Insights into Early Ontogenesis of Salmo salar : RNA Extraction, Housekeeping Gene Validation and Transcriptional Expression of Important Primordial Germ Cell and Sex-Determination Genes

• Published in: Animals
• Main Authors: Irfan Ahmad Bhat, Milena Malgorzata Dubiel, Eduardo Rodriguez, Zophonías Oddur Jónsson
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2023
• Subjects: PGCs; gonads; sex determination; housekeeping genes; S. salar; sterility; Veterinary medicine; SF600-1100; Zoology; QL1-991; Vasa; Salmo salar
• Online Access: https://doi.org/10.3390/ani13061094; https://doaj.org/article/0ab6ce6f6e5e400fa1db309f4ffd7737

The challenge in extracting high-quality RNA impedes the investigation of the transcriptome of developing salmonid embryos. Furthermore, the mRNA expression pattern of important PGC and SD genes during the initial embryonic development of Salmo salar is yet to be studied. So, in the present study, w...