MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition
Abstract Background Lymph node and distant metastasis contribute to poor outcomes in patients with oral squamous cell carcinoma (OSCC). The mechanisms regulating cancer migration and invasion play a key role in OSCC. Methods We determined migration and invasion ability of OSCC by wound-healing assay...
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ftdoajarticles:oai:doaj.org/article:0aac509a7647492fab204db355a88715 2023-05-15T17:54:02+02:00 MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition Sheng-Yen Hsiao Shang-Mei Weng Jenn-Ren Hsiao Yi-Ying Wu Jia-En Wu Chia-Hao Tung Wan-Lin Shen Shu-Fang Sun Wen-Tsung Huang Cheng-Yao Lin Shang-Hung Chen Tse-Ming Hong Yuh-Ling Chen Jang-Yang Chang 2023-02-01T00:00:00Z https://doi.org/10.1186/s13046-023-02597-1 https://doaj.org/article/0aac509a7647492fab204db355a88715 EN eng BMC https://doi.org/10.1186/s13046-023-02597-1 https://doaj.org/toc/1756-9966 doi:10.1186/s13046-023-02597-1 1756-9966 https://doaj.org/article/0aac509a7647492fab204db355a88715 Journal of Experimental & Clinical Cancer Research, Vol 42, Iss 1, Pp 1-19 (2023) miR-455-5p PDZK1IP1 Partial epithelial-to-mesenchymal transition Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 article 2023 ftdoajarticles https://doi.org/10.1186/s13046-023-02597-1 2023-02-12T01:32:23Z Abstract Background Lymph node and distant metastasis contribute to poor outcomes in patients with oral squamous cell carcinoma (OSCC). The mechanisms regulating cancer migration and invasion play a key role in OSCC. Methods We determined migration and invasion ability of OSCC by wound-healing assay, two-chamber transwell invasion assay and cell mobility tracking and evaluated tumor metastasis in vivo. Western blot (WB), qRT-PCR, RNA-seq, dual-luciferase reporter assays and nuclear/cytoplasmic fractionation were performed to investigate the potential mechanism. Immunohistochimical (IHC) staining determined vimentin and PDZK1IP1 expression in OSCC tissues. Results and conclusion In this study, we determined that miR-455-5p was associated with lymph node metastasis and clinical invasion, leading to poor outcomes in patients with OSCC. MiR-455-5p promoted oral cancer cell migration and invasion and induced epithelial-to-mesenchymal transition (EMT). We also identified a new biomarker, PDZK1IP1 (MAP17), that was targeted by miR-455-5p. PDZK1IP1 knockdown led to migration, metastasis, EMT, and increased transforming growth factor-β signaling in OSCC. In addition, miR-455-5p overexpression and PDZK1IP1 inhibition promoted collective OSCC cell migration. According to data from the Cancer Genome Atlas database and the NCKU-OrCA-40TN data set, miR-455-5p and PDZK1IP1 are positively and negatively correlated, respectively, with partial EMT score. High miR-455-5p expression was associated with high vimentin levels and low MAP17 H-scores. The patients with low MAP17 expression had higher rates of disease recurrence than did patients with high MAP17 expression, especially for patients with clinical invasion risk factors and low MAP17 expression. These results suggest that miR-455-5p suppresses PDZK1IP1 expression and mediates OSCC progression. MiR-455-5p and PDZK1IP1 may therefore serve as key biomarkers and be involved in regulating partial EMT in OSCC cells. PDZK1IP1 expression may also serve as an independent factor that ... Article in Journal/Newspaper Orca Directory of Open Access Journals: DOAJ Articles Journal of Experimental & Clinical Cancer Research 42 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
miR-455-5p PDZK1IP1 Partial epithelial-to-mesenchymal transition Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 |
spellingShingle |
miR-455-5p PDZK1IP1 Partial epithelial-to-mesenchymal transition Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Sheng-Yen Hsiao Shang-Mei Weng Jenn-Ren Hsiao Yi-Ying Wu Jia-En Wu Chia-Hao Tung Wan-Lin Shen Shu-Fang Sun Wen-Tsung Huang Cheng-Yao Lin Shang-Hung Chen Tse-Ming Hong Yuh-Ling Chen Jang-Yang Chang MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition |
