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_fullStr |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_full_unstemmed |
Microbial production and characterization of poly-3-hydroxybutyrate by Neptunomonas antarctica |
title_sort |
microbial production and characterization of poly-3-hydroxybutyrate by neptunomonas antarctica |
publisher |
PeerJ Inc. |
publishDate |
2016 |
url |
https://doi.org/10.7717/peerj.2291 https://doaj.org/article/0bee1f5daac84103bd4e3ef186a943f4 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
PeerJ, Vol 4, p e2291 (2016) |
op_relation |
https://peerj.com/articles/2291.pdf https://peerj.com/articles/2291/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.2291 2167-8359 https://doaj.org/article/0bee1f5daac84103bd4e3ef186a943f4 |
op_doi |
https://doi.org/10.7717/peerj.2291 |
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PeerJ |
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4 |
container_start_page |
e2291 |
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1787430415119679488 |