Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria
Abstract Background The erythrocytic stage, where malaria parasites proliferate in human blood, is clinically significant as this causes the symptoms and illness of malaria. Experimental rodent models of malaria at the erythrocytic stage are used for the development of anti-malarial drugs and for bi...
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ftdoajarticles:oai:doaj.org/article:0a7ac2c570ff4b708df9c5295101a310 2023-05-15T15:12:37+02:00 Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria Takahiro Tougan Yuhgi Suzuki Munehisa Izuka Kei Aono Tomonori Okazaki Yuji Toya Kinya Uchihashi Toshihiro Horii 2018-04-01T00:00:00Z https://doi.org/10.1186/s12936-018-2313-6 https://doaj.org/article/0a7ac2c570ff4b708df9c5295101a310 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2313-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2313-6 1475-2875 https://doaj.org/article/0a7ac2c570ff4b708df9c5295101a310 Malaria Journal, Vol 17, Iss 1, Pp 1-11 (2018) Rodent malaria parasite Mouse Blood stage Parasitaemia Haematological state Howell–Jolly body Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2313-6 2022-12-30T22:00:38Z Abstract Background The erythrocytic stage, where malaria parasites proliferate in human blood, is clinically significant as this causes the symptoms and illness of malaria. Experimental rodent models of malaria at the erythrocytic stage are used for the development of anti-malarial drugs and for biological analysis. An automated haematology analyzer XN-30 was developed for detection of infected red blood cells (iRBCs) in human blood samples and measurement of their parasitaemia in approximately 1 min through flow cytometry analysis. Additionally, the analyzer simultaneously measured other haematological parameters in these samples. It is inferred that the analyzer would also allow easy and rapid measurement of parasitaemia in mice and provide important clues on the mouse haematological state during infection and treatment. Results The XN-30 analyzer is a simple and rapid tool to detect iRBCs in mouse blood samples infected with rodent malarial parasites, with three-dimensional analysis permitting the precise measurement of parasitaemia (referred herein as the ‘XN-30 system’). The XN-30 analyzer allowed not only the detection of iRBCs but also the monitoring of RBC, white blood cell, and platelet counts, as well as haematocrit, mean corpuscular volume and mean platelet volume values in the mouse blood sample. For anti-malarial drug development, aside from demonstrating possible efficacy in mouse models, XN-30 analyzer could provide a first glimpse of the safety profile of the drug. Conclusions The XN-30 system is a powerful tool that can be utilized for the in vivo screening, development, and evaluation of anti-malarial drugs as well as for pre-clinical pharmacology and/or toxicity tests in rodent models. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Howell ENVELOPE(-99.050,-99.050,-72.233,-72.233) Malaria Journal 17 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Rodent malaria parasite Mouse Blood stage Parasitaemia Haematological state Howell–Jolly body Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Rodent malaria parasite Mouse Blood stage Parasitaemia Haematological state Howell–Jolly body Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Takahiro Tougan Yuhgi Suzuki Munehisa Izuka Kei Aono Tomonori Okazaki Yuji Toya Kinya Uchihashi Toshihiro Horii Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria |
topic_facet |
Rodent malaria parasite Mouse Blood stage Parasitaemia Haematological state Howell–Jolly body Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The erythrocytic stage, where malaria parasites proliferate in human blood, is clinically significant as this causes the symptoms and illness of malaria. Experimental rodent models of malaria at the erythrocytic stage are used for the development of anti-malarial drugs and for biological analysis. An automated haematology analyzer XN-30 was developed for detection of infected red blood cells (iRBCs) in human blood samples and measurement of their parasitaemia in approximately 1 min through flow cytometry analysis. Additionally, the analyzer simultaneously measured other haematological parameters in these samples. It is inferred that the analyzer would also allow easy and rapid measurement of parasitaemia in mice and provide important clues on the mouse haematological state during infection and treatment. Results The XN-30 analyzer is a simple and rapid tool to detect iRBCs in mouse blood samples infected with rodent malarial parasites, with three-dimensional analysis permitting the precise measurement of parasitaemia (referred herein as the ‘XN-30 system’). The XN-30 analyzer allowed not only the detection of iRBCs but also the monitoring of RBC, white blood cell, and platelet counts, as well as haematocrit, mean corpuscular volume and mean platelet volume values in the mouse blood sample. For anti-malarial drug development, aside from demonstrating possible efficacy in mouse models, XN-30 analyzer could provide a first glimpse of the safety profile of the drug. Conclusions The XN-30 system is a powerful tool that can be utilized for the in vivo screening, development, and evaluation of anti-malarial drugs as well as for pre-clinical pharmacology and/or toxicity tests in rodent models. |
format |
Article in Journal/Newspaper |
author |
Takahiro Tougan Yuhgi Suzuki Munehisa Izuka Kei Aono Tomonori Okazaki Yuji Toya Kinya Uchihashi Toshihiro Horii |
author_facet |
Takahiro Tougan Yuhgi Suzuki Munehisa Izuka Kei Aono Tomonori Okazaki Yuji Toya Kinya Uchihashi Toshihiro Horii |
author_sort |
Takahiro Tougan |
title |
Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria |
title_short |
Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria |
title_full |
Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria |
title_fullStr |
Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria |
title_full_unstemmed |
Application of the automated haematology analyzer XN-30 in an experimental rodent model of malaria |
title_sort |
application of the automated haematology analyzer xn-30 in an experimental rodent model of malaria |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2313-6 https://doaj.org/article/0a7ac2c570ff4b708df9c5295101a310 |
long_lat |
ENVELOPE(-99.050,-99.050,-72.233,-72.233) |
geographic |
Arctic Howell |
geographic_facet |
Arctic Howell |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-11 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2313-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2313-6 1475-2875 https://doaj.org/article/0a7ac2c570ff4b708df9c5295101a310 |
op_doi |
https://doi.org/10.1186/s12936-018-2313-6 |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
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1766343277335805952 |