Serological screening of the Schistosoma mansoni adult worm proteome.
BACKGROUND: New interventions tools are a priority for schistosomiasis control and elimination, as the disease is still highly prevalent. The identification of proteins associated with active infection and protective immune response may constitute the basis for the development of a successful vaccin...
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ftdoajarticles:oai:doaj.org/article:0a5accbb14c947e5ab75cdc433e15aad 2023-05-15T15:10:07+02:00 Serological screening of the Schistosoma mansoni adult worm proteome. Fernanda Ludolf Paola R Patrocínio Rodrigo Corrêa-Oliveira Andréa Gazzinelli Franco H Falcone André Teixeira-Ferreira Jonas Perales Guilherme C Oliveira Rosiane A Silva-Pereira 2014-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002745 https://doaj.org/article/0a5accbb14c947e5ab75cdc433e15aad EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3961189?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002745 https://doaj.org/article/0a5accbb14c947e5ab75cdc433e15aad PLoS Neglected Tropical Diseases, Vol 8, Iss 3, p e2745 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002745 2022-12-31T05:10:52Z BACKGROUND: New interventions tools are a priority for schistosomiasis control and elimination, as the disease is still highly prevalent. The identification of proteins associated with active infection and protective immune response may constitute the basis for the development of a successful vaccine and could also indicate new diagnostic candidates. In this context, post-genomic technologies have been progressing, resulting in a more rational discovery of new biomarkers of resistance and antigens for diagnosis. METHODOLOGY/PRINCIPAL FINDINGS: Two-dimensional electrophoresed Schistosoma mansoni adult worm protein extracts were probed with pooled sera of infected and non-infected (naturally resistant) individuals from a S. mansoni endemic area. A total of 47 different immunoreactive proteins were identified by mass spectrometry. Although the different pooled sera shared most of the immunoreactive protein spots, nine protein spots reacted exclusively with the serum pool of infected individuals, which correspond to annexin, major egg antigen, troponin T, filamin, disulphide-isomerase ER-60 precursor, actin and reticulocalbin. One protein spot, corresponding to eukaryotic translation elongation factor, reacted exclusively with the pooled sera of non-infected individuals living in the endemic area. Western blotting of two selected recombinant proteins, major egg antigen and hemoglobinase, showed a similar recognition pattern of that of the native protein. CONCLUDING/SIGNIFICANCE: Using a serological proteome analysis, a group of antigens related to the different infection status of the endemic area residents was