Tidal effect on environmental DNA communities in Arctic estuarine and marine ecosystems

• Published in: Frontiers in Marine Science
• Main Authors: Maelle Sevellec, Anaïs Lacoursière-Roussel, Eric Normandeau, Louis Bernatchez, Kimberly Howland
• Format: Article in Journal/Newspaper
• Language: English
• Published: Frontiers Media S.A. 2024
• Subjects: Arctic; estuarine and marine ecosystems; eDNA dynamic; metabarcoding; tidal effect; coastal communities; Science; Q; General. Including nature conservation; geographical distribution; QH1-199.5; Churchill; Climate change; Nunavut
• Online Access: https://doi.org/10.3389/fmars.2024.1390489; https://doaj.org/article/0ac214912bef4dd3becea0d8b7cc20a8

IntroductionArctic marine ecosystems are changing rapidly, largely due to the observed accelerated warming that is associated with ongoing climate change. Environmental DNA (eDNA) combined with metabarcoding has great potential for large-scale biomonitoring of Arctic marine communities. However, important limitations remain, such as understanding the complexity and drivers of spatio-temporal variation in eDNA distribution.MethodsIn this study, we investigated the effect of tidal dynamics on aquatic metazoan (vertebrates and invertebrates) on eDNA metabarcoding results from nearshore estuarine and marine Arctic ports of Churchill (Manitoba) and Milne Inlet (Nunavut), respectively. We collected and sequenced 54 water samples per port at low, middle and high tide across three days, as well as two depths (surface, bottom), using four universal primer pairs (two primers in the COI gene and two in the 18S rRNA gene).ResultsWe observed a significant transition in the estuarine community structure from low to high tide, whereas the marine community structure was more stable across tides. The eDNA community structure differed between the surface and bottom waters in both the estuarine and marine ecosystems. However, the biodiversity pattern within the water column was significantly different between estuarine and marine ecosystems. Finally, we observed short-term temporal variation of the communities in both systems.DiscussionAltogether, our results highlight the short-term temporal dynamic nature of eDNA derived from coastal communities. This variability should be accounted for in eDNA sampling design to ensure robust characterization of coastal communities and long-term time series, particularly for estuarine environments where the effects of tide and depth are more important.