Detection of Coronaviruses in Bats of Various Species in Italy
Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization...
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ftdoajarticles:oai:doaj.org/article:0a7a0a5e967147bc9403c939039ad3ab 2023-05-15T17:48:37+02:00 Detection of Coronaviruses in Bats of Various Species in Italy Maria B. Boniotti Enrica Rosti Ana Moreno Cristiano Sabelli Alice Papetti Davide Lelli 2013-10-01T00:00:00Z https://doi.org/10.3390/v5112679 https://doaj.org/article/0a7a0a5e967147bc9403c939039ad3ab EN eng MDPI AG http://www.mdpi.com/1999-4915/5/11/2679 https://doaj.org/toc/1999-4915 doi:10.3390/v5112679 1999-4915 https://doaj.org/article/0a7a0a5e967147bc9403c939039ad3ab Viruses, Vol 5, Iss 11, Pp 2679-2689 (2013) coronavirus bats Italy molecular characterization phylogenetic analysis Microbiology QR1-502 article 2013 ftdoajarticles https://doi.org/10.3390/v5112679 2022-12-31T10:54:50Z Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization of alphacoronaviruses and betacoronaviruses in bats in Italy, from 2010 to 2012. Sixty-nine faecal samples and 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs were detected in seven faecal samples and nine carcasses. A phylogenetic analysis of RNA-dependent RNA polymerase sequence fragments aided in identifying two alphacoronaviruses from Kuhl’s pipistrelle (Pipistrellus kuhlii), three clade 2b betacoronaviruses from lesser horseshoe bats (Rhinolophus hipposideros), and 10 clade 2c betacoronaviruses from Kuhl’s pipistrelle, common noctule (Nyctalus noctula), and Savi’s pipistrelle (Hypsugo savii). This study fills a substantive gap in the knowledge on bat-CoV ecology in Italy, and extends the current knowledge on clade 2c betacoronaviruses with new sequences obtained from bats that have not been previously described as hosts of these viruses. Article in Journal/Newspaper Nyctalus noctula Directory of Open Access Journals: DOAJ Articles Viruses 5 11 2679 2689 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
coronavirus bats Italy molecular characterization phylogenetic analysis Microbiology QR1-502 |
spellingShingle |
coronavirus bats Italy molecular characterization phylogenetic analysis Microbiology QR1-502 Maria B. Boniotti Enrica Rosti Ana Moreno Cristiano Sabelli Alice Papetti Davide Lelli Detection of Coronaviruses in Bats of Various Species in Italy |
topic_facet |
coronavirus bats Italy molecular characterization phylogenetic analysis Microbiology QR1-502 |
description |
Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization of alphacoronaviruses and betacoronaviruses in bats in Italy, from 2010 to 2012. Sixty-nine faecal samples and 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs were detected in seven faecal samples and nine carcasses. A phylogenetic analysis of RNA-dependent RNA polymerase sequence fragments aided in identifying two alphacoronaviruses from Kuhl’s pipistrelle (Pipistrellus kuhlii), three clade 2b betacoronaviruses from lesser horseshoe bats (Rhinolophus hipposideros), and 10 clade 2c betacoronaviruses from Kuhl’s pipistrelle, common noctule (Nyctalus noctula), and Savi’s pipistrelle (Hypsugo savii). This study fills a substantive gap in the knowledge on bat-CoV ecology in Italy, and extends the current knowledge on clade 2c betacoronaviruses with new sequences obtained from bats that have not been previously described as hosts of these viruses. |
format |
Article in Journal/Newspaper |
author |
Maria B. Boniotti Enrica Rosti Ana Moreno Cristiano Sabelli Alice Papetti Davide Lelli |
author_facet |
Maria B. Boniotti Enrica Rosti Ana Moreno Cristiano Sabelli Alice Papetti Davide Lelli |
author_sort |
Maria B. Boniotti |
title |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_short |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_full |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_fullStr |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_full_unstemmed |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_sort |
detection of coronaviruses in bats of various species in italy |
publisher |
MDPI AG |
publishDate |
2013 |
url |
https://doi.org/10.3390/v5112679 https://doaj.org/article/0a7a0a5e967147bc9403c939039ad3ab |
genre |
Nyctalus noctula |
genre_facet |
Nyctalus noctula |
op_source |
Viruses, Vol 5, Iss 11, Pp 2679-2689 (2013) |
op_relation |
http://www.mdpi.com/1999-4915/5/11/2679 https://doaj.org/toc/1999-4915 doi:10.3390/v5112679 1999-4915 https://doaj.org/article/0a7a0a5e967147bc9403c939039ad3ab |
op_doi |
https://doi.org/10.3390/v5112679 |
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Viruses |
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5 |
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11 |
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2679 |
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2689 |
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1766154749596401664 |