Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata
Objective: To evaluate the antimicrobial properties of Kappaphycus alvarezii (K. alvarezii) and Andrographis paniculata (A. paniculata) and to compare the microbial inhibition activities between these two crude extracts. Methods: Both K. alvarezii and A. paniculata were extracted with methanol befor...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.apjtb.2017.07.003 https://doaj.org/article/0b03001a55804b78a938b03fd52f0170 |
id |
ftdoajarticles:oai:doaj.org/article:0b03001a55804b78a938b03fd52f0170 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0b03001a55804b78a938b03fd52f0170 2023-05-15T15:11:52+02:00 Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata Xin Qi Chuah Wenlynn Mun Swee Sen Teo 2017-08-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.07.003 https://doaj.org/article/0b03001a55804b78a938b03fd52f0170 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117307578 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.07.003 https://doaj.org/article/0b03001a55804b78a938b03fd52f0170 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 8, Pp 729-731 (2017) Kappaphycus alvarezii Andrographis paniculata Anti-microbial Gram-positive Gram-negative Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.07.003 2022-12-30T21:51:51Z Objective: To evaluate the antimicrobial properties of Kappaphycus alvarezii (K. alvarezii) and Andrographis paniculata (A. paniculata) and to compare the microbial inhibition activities between these two crude extracts. Methods: Both K. alvarezii and A. paniculata were extracted with methanol before the commencement of antimicrobial properties studies. There were a total of eight species of bacteria, including Gram-positive bacteria Bacillus cereus, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis and Gram-negative bacteria, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella enterica. The antimicrobial activity was tested by disk diffusion method. Results: Crude extract of K. alvarezii was found not effective against both Gram-positive and Gram-negative bacteria. In contrast, A. paniculata showed higher inhibition towards the growth of Gram-positive bacteria compared to Gram-negative bacteria. Results revealed that Bacillus subtilis was susceptible at lower concentration of A. paniculata crude extract however Staphylococcus epidermidis was the most susceptible towards A. paniculata at higher concentration. Although the inhibition zones produced by the crude extract were smaller than that of the positive control, streptomycin disc, A. paniculata crude extract still can be considered as potential antimicrobial agents either because it is a natural product or the active compound which is yet identified from its crude extract. Conclusions: Crude extract of K. alvarezii has zero inhibition in bacteria growth whereas A. paniculata exerted higher inhibition towards Gram-positive bacteria. The bioactive compounds contained by A. paniculata can be evaluated in order to yield a better vision towards the mode of action. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 8 729 731 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Kappaphycus alvarezii Andrographis paniculata Anti-microbial Gram-positive Gram-negative Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Kappaphycus alvarezii Andrographis paniculata Anti-microbial Gram-positive Gram-negative Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Xin Qi Chuah Wenlynn Mun Swee Sen Teo Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata |
topic_facet |
Kappaphycus alvarezii Andrographis paniculata Anti-microbial Gram-positive Gram-negative Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the antimicrobial properties of Kappaphycus alvarezii (K. alvarezii) and Andrographis paniculata (A. paniculata) and to compare the microbial inhibition activities between these two crude extracts. Methods: Both K. alvarezii and A. paniculata were extracted with methanol before the commencement of antimicrobial properties studies. There were a total of eight species of bacteria, including Gram-positive bacteria Bacillus cereus, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis and Gram-negative bacteria, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella enterica. The antimicrobial activity was tested by disk diffusion method. Results: Crude extract of K. alvarezii was found not effective against both Gram-positive and Gram-negative bacteria. In contrast, A. paniculata showed higher inhibition towards the growth of Gram-positive bacteria compared to Gram-negative bacteria. Results revealed that Bacillus subtilis was susceptible at lower concentration of A. paniculata crude extract however Staphylococcus epidermidis was the most susceptible towards A. paniculata at higher concentration. Although the inhibition zones produced by the crude extract were smaller than that of the positive control, streptomycin disc, A. paniculata crude extract still can be considered as potential antimicrobial agents either because it is a natural product or the active compound which is yet identified from its crude extract. Conclusions: Crude extract of K. alvarezii has zero inhibition in bacteria growth whereas A. paniculata exerted higher inhibition towards Gram-positive bacteria. The bioactive compounds contained by A. paniculata can be evaluated in order to yield a better vision towards the mode of action. |
format |
Article in Journal/Newspaper |
author |
Xin Qi Chuah Wenlynn Mun Swee Sen Teo |
author_facet |
Xin Qi Chuah Wenlynn Mun Swee Sen Teo |
author_sort |
Xin Qi Chuah |
title |
Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata |
title_short |
Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata |
title_full |
Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata |
title_fullStr |
Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata |
title_full_unstemmed |
Comparison study of anti-microbial activity between crude extract of Kappaphycus alvarezii and Andrographis paniculata |
title_sort |
comparison study of anti-microbial activity between crude extract of kappaphycus alvarezii and andrographis paniculata |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.07.003 https://doaj.org/article/0b03001a55804b78a938b03fd52f0170 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 8, Pp 729-731 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117307578 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.07.003 https://doaj.org/article/0b03001a55804b78a938b03fd52f0170 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.07.003 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
8 |
container_start_page |
729 |
op_container_end_page |
731 |
_version_ |
1766342661431623680 |