The influence of CD 4+t cells, hiv disease stage and zidovudine on hiv isolation in Bahia, Brazil
HIV-l isolation was attempted on 72 individuais, including persons with knoum HIV infection and five without proven HIV infection but with indeterminate Western blot patterns, as well as on low-risk HIV seronegative persons. The ahility to detect HIV- 1 frorn culture supernatant by p24 antigen captu...
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Sociedade Brasileira de Medicina Tropical (SBMT)
1996
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ftdoajarticles:oai:doaj.org/article:0af90bfaae8144de8157899b6772b0aa 2023-05-15T15:16:16+02:00 The influence of CD 4+t cells, hiv disease stage and zidovudine on hiv isolation in Bahia, Brazil Carlos Brites Célia Pedroso Nanci Silva Warren D Johnson Jr Roberto Badaró 1996-02-01T00:00:00Z https://doaj.org/article/0af90bfaae8144de8157899b6772b0aa EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821996000100002 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/0af90bfaae8144de8157899b6772b0aa Revista da Sociedade Brasileira de Medicina Tropical, Vol 29, Iss 1, Pp 5-9 (1996) VIH p24 Isolamento viral Brasil CD4+ linfócitos T HIV Viral isolation Brazil CD4+ Tlymphocytes Arctic medicine. Tropical medicine RC955-962 article 1996 ftdoajarticles 2022-12-30T23:18:01Z HIV-l isolation was attempted on 72 individuais, including persons with knoum HIV infection and five without proven HIV infection but with indeterminate Western blot patterns, as well as on low-risk HIV seronegative persons. The ahility to detect HIV- 1 frorn culture supernatant by p24 antigen capture assay was evaluated by segregating patients by absolute CD4+ cell counts, clinicai stage of disease, p24 antigenemia and zidovudine use. The likelihood of a p24 positive HIV culture was highest among patients with CD4+ T-cell counts below 200/ul and patients with advanced clinical disease. Use of zidovudine did not affect the rate ofHIV positwity in cultures. Tentativa de isolamento do vírus tipo 1 da imunodeficiência adquirida (VIH-1) foi realizada em 72 indivíduos sendo 51 pacientes com sorologia positiva para o VIH-1, confirmada por Western blot; 5 doadores de sangue com padrão indeterminado ao Western blot; 3 indivíduos com diagnóstico clínico de AIDS, porém com sorologia negativa, e 13 profissionais de saúde soronegativos. Os pacientes foram estratificados de acordo com a contagem de células CD4+, estágio clínico , antigenemia (p24) e uso de zidovudine. As culturas para o VIH-1 foram positivas em 45/50 (90%) tentativas. Houve uma correlação inversa entre o número de células CD4+ e a freqüência de isolamento do VIH-1. As culturas foram positivas em 84% dos indivíduos com CD4+ <200, contra 48% d positividade naqueles com contagem de célula CD4+ acima deste valor. O uso de zidovudine não interferiu na positividade das culturas. Concluímo. que a sensibilidade dos métodos de culture qualitativo e quantitativo é similar para a detecção do VIH-1. A taxa de positividade das culturas não foi afetada pelo uso prévio de zidovudine, mas foi diretamente proporcional ao grau de imunodeficiência dos pacientes. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
VIH p24 Isolamento viral Brasil CD4+ linfócitos T HIV Viral isolation Brazil CD4+ Tlymphocytes Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
VIH p24 Isolamento viral Brasil CD4+ linfócitos T HIV Viral isolation Brazil CD4+ Tlymphocytes Arctic medicine. Tropical medicine RC955-962 Carlos Brites Célia Pedroso Nanci Silva Warren D Johnson Jr Roberto Badaró The influence of CD 4+t cells, hiv disease stage and zidovudine on hiv isolation in Bahia, Brazil |
topic_facet |
VIH p24 Isolamento viral Brasil CD4+ linfócitos T HIV Viral isolation Brazil CD4+ Tlymphocytes Arctic medicine. Tropical medicine RC955-962 |
description |
HIV-l isolation was attempted on 72 individuais, including persons with knoum HIV infection and five without proven HIV infection but with indeterminate Western blot patterns, as well as on low-risk HIV seronegative persons. The ahility to detect HIV- 1 frorn culture supernatant by p24 antigen capture assay was evaluated by segregating patients by absolute CD4+ cell counts, clinicai stage of disease, p24 antigenemia and zidovudine use. The likelihood of a p24 positive HIV culture was highest among patients with CD4+ T-cell counts below 200/ul and patients with advanced clinical disease. Use of zidovudine did not affect the rate ofHIV positwity in cultures. Tentativa de isolamento do vírus tipo 1 da imunodeficiência adquirida (VIH-1) foi realizada em 72 indivíduos sendo 51 pacientes com sorologia positiva para o VIH-1, confirmada por Western blot; 5 doadores de sangue com padrão indeterminado ao Western blot; 3 indivíduos com diagnóstico clínico de AIDS, porém com sorologia negativa, e 13 profissionais de saúde soronegativos. Os pacientes foram estratificados de acordo com a contagem de células CD4+, estágio clínico , antigenemia (p24) e uso de zidovudine. As culturas para o VIH-1 foram positivas em 45/50 (90%) tentativas. Houve uma correlação inversa entre o número de células CD4+ e a freqüência de isolamento do VIH-1. As culturas foram positivas em 84% dos indivíduos com CD4+ <200, contra 48% d positividade naqueles com contagem de célula CD4+ acima deste valor. O uso de zidovudine não interferiu na positividade das culturas. Concluímo. que a sensibilidade dos métodos de culture qualitativo e quantitativo é similar para a detecção do VIH-1. A taxa de positividade das culturas não foi afetada pelo uso prévio de zidovudine, mas foi diretamente proporcional ao grau de imunodeficiência dos pacientes. |
format |
Article in Journal/Newspaper |
author |
Carlos Brites Célia Pedroso Nanci Silva Warren D Johnson Jr Roberto Badaró |
author_facet |
Carlos Brites Célia Pedroso Nanci Silva Warren D Johnson Jr Roberto Badaró |
author_sort |
Carlos Brites |
title |
The influence of CD 4+t cells, hiv disease stage and zidovudine on hiv isolation in Bahia, Brazil |
title_short |
The influence of CD 4+t cells, hiv disease stage and zidovudine on hiv isolation in Bahia, Brazil |
title_full |
The influence of CD 4+t cells, hiv disease stage and zidovudine on hiv isolation in Bahia, Brazil |
title_fullStr |
The influence of CD 4+t cells, hiv disease stage and zidovudine on hiv isolation in Bahia, Brazil |
title_full_unstemmed |
The influence of CD 4+t cells, hiv disease stage and zidovudine on hiv isolation in Bahia, Brazil |
title_sort |
influence of cd 4+t cells, hiv disease stage and zidovudine on hiv isolation in bahia, brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
1996 |
url |
https://doaj.org/article/0af90bfaae8144de8157899b6772b0aa |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 29, Iss 1, Pp 5-9 (1996) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821996000100002 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/0af90bfaae8144de8157899b6772b0aa |
_version_ |
1766346550291726336 |