topic_facet |
miR-455-5p PDZK1IP1 Partial epithelial-to-mesenchymal transition Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 |
description |
Abstract Background Lymph node and distant metastasis contribute to poor outcomes in patients with oral squamous cell carcinoma (OSCC). The mechanisms regulating cancer migration and invasion play a key role in OSCC. Methods We determined migration and invasion ability of OSCC by wound-healing assay, two-chamber transwell invasion assay and cell mobility tracking and evaluated tumor metastasis in vivo. Western blot (WB), qRT-PCR, RNA-seq, dual-luciferase reporter assays and nuclear/cytoplasmic fractionation were performed to investigate the potential mechanism. Immunohistochimical (IHC) staining determined vimentin and PDZK1IP1 expression in OSCC tissues. Results and conclusion In this study, we determined that miR-455-5p was associated with lymph node metastasis and clinical invasion, leading to poor outcomes in patients with OSCC. MiR-455-5p promoted oral cancer cell migration and invasion and induced epithelial-to-mesenchymal transition (EMT). We also identified a new biomarker, PDZK1IP1 (MAP17), that was targeted by miR-455-5p. PDZK1IP1 knockdown led to migration, metastasis, EMT, and increased transforming growth factor-β signaling in OSCC. In addition, miR-455-5p overexpression and PDZK1IP1 inhibition promoted collective OSCC cell migration. According to data from the Cancer Genome Atlas database and the NCKU-OrCA-40TN data set, miR-455-5p and PDZK1IP1 are positively and negatively correlated, respectively, with partial EMT score. High miR-455-5p expression was associated with high vimentin levels and low MAP17 H-scores. The patients with low MAP17 expression had higher rates of disease recurrence than did patients with high MAP17 expression, especially for patients with clinical invasion risk factors and low MAP17 expression. These results suggest that miR-455-5p suppresses PDZK1IP1 expression and mediates OSCC progression. MiR-455-5p and PDZK1IP1 may therefore serve as key biomarkers and be involved in regulating partial EMT in OSCC cells. PDZK1IP1 expression may also serve as an independent factor that ... |
format |
Article in Journal/Newspaper |
author |
Sheng-Yen Hsiao Shang-Mei Weng Jenn-Ren Hsiao Yi-Ying Wu Jia-En Wu Chia-Hao Tung Wan-Lin Shen Shu-Fang Sun Wen-Tsung Huang Cheng-Yao Lin Shang-Hung Chen Tse-Ming Hong Yuh-Ling Chen Jang-Yang Chang |
author_facet |
Sheng-Yen Hsiao Shang-Mei Weng Jenn-Ren Hsiao Yi-Ying Wu Jia-En Wu Chia-Hao Tung Wan-Lin Shen Shu-Fang Sun Wen-Tsung Huang Cheng-Yao Lin Shang-Hung Chen Tse-Ming Hong Yuh-Ling Chen Jang-Yang Chang |
author_sort |
Sheng-Yen Hsiao |
title |
MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition |
title_short |
MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition |
title_full |
MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition |
title_fullStr |
MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition |
title_full_unstemmed |
MiR-455-5p suppresses PDZK1IP1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition |
title_sort |
mir-455-5p suppresses pdzk1ip1 to promote the motility of oral squamous cell carcinoma and accelerate clinical cancer invasion by regulating partial epithelial-to-mesenchymal transition |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s13046-023-02597-1 https://doaj.org/article/0aac509a7647492fab204db355a88715 |
genre |
Orca |
genre_facet |
Orca |
op_source |
Journal of Experimental & Clinical Cancer Research, Vol 42, Iss 1, Pp 1-19 (2023) |
op_relation |
https://doi.org/10.1186/s13046-023-02597-1 https://doaj.org/toc/1756-9966 doi:10.1186/s13046-023-02597-1 1756-9966 https://doaj.org/article/0aac509a7647492fab204db355a88715 |
op_doi |
https://doi.org/10.1186/s13046-023-02597-1 |
container_title |
Journal of Experimental & Clinical Cancer Research |
container_volume |
42 |
container_issue |
1 |
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1766161755171454976 |