Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama
ABSTRACT Cutaneous leishmaniasis (CL) is an endemic disease in the Republic of Panama, caused by Leishmania (Viannia) parasites, whose most common clinical manifestation is the presence of ulcerated lesions on the skin. These lesions usually present a chronic inflammatory reaction, sometimes granulo...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:0ae8312ee5454b65b595dffa0b1ffae5 2024-09-09T19:26:36+00:00 Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama Kadir González Rosendo Diaz Aurea F. Ferreira Víctor García Héctor Paz José E. Calzada Michelle Ruíz Márcia Laurenti Azael Saldaña 2018-02-01T00:00:00Z https://doi.org/10.1590/s1678-9946201860008 https://doaj.org/article/0ae8312ee5454b65b595dffa0b1ffae5 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652018005000202&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201860008 https://doaj.org/article/0ae8312ee5454b65b595dffa0b1ffae5 Revista do Instituto de Medicina Tropical de São Paulo, Vol 60, Iss 0 (2018) Cutaneous leishmaniasis Leishmania Viannia panamensis Histopathology Panama Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1590/s1678-9946201860008 2024-08-05T17:49:31Z ABSTRACT Cutaneous leishmaniasis (CL) is an endemic disease in the Republic of Panama, caused by Leishmania (Viannia) parasites, whose most common clinical manifestation is the presence of ulcerated lesions on the skin. These lesions usually present a chronic inflammatory reaction, sometimes granulomatous, with the presence of lymphocytes, plasma cells and macrophages. This study describes the histopathological characteristics found in the skin lesions of patients with CL caused by Leishmania (V.) panamensis in Panama. We analyzed 49 skin biopsy samples from patients with clinical suspicion of CL, by molecular tests (PCR for subgenus Viannia and HSP-70) and by Hematoxylin-Eosin staining. Samples were characterized at the species level by PCR-HSP-70/RFLP. From the 49 samples studied, 46 (94%) were positive by PCR and were characterized as Leishmania (V.) panamensis. Of these, 48% were positive by Hematoxylin-Eosin staining with alterations being observed both, in the epidermis (85%) and in the dermis (100%) of skin biopsies. The inflammatory infiltrate was characterized according to histopathological patterns: lymphohistiocytic (50%), lymphoplasmacytic (61%) and granulomatous (46%) infiltration, being the combination of these patterns frequently found. The predominant histopathological characteristics observed in CL lesions caused by L. (V.) panamensis in Panama were: an intense inflammatory reaction in the dermis with a combination of lymphohistiocytic, lymphoplasmacytic and granulomatous presentation patterns and the presence of ulcers, acanthosis, exocytosis and spongiosis in the epidermis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 60 0 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Cutaneous leishmaniasis Leishmania Viannia panamensis Histopathology Panama Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Cutaneous leishmaniasis Leishmania Viannia panamensis Histopathology Panama Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Kadir González Rosendo Diaz Aurea F. Ferreira Víctor García Héctor Paz José E. Calzada Michelle Ruíz Márcia Laurenti Azael Saldaña Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama |
topic_facet |
Cutaneous leishmaniasis Leishmania Viannia panamensis Histopathology Panama Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Cutaneous leishmaniasis (CL) is an endemic disease in the Republic of Panama, caused by Leishmania (Viannia) parasites, whose most common clinical manifestation is the presence of ulcerated lesions on the skin. These lesions usually present a chronic inflammatory reaction, sometimes granulomatous, with the presence of lymphocytes, plasma cells and macrophages. This study describes the histopathological characteristics found in the skin lesions of patients with CL caused by Leishmania (V.) panamensis in Panama. We analyzed 49 skin biopsy samples from patients with clinical suspicion of CL, by molecular tests (PCR for subgenus Viannia and HSP-70) and by Hematoxylin-Eosin staining. Samples were characterized at the species level by PCR-HSP-70/RFLP. From the 49 samples studied, 46 (94%) were positive by PCR and were characterized as Leishmania (V.) panamensis. Of these, 48% were positive by Hematoxylin-Eosin staining with alterations being observed both, in the epidermis (85%) and in the dermis (100%) of skin biopsies. The inflammatory infiltrate was characterized according to histopathological patterns: lymphohistiocytic (50%), lymphoplasmacytic (61%) and granulomatous (46%) infiltration, being the combination of these patterns frequently found. The predominant histopathological characteristics observed in CL lesions caused by L. (V.) panamensis in Panama were: an intense inflammatory reaction in the dermis with a combination of lymphohistiocytic, lymphoplasmacytic and granulomatous presentation patterns and the presence of ulcers, acanthosis, exocytosis and spongiosis in the epidermis. |
format |
Article in Journal/Newspaper |
author |
Kadir González Rosendo Diaz Aurea F. Ferreira Víctor García Héctor Paz José E. Calzada Michelle Ruíz Márcia Laurenti Azael Saldaña |
author_facet |
Kadir González Rosendo Diaz Aurea F. Ferreira Víctor García Héctor Paz José E. Calzada Michelle Ruíz Márcia Laurenti Azael Saldaña |
author_sort |
Kadir González |
title |
Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama |
title_short |
Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama |
title_full |
Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama |
title_fullStr |
Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama |
title_full_unstemmed |
Histopathological characteristics of cutaneous lesions caused by Leishmania Viannia panamensis in Panama |
title_sort |
histopathological characteristics of cutaneous lesions caused by leishmania viannia panamensis in panama |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2018 |
url |
https://doi.org/10.1590/s1678-9946201860008 https://doaj.org/article/0ae8312ee5454b65b595dffa0b1ffae5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 60, Iss 0 (2018) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652018005000202&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201860008 https://doaj.org/article/0ae8312ee5454b65b595dffa0b1ffae5 |
op_doi |
https://doi.org/10.1590/s1678-9946201860008 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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60 |
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