Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action
Objective: To evaluate the essential oils (EO) composition, antimicrobial and antioxidant power of a local plant, Daucus gracilis (D. gracilis). Methods: The aerial parts of D. gracilis were subjected to hydro distillation by a Clevenger apparatus type to obtain the EO which had been analyzed by gas...
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Wolters Kluwer Medknow Publications
2016
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ftdoajarticles:oai:doaj.org/article:0b2147f3524646019c0d878a64b17fce 2023-05-15T15:14:52+02:00 Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action Meriem El Kolli Hocine Laouer Hayet El Kolli Salah Akkal Farida Sahli 2016-01-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.08.004 https://doaj.org/article/0b2147f3524646019c0d878a64b17fce EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115002506 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.08.004 https://doaj.org/article/0b2147f3524646019c0d878a64b17fce Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 1, Pp 8-15 (2016) Daucus gracilis Apiaceae Essential oils Antimicrobial activity Macro-dilution Micro-dilution Anti-oxidative activity Reducing power Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.08.004 2023-01-08T01:33:57Z Objective: To evaluate the essential oils (EO) composition, antimicrobial and antioxidant power of a local plant, Daucus gracilis (D. gracilis). Methods: The aerial parts of D. gracilis were subjected to hydro distillation by a Clevenger apparatus type to obtain the EO which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry, and screened for antimicrobial activity against five bacteria and three fungi by agar diffusion method. The mechanism of action of the EO was determined on the susceptible strains by both of time kill assay and lysis experience. The minimal inhibitory concentrations were determined by agar macro-dilution and micro-dilution methods. Anti-oxidative properties of the EO were also studied by free diphenyl-2-picrylhydrazyl radical scavenging and reducing power techniques. Results: The EO yielded 0.68 (v/w). The chemical analysis presented two dominant constituents which were the elemicin (35.3%) and the geranyl acetate (26.8%). D. gracilis EO inhibited the growth of Bacillus cereus and Proteus mirabilis significantly with minimal inhibitory concentrations of 17.15 μg/mL by the agar dilution method and 57.05 μg/mL and 114.1 μg/mL, respectively by liquid micro-dilution. A remarkable decrease in a survival rate as well as in the absorbance in 260 nm was recorded, which suggested that the cytoplasm membrane was one of the targets of the EO. The EO showed, also, important anti-oxidative effects with an IC50 of 0.002 mg/mL and a dose-dependent reducing power. Conclusions: D. gracilis EO showed potent antimicrobial and anti-oxidative activities and had acted on the cytoplasm membrane. These activities could be exploited in the food industry for food preservation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 1 8 15 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Daucus gracilis Apiaceae Essential oils Antimicrobial activity Macro-dilution Micro-dilution Anti-oxidative activity Reducing power Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Daucus gracilis Apiaceae Essential oils Antimicrobial activity Macro-dilution Micro-dilution Anti-oxidative activity Reducing power Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Meriem El Kolli Hocine Laouer Hayet El Kolli Salah Akkal Farida Sahli Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action |
topic_facet |
Daucus gracilis Apiaceae Essential oils Antimicrobial activity Macro-dilution Micro-dilution Anti-oxidative activity Reducing power Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the essential oils (EO) composition, antimicrobial and antioxidant power of a local plant, Daucus gracilis (D. gracilis). Methods: The aerial parts of D. gracilis were subjected to hydro distillation by a Clevenger apparatus type to obtain the EO which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry, and screened for antimicrobial activity against five bacteria and three fungi by agar diffusion method. The mechanism of action of the EO was determined on the susceptible strains by both of time kill assay and lysis experience. The minimal inhibitory concentrations were determined by agar macro-dilution and micro-dilution methods. Anti-oxidative properties of the EO were also studied by free diphenyl-2-picrylhydrazyl radical scavenging and reducing power techniques. Results: The EO yielded 0.68 (v/w). The chemical analysis presented two dominant constituents which were the elemicin (35.3%) and the geranyl acetate (26.8%). D. gracilis EO inhibited the growth of Bacillus cereus and Proteus mirabilis significantly with minimal inhibitory concentrations of 17.15 μg/mL by the agar dilution method and 57.05 μg/mL and 114.1 μg/mL, respectively by liquid micro-dilution. A remarkable decrease in a survival rate as well as in the absorbance in 260 nm was recorded, which suggested that the cytoplasm membrane was one of the targets of the EO. The EO showed, also, important anti-oxidative effects with an IC50 of 0.002 mg/mL and a dose-dependent reducing power. Conclusions: D. gracilis EO showed potent antimicrobial and anti-oxidative activities and had acted on the cytoplasm membrane. These activities could be exploited in the food industry for food preservation. |
format |
Article in Journal/Newspaper |
author |
Meriem El Kolli Hocine Laouer Hayet El Kolli Salah Akkal Farida Sahli |
author_facet |
Meriem El Kolli Hocine Laouer Hayet El Kolli Salah Akkal Farida Sahli |
author_sort |
Meriem El Kolli |
title |
Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action |
title_short |
Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action |
title_full |
Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action |
title_fullStr |
Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action |
title_full_unstemmed |
Chemical analysis, antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action |
title_sort |
chemical analysis, antimicrobial and anti-oxidative properties of daucus gracilis essential oil and its mechanism of action |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2015.08.004 https://doaj.org/article/0b2147f3524646019c0d878a64b17fce |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 1, Pp 8-15 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115002506 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.08.004 https://doaj.org/article/0b2147f3524646019c0d878a64b17fce |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.08.004 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
1 |
container_start_page |
8 |
op_container_end_page |
15 |
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1766345264843456512 |