The recently identified flavivirus Bamaga virus is transmitted horizontally by Culex mosquitoes and interferes with West Nile virus replication in vitro and transmission in vivo.
Arthropod-borne flaviviruses such as yellow fever (YFV), Zika and dengue viruses continue to cause significant human disease globally. These viruses are transmitted by mosquitoes when a female imbibes an infected blood-meal from a viremic vertebrate host and expectorates the virus into a subsequent...
Published in: | PLOS Neglected Tropical Diseases |
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2018
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Online Access: | https://doi.org/10.1371/journal.pntd.0006886 https://doaj.org/article/0b00c39c7723477da7d1dad3b87d4eb6 |
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ftdoajarticles:oai:doaj.org/article:0b00c39c7723477da7d1dad3b87d4eb6 2023-05-15T15:15:06+02:00 The recently identified flavivirus Bamaga virus is transmitted horizontally by Culex mosquitoes and interferes with West Nile virus replication in vitro and transmission in vivo. Agathe M G Colmant Sonja Hall-Mendelin Scott A Ritchie Helle Bielefeldt-Ohmann Jessica J Harrison Natalee D Newton Caitlin A O'Brien Chris Cazier Cheryl A Johansen Jody Hobson-Peters Roy A Hall Andrew F van den Hurk 2018-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006886 https://doaj.org/article/0b00c39c7723477da7d1dad3b87d4eb6 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6200184?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006886 https://doaj.org/article/0b00c39c7723477da7d1dad3b87d4eb6 PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006886 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006886 2022-12-31T15:19:02Z Arthropod-borne flaviviruses such as yellow fever (YFV), Zika and dengue viruses continue to cause significant human disease globally. These viruses are transmitted by mosquitoes when a female imbibes an infected blood-meal from a viremic vertebrate host and expectorates the virus into a subsequent host. Bamaga virus (BgV) is a flavivirus recently discovered in Culex sitiens subgroup mosquitoes collected from Cape York Peninsula, Australia. This virus phylogenetically clusters with the YFV group, but is potentially restricted in most vertebrates. However, high levels of replication in an opossum cell line (OK) indicate a potential association with marsupials. To ascertain whether BgV could be horizontally transmitted by mosquitoes, the vector competence of two members of the Cx. sitiens subgroup, Cx. annulirostris and Cx. sitiens, for BgV was investigated. Eleven to thirteen days after imbibing an infectious blood-meal, infection rates were 11.3% and 18.8% for Cx. annulirostris and Cx. sitiens, respectively. Cx. annulirostris transmitted the virus at low levels (5.6% had BgV-positive saliva overall); Cx. sitiens did not transmit the virus. When mosquitoes were injected intrathoracially with BgV, the infection and transmission rates were 100% and 82%, respectively, for both species. These results provided evidence for the first time that BgV can be transmitted horizontally by Cx. annulirostris, the primary vector of pathogenic zoonotic flaviviruses in Australia. We also assessed whether BgV could interfere with replication in vitro, and infection and transmission in vivo of super-infecting pathogenic Culex-associated flaviviruses. BgV significantly reduced growth of Murray Valley encephalitis and West Nile (WNV) viruses in vitro. While prior infection with BgV by injection did not inhibit WNV super-infection of Cx. annulirostris, significantly fewer BgV-infected mosquitoes could transmit WNV than mock-injected mosquitoes. Overall, these data contribute to our understanding of flavivirus ecology, modes of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Cape York ENVELOPE(-87.000,-87.000,73.801,73.801) PLOS Neglected Tropical Diseases 12 10 e0006886 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Agathe M G Colmant Sonja Hall-Mendelin Scott A Ritchie Helle Bielefeldt-Ohmann Jessica J Harrison Natalee D Newton Caitlin A O'Brien Chris Cazier Cheryl A Johansen Jody Hobson-Peters Roy A Hall Andrew F van den Hurk The recently identified flavivirus Bamaga virus is transmitted horizontally by Culex mosquitoes and interferes with West Nile virus replication in vitro and transmission in vivo. