Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain.
Background Although Southeast Asia is one of the most leptospirosis afflicted regions, little is known about the diversity and molecular epidemiology of the causative agents of this widespread and emerging zoonotic disease. Methodology/principal findings We used whole genome sequencing to examine ge...
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ftdoajarticles:oai:doaj.org/article:0ad162bfa4744d08932402642dd3d87e 2023-05-15T15:10:52+02:00 Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. Linda Grillová Matthew T Robinson Anisone Chanthongthip Antony T Vincent Cecilia Nieves Jan Oppelt Jean-François Mariet Céline Lorioux Manivanh Vongsouvath Mayfong Mayxay Ooyanong Phonemeexay Sayaphet Rattanavong Koukeo Phommasone Anousone Douangnouvong David Šmajs Frédéric J Veyrier Paul N Newton Mathieu Picardeau 2021-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010076 https://doaj.org/article/0ad162bfa4744d08932402642dd3d87e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010076 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010076 https://doaj.org/article/0ad162bfa4744d08932402642dd3d87e PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0010076 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010076 2022-12-31T11:06:36Z Background Although Southeast Asia is one of the most leptospirosis afflicted regions, little is known about the diversity and molecular epidemiology of the causative agents of this widespread and emerging zoonotic disease. Methodology/principal findings We used whole genome sequencing to examine genetic variation in 75 Leptospira strains isolated from patients in the Lao PDR (Laos) between 2006 and 2017. Eleven serogroups from 4 Leptospira species and 43 cgMLST-defined clonal groups (CGs) were identified. The most prevalent CG was CG272 (n = 18, 26.8%), composed of L. interrogans serogroup Autumnalis isolates. This genotype was recovered throughout the 12-year period and was associated with deaths, and with a large outbreak in neighbouring Thailand. Genome analysis reveals that the CG272 strains form a highly clonal group of strains that have, for yet unknown reasons, recently spread in Laos and Thailand. Additionally, accessory genes clearly discriminate CG272 strains from the other Leptospira strains. Conclusions/significance The present study reveals a high diversity of Leptospira genotypes in Laos, thus extending our current knowledge of the pan- and core-genomes of these life-threatening pathogens. Our results demonstrate that the CG272 strains belong to a unique clonal group, which probably evolved through clonal expansion following niche adaptation. Additional epidemiological studies are required to better evaluate the spread of this genotype in Southeast Asia. To further investigate the key factors driving the virulence and spread of these pathogens, more intense genomic surveillance is needed, combining detailed clinical and epidemiological data. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 12 e0010076 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Linda Grillová Matthew T Robinson Anisone Chanthongthip Antony T Vincent Cecilia Nieves Jan Oppelt Jean-François Mariet Céline Lorioux Manivanh Vongsouvath Mayfong Mayxay Ooyanong Phonemeexay Sayaphet Rattanavong Koukeo Phommasone Anousone Douangnouvong David Šmajs Frédéric J Veyrier Paul N Newton Mathieu Picardeau Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Although Southeast Asia is one of the most leptospirosis afflicted regions, little is known about the diversity and molecular epidemiology of the causative agents of this widespread and emerging zoonotic disease. Methodology/principal findings We used whole genome sequencing to examine genetic variation in 75 Leptospira strains isolated from patients in the Lao PDR (Laos) between 2006 and 2017. Eleven serogroups from 4 Leptospira species and 43 cgMLST-defined clonal groups (CGs) were identified. The most prevalent CG was CG272 (n = 18, 26.8%), composed of L. interrogans serogroup Autumnalis isolates. This genotype was recovered throughout the 12-year period and was associated with deaths, and with a large outbreak in neighbouring Thailand. Genome analysis reveals that the CG272 strains form a highly clonal group of strains that have, for yet unknown reasons, recently spread in Laos and Thailand. Additionally, accessory genes clearly discriminate CG272 strains from the other Leptospira strains. Conclusions/significance The present study reveals a high diversity of Leptospira genotypes in Laos, thus extending our current knowledge of the pan- and core-genomes of these life-threatening pathogens. Our results demonstrate that the CG272 strains belong to a unique clonal group, which probably evolved through clonal expansion following niche adaptation. Additional epidemiological studies are required to better evaluate the spread of this genotype in Southeast Asia. To further investigate the key factors driving the virulence and spread of these pathogens, more intense genomic surveillance is needed, combining detailed clinical and epidemiological data. |
format |
Article in Journal/Newspaper |
author |
Linda Grillová Matthew T Robinson Anisone Chanthongthip Antony T Vincent Cecilia Nieves Jan Oppelt Jean-François Mariet Céline Lorioux Manivanh Vongsouvath Mayfong Mayxay Ooyanong Phonemeexay Sayaphet Rattanavong Koukeo Phommasone Anousone Douangnouvong David Šmajs Frédéric J Veyrier Paul N Newton Mathieu Picardeau |
author_facet |
Linda Grillová Matthew T Robinson Anisone Chanthongthip Antony T Vincent Cecilia Nieves Jan Oppelt Jean-François Mariet Céline Lorioux Manivanh Vongsouvath Mayfong Mayxay Ooyanong Phonemeexay Sayaphet Rattanavong Koukeo Phommasone Anousone Douangnouvong David Šmajs Frédéric J Veyrier Paul N Newton Mathieu Picardeau |
author_sort |
Linda Grillová |
title |
Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. |
title_short |
Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. |
title_full |
Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. |
title_fullStr |
Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. |
title_full_unstemmed |
Genetic diversity of Leptospira isolates in Lao PDR and genome analysis of an outbreak strain. |
title_sort |
genetic diversity of leptospira isolates in lao pdr and genome analysis of an outbreak strain. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0010076 https://doaj.org/article/0ad162bfa4744d08932402642dd3d87e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0010076 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010076 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010076 https://doaj.org/article/0ad162bfa4744d08932402642dd3d87e |
op_doi |
https://doi.org/10.1371/journal.pntd.0010076 |
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PLOS Neglected Tropical Diseases |
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15 |
container_issue |
12 |
container_start_page |
e0010076 |
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