Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response
Abstract Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods Africanized hon...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:0a794f88c7f945dfaebc3e6731ddc632 2023-05-15T15:07:37+02:00 Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response Andreia Vieira Pereira Gustavo de Barros Erika Gracielle Pinto Andre Gustavo Tempone Ricardo de Oliveira Orsi Lucilene Delazari dos Santos Sueli Calvi Rui Seabra Ferreira Jr Daniel Carvalho Pimenta Benedito Barraviera 2016-02-01T00:00:00Z https://doi.org/10.1186/s40409-016-0055-x https://doaj.org/article/0a794f88c7f945dfaebc3e6731ddc632 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-016-0055-x https://doaj.org/article/0a794f88c7f945dfaebc3e6731ddc632 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 22, Iss 0 (2016) Melittin Apis mellifera Leishmania Leishmaniasis Peptides Toxins Antiparasitic Cytokines Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2016 ftdoajarticles https://doi.org/10.1186/s40409-016-0055-x 2022-12-31T04:43:12Z Abstract Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results Promastigotes and intracellular amastigotes were susceptible to melittin (IC50 28.3 μg.mL−1 and 1.4 μg.mL−1, respectively), but also showed mammalian cell cytotoxicity with an IC50 value of 5.7 μg.mL−1. Uninfected macrophages treated with melittin increased the production of IL-10, TNF-α, NO and H2O2. Infected melittin-treated macrophages increased IL-12 production, but decreased the levels of IL-10, TNF-α, NO and H2O2. Conclusions The results showed that melittin acts in vitro against promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Furthermore, they can act indirectly on intracellular amastigotes through a macrophage immunomodulatory effect. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 22 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Melittin Apis mellifera Leishmania Leishmaniasis Peptides Toxins Antiparasitic Cytokines Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Melittin Apis mellifera Leishmania Leishmaniasis Peptides Toxins Antiparasitic Cytokines Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Andreia Vieira Pereira Gustavo de Barros Erika Gracielle Pinto Andre Gustavo Tempone Ricardo de Oliveira Orsi Lucilene Delazari dos Santos Sueli Calvi Rui Seabra Ferreira Jr Daniel Carvalho Pimenta Benedito Barraviera Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response |
topic_facet |
Melittin Apis mellifera Leishmania Leishmaniasis Peptides Toxins Antiparasitic Cytokines Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results Promastigotes and intracellular amastigotes were susceptible to melittin (IC50 28.3 μg.mL−1 and 1.4 μg.mL−1, respectively), but also showed mammalian cell cytotoxicity with an IC50 value of 5.7 μg.mL−1. Uninfected macrophages treated with melittin increased the production of IL-10, TNF-α, NO and H2O2. Infected melittin-treated macrophages increased IL-12 production, but decreased the levels of IL-10, TNF-α, NO and H2O2. Conclusions The results showed that melittin acts in vitro against promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Furthermore, they can act indirectly on intracellular amastigotes through a macrophage immunomodulatory effect. |
format |
Article in Journal/Newspaper |
author |
Andreia Vieira Pereira Gustavo de Barros Erika Gracielle Pinto Andre Gustavo Tempone Ricardo de Oliveira Orsi Lucilene Delazari dos Santos Sueli Calvi Rui Seabra Ferreira Jr Daniel Carvalho Pimenta Benedito Barraviera |
author_facet |
Andreia Vieira Pereira Gustavo de Barros Erika Gracielle Pinto Andre Gustavo Tempone Ricardo de Oliveira Orsi Lucilene Delazari dos Santos Sueli Calvi Rui Seabra Ferreira Jr Daniel Carvalho Pimenta Benedito Barraviera |
author_sort |
Andreia Vieira Pereira |
title |
Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response |
title_short |
Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response |
title_full |
Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response |
title_fullStr |
Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response |
title_full_unstemmed |
Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response |
title_sort |
melittin induces in vitro death of leishmania (leishmania) infantum by triggering the cellular innate immune response |
publisher |
SciELO |
publishDate |
2016 |
url |
https://doi.org/10.1186/s40409-016-0055-x https://doaj.org/article/0a794f88c7f945dfaebc3e6731ddc632 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 22, Iss 0 (2016) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-016-0055-x https://doaj.org/article/0a794f88c7f945dfaebc3e6731ddc632 |
op_doi |
https://doi.org/10.1186/s40409-016-0055-x |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
22 |
container_issue |
1 |
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1766339088890200064 |