Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels.
Seminal plasma (SP) plays an important role in the motility, viability and maintenance of the fertilizing capacity of mammalian spermatozoa. This study is the first on brown bear (Ursus arctos) SP components, and has two main objectives: 1) to define the SP composition in bear ejaculate and 2) to id...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0181776 https://doaj.org/article/0a692f702f3e46759dc64c1ce1f7524d |
id |
ftdoajarticles:oai:doaj.org/article:0a692f702f3e46759dc64c1ce1f7524d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0a692f702f3e46759dc64c1ce1f7524d 2023-05-15T18:41:51+02:00 Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels. L Anel-López C Ortega-Ferrusola C Martínez-Rodríguez M Álvarez S Borragán C Chamorro F J Peña L Anel P de Paz 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0181776 https://doaj.org/article/0a692f702f3e46759dc64c1ce1f7524d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5542667?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0181776 https://doaj.org/article/0a692f702f3e46759dc64c1ce1f7524d PLoS ONE, Vol 12, Iss 8, p e0181776 (2017) Medicine R Science Q article 2017 ftdoajarticles https://doi.org/10.1371/journal.pone.0181776 2022-12-31T13:33:02Z Seminal plasma (SP) plays an important role in the motility, viability and maintenance of the fertilizing capacity of mammalian spermatozoa. This study is the first on brown bear (Ursus arctos) SP components, and has two main objectives: 1) to define the SP composition in bear ejaculate and 2) to identify variations in SP composition in relation to high and low levels of testosterone in serum during the breeding season. Forty-eight sperm samples from 30 sexually mature male brown bears (Ursus arctos) were obtained by electroejaculation, and their serum testosterone levels were assessed to sort the animals into 2 groups (high and low testosterone levels, threshold 5 ng/dl). The biochemical and protein compositions of the SP samples were assessed, and sperm motility was analyzed. We found that lactate dehydrogenase was significantly higher in the low-serum-testosterone samples, while concentrations of lipase and Mg+ values were significantly higher in the high-serum-testosterone samples. In contrast, sperm motility did not significantly differ (P>0.05) between the testosterone level groups (total motility: 74.42.8% in the high-level group vs. 77.1±4.7% in the low-level group). A reference digital model was constructed since there is no information for this wild species. To do this, all gel images were added in a binary multidimensional image and thirty-three spots were identified as the most-repeated spots. An analysis of these proteins was done by qualitative equivalency (isoelectric point and molecular weight) with published data for a bull. SP protein composition was compared between bears with high and low serum testosterone, and three proteins (binder of sperm and two enzymes not identified in the reference bull) showed significant (P<0.05) quantitative differences. We conclude that male bears with high or low serum testosterone levels differs only in some properties of their SP, differences in enzyme LDIP2, energy source LACT2, one protein (similar to BSP1) and Mg ion were identified between these two ... Article in Journal/Newspaper Ursus arctos Directory of Open Access Journals: DOAJ Articles PLOS ONE 12 8 e0181776 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q L Anel-López C Ortega-Ferrusola C Martínez-Rodríguez M Álvarez S Borragán C Chamorro F J Peña L Anel P de Paz Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels. |
topic_facet |
Medicine R Science Q |
description |
Seminal plasma (SP) plays an important role in the motility, viability and maintenance of the fertilizing capacity of mammalian spermatozoa. This study is the first on brown bear (Ursus arctos) SP components, and has two main objectives: 1) to define the SP composition in bear ejaculate and 2) to identify variations in SP composition in relation to high and low levels of testosterone in serum during the breeding season. Forty-eight sperm samples from 30 sexually mature male brown bears (Ursus arctos) were obtained by electroejaculation, and their serum testosterone levels were assessed to sort the animals into 2 groups (high and low testosterone levels, threshold 5 ng/dl). The biochemical and protein compositions of the SP samples were assessed, and sperm motility was analyzed. We found that lactate dehydrogenase was significantly higher in the low-serum-testosterone samples, while concentrations of lipase and Mg+ values were significantly higher in the high-serum-testosterone samples. In contrast, sperm motility did not significantly differ (P>0.05) between the testosterone level groups (total motility: 74.42.8% in the high-level group vs. 77.1±4.7% in the low-level group). A reference digital model was constructed since there is no information for this wild species. To do this, all gel images were added in a binary multidimensional image and thirty-three spots were identified as the most-repeated spots. An analysis of these proteins was done by qualitative equivalency (isoelectric point and molecular weight) with published data for a bull. SP protein composition was compared between bears with high and low serum testosterone, and three proteins (binder of sperm and two enzymes not identified in the reference bull) showed significant (P<0.05) quantitative differences. We conclude that male bears with high or low serum testosterone levels differs only in some properties of their SP, differences in enzyme LDIP2, energy source LACT2, one protein (similar to BSP1) and Mg ion were identified between these two ... |
format |
Article in Journal/Newspaper |
author |
L Anel-López C Ortega-Ferrusola C Martínez-Rodríguez M Álvarez S Borragán C Chamorro F J Peña L Anel P de Paz |
author_facet |
L Anel-López C Ortega-Ferrusola C Martínez-Rodríguez M Álvarez S Borragán C Chamorro F J Peña L Anel P de Paz |
author_sort |
L Anel-López |
title |
Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels. |
title_short |
Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels. |
title_full |
Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels. |
title_fullStr |
Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels. |
title_full_unstemmed |
Analysis of seminal plasma from brown bear (Ursus arctos) during the breeding season: Its relationship with testosterone levels. |
title_sort |
analysis of seminal plasma from brown bear (ursus arctos) during the breeding season: its relationship with testosterone levels. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pone.0181776 https://doaj.org/article/0a692f702f3e46759dc64c1ce1f7524d |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
PLoS ONE, Vol 12, Iss 8, p e0181776 (2017) |
op_relation |
http://europepmc.org/articles/PMC5542667?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0181776 https://doaj.org/article/0a692f702f3e46759dc64c1ce1f7524d |
op_doi |
https://doi.org/10.1371/journal.pone.0181776 |
container_title |
PLOS ONE |
container_volume |
12 |
container_issue |
8 |
container_start_page |
e0181776 |
_version_ |
1766231417342132224 |