Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin
Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi...
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ftdoajarticles:oai:doaj.org/article:0a62c14439c8436daedf2ed62e0bf37c 2023-05-15T13:33:54+02:00 Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin Anaí Díaz Pablo Villanueva Vicente Oliva Carlos Gil-Durán Francisco Fierro Renato Chávez Inmaculada Vaca 2019-11-01T00:00:00Z https://doi.org/10.3389/fmicb.2019.02675 https://doaj.org/article/0a62c14439c8436daedf2ed62e0bf37c EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2019.02675/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.02675 https://doaj.org/article/0a62c14439c8436daedf2ed62e0bf37c Frontiers in Microbiology, Vol 10 (2019) transformation Pseudogymnoascus protoplasts electroporation Antarctica Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.3389/fmicb.2019.02675 2022-12-31T05:36:56Z Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of Pseudogymnoascus strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a Pseudogymnoascus verrucosus strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of Pseudogymnoascus verrucosus described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Frontiers in Microbiology 10 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
transformation Pseudogymnoascus protoplasts electroporation Antarctica Microbiology QR1-502 |
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transformation Pseudogymnoascus protoplasts electroporation Antarctica Microbiology QR1-502 Anaí Díaz Pablo Villanueva Vicente Oliva Carlos Gil-Durán Francisco Fierro Renato Chávez Inmaculada Vaca Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin |
topic_facet |
transformation Pseudogymnoascus protoplasts electroporation Antarctica Microbiology QR1-502 |
description |
Cold-adapted fungi isolated from Antarctica, in particular those belonging to the genus Pseudogymnoascus, are producers of secondary metabolites with interesting bioactive properties as well as enzymes with potential biotechnological applications. However, at genetic level, the study of these fungi has been hindered by the lack of suitable genetic tools such as transformation systems. In fungi, the availability of transformation systems is a key to address the functional analysis of genes related with the production of a particular metabolite or enzyme. To the best of our knowledge, the transformation of Pseudogymnoascus strains of Antarctic origin has not been achieved yet. In this work, we describe for the first time the successful transformation of a Pseudogymnoascus verrucosus strain of Antarctic origin, using two methodologies: the polyethylene glycol (PEG)-mediated transformation, and the electroporation of germinated conidia. We achieved transformation efficiencies of 15.87 ± 5.16 transformants per μg of DNA and 2.67 ± 1.15 transformants per μg of DNA for PEG-mediated transformation and electroporation of germinated conidia, respectively. These results indicate that PEG-mediated transformation is a very efficient method for the transformation of this Antarctic fungus. The genetic transformation of Pseudogymnoascus verrucosus described in this work represents the first example of transformation of a filamentous fungus of Antarctic origin. |
format |
Article in Journal/Newspaper |
author |
Anaí Díaz Pablo Villanueva Vicente Oliva Carlos Gil-Durán Francisco Fierro Renato Chávez Inmaculada Vaca |
author_facet |
Anaí Díaz Pablo Villanueva Vicente Oliva Carlos Gil-Durán Francisco Fierro Renato Chávez Inmaculada Vaca |
author_sort |
Anaí Díaz |
title |
Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin |
title_short |
Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin |
title_full |
Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin |
title_fullStr |
Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin |
title_full_unstemmed |
Genetic Transformation of the Filamentous Fungus Pseudogymnoascus verrucosus of Antarctic Origin |
title_sort |
genetic transformation of the filamentous fungus pseudogymnoascus verrucosus of antarctic origin |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fmicb.2019.02675 https://doaj.org/article/0a62c14439c8436daedf2ed62e0bf37c |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Frontiers in Microbiology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2019.02675/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.02675 https://doaj.org/article/0a62c14439c8436daedf2ed62e0bf37c |
op_doi |
https://doi.org/10.3389/fmicb.2019.02675 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
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1766046883172581376 |