A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth
Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:0b46840f4abb4369a4132960dfce6452 2023-05-15T15:08:04+02:00 A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth Islamudin Ahmad Neneng Siti Silfi Ambarwati Berna Elya Hanita Omar Kamarza Mulia Arry Yanuar Osamu Negishi Abdul Mun´im 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.260398 https://doaj.org/article/0b46840f4abb4369a4132960dfce6452 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=6;spage=257;epage=262;aulast=Ahmad https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.260398 https://doaj.org/article/0b46840f4abb4369a4132960dfce6452 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 6, Pp 257-262 (2019) 2 3 5 - t r i m e t h o x y - 9 - ( 1 2 1 4 1 5 - trimethoxybenzyl)-1h-indene angiotensin-converting enzyme inhibitor pellucidin a peperomia pellucida (l) kunth Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.260398 2022-12-31T02:19:33Z Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively. Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 6 257 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
2 3 5 - t r i m e t h o x y - 9 - ( 1 2 1 4 1 5 - trimethoxybenzyl)-1h-indene angiotensin-converting enzyme inhibitor pellucidin a peperomia pellucida (l) kunth Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
2 3 5 - t r i m e t h o x y - 9 - ( 1 2 1 4 1 5 - trimethoxybenzyl)-1h-indene angiotensin-converting enzyme inhibitor pellucidin a peperomia pellucida (l) kunth Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Islamudin Ahmad Neneng Siti Silfi Ambarwati Berna Elya Hanita Omar Kamarza Mulia Arry Yanuar Osamu Negishi Abdul Mun´im A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
topic_facet |
2 3 5 - t r i m e t h o x y - 9 - ( 1 2 1 4 1 5 - trimethoxybenzyl)-1h-indene angiotensin-converting enzyme inhibitor pellucidin a peperomia pellucida (l) kunth Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To isolate, identify, and evaluate a new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth herbs. Methods: A dried sample of Peperomia pellucida herb was successively macerated with n-hexane and ethyl acetate. The ethyl acetate extract solution was evaporated to obtain the crude extract. Vacuum liquid column chromatography and thin layer chromatography were performed to obtain two pure compounds. Then, both compounds were elucidated and identified using the spectroscopic method. Angiotensin-converting enzyme inhibitory activity studies of both compounds were determined using angiotensin-converting enzyme kit WST-1 with spectrophotometer microplate reader 96-well at 450 nm wavelength. Results: Two bioactive compounds were successfully isolated from Peperomia pellucida herb, including a new compound of 2,3,5-trimethoxy-9-(12,14,15-trimethoxybenzyl)-1H-indene and pellucidin A. Both compounds demonstrated angiotensin-converting enzyme inhibitory activity, with IC50 values of 72 μM (27.95 μg/mL) and 11 μM (4.4 μg/mL), respectively. Conclusions: In the present study, two active angiotensin-converting enzyme inhibitors were successfully isolated and purified from Peperomia pellucida which is used as an antihypertensive in traditional medicine, and support its use as an angiotensin-converting enzyme-inhibiting drug. |
format |
Article in Journal/Newspaper |
author |
Islamudin Ahmad Neneng Siti Silfi Ambarwati Berna Elya Hanita Omar Kamarza Mulia Arry Yanuar Osamu Negishi Abdul Mun´im |
author_facet |
Islamudin Ahmad Neneng Siti Silfi Ambarwati Berna Elya Hanita Omar Kamarza Mulia Arry Yanuar Osamu Negishi Abdul Mun´im |
author_sort |
Islamudin Ahmad |
title |
A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
title_short |
A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
title_full |
A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
title_fullStr |
A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
title_full_unstemmed |
A new angiotensin-converting enzyme inhibitor from Peperomia pellucida (L.) Kunth |
title_sort |
new angiotensin-converting enzyme inhibitor from peperomia pellucida (l.) kunth |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.260398 https://doaj.org/article/0b46840f4abb4369a4132960dfce6452 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 6, Pp 257-262 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=6;spage=257;epage=262;aulast=Ahmad https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.260398 https://doaj.org/article/0b46840f4abb4369a4132960dfce6452 |
op_doi |
https://doi.org/10.4103/2221-1691.260398 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
6 |
container_start_page |
257 |
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1766339496945647616 |