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Arthropod-borne flaviviruses such as yellow fever (YFV), Zika and dengue viruses continue to cause significant human disease globally. These viruses are transmitted by mosquitoes when a female imbibes an infected blood-meal from a viremic vertebrate host and expectorates the virus into a subsequent host. Bamaga virus (BgV) is a flavivirus recently discovered in Culex sitiens subgroup mosquitoes collected from Cape York Peninsula, Australia. This virus phylogenetically clusters with the YFV group, but is potentially restricted in most vertebrates. However, high levels of replication in an opossum cell line (OK) indicate a potential association with marsupials. To ascertain whether BgV could be horizontally transmitted by mosquitoes, the vector competence of two members of the Cx. sitiens subgroup, Cx. annulirostris and Cx. sitiens, for BgV was investigated. Eleven to thirteen days after imbibing an infectious blood-meal, infection rates were 11.3% and 18.8% for Cx. annulirostris and Cx. sitiens, respectively. Cx. annulirostris transmitted the virus at low levels (5.6% had BgV-positive saliva overall); Cx. sitiens did not transmit the virus. When mosquitoes were injected intrathoracially with BgV, the infection and transmission rates were 100% and 82%, respectively, for both species. These results provided evidence for the first time that BgV can be transmitted horizontally by Cx. annulirostris, the primary vector of pathogenic zoonotic flaviviruses in Australia. We also assessed whether BgV could interfere with replication in vitro, and infection and transmission in vivo of super-infecting pathogenic Culex-associated flaviviruses. BgV significantly reduced growth of Murray Valley encephalitis and West Nile (WNV) viruses in vitro. While prior infection with BgV by injection did not inhibit WNV super-infection of Cx. annulirostris, significantly fewer BgV-infected mosquitoes could transmit WNV than mock-injected mosquitoes. Overall, these data contribute to our understanding of flavivirus ecology, modes of ... |
format |
Article in Journal/Newspaper |
author |
Agathe M G Colmant Sonja Hall-Mendelin Scott A Ritchie Helle Bielefeldt-Ohmann Jessica J Harrison Natalee D Newton Caitlin A O'Brien Chris Cazier Cheryl A Johansen Jody Hobson-Peters Roy A Hall Andrew F van den Hurk |
author_facet |
Agathe M G Colmant Sonja Hall-Mendelin Scott A Ritchie Helle Bielefeldt-Ohmann Jessica J Harrison Natalee D Newton Caitlin A O'Brien Chris Cazier Cheryl A Johansen Jody Hobson-Peters Roy A Hall Andrew F van den Hurk |
author_sort |
Agathe M G Colmant |
title |
The recently identified flavivirus Bamaga virus is transmitted horizontally by Culex mosquitoes and interferes with West Nile virus replication in vitro and transmission in vivo. |
title_short |
The recently identified flavivirus Bamaga virus is transmitted horizontally by Culex mosquitoes and interferes with West Nile virus replication in vitro and transmission in vivo. |
title_full |
The recently identified flavivirus Bamaga virus is transmitted horizontally by Culex mosquitoes and interferes with West Nile virus replication in vitro and transmission in vivo. |
title_fullStr |
The recently identified flavivirus Bamaga virus is transmitted horizontally by Culex mosquitoes and interferes with West Nile virus replication in vitro and transmission in vivo. |
title_full_unstemmed |
The recently identified flavivirus Bamaga virus is transmitted horizontally by Culex mosquitoes and interferes with West Nile virus replication in vitro and transmission in vivo. |
title_sort |
recently identified flavivirus bamaga virus is transmitted horizontally by culex mosquitoes and interferes with west nile virus replication in vitro and transmission in vivo. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006886 https://doaj.org/article/0b00c39c7723477da7d1dad3b87d4eb6 |
long_lat |
ENVELOPE(-87.000,-87.000,73.801,73.801) |
geographic |
Arctic Cape York |
geographic_facet |
Arctic Cape York |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 10, p e0006886 (2018) |
op_relation |
http://europepmc.org/articles/PMC6200184?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006886 https://doaj.org/article/0b00c39c7723477da7d1dad3b87d4eb6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006886 |
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PLOS Neglected Tropical Diseases |
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12 |
container_issue |
10 |
container_start_page |
e0006886 |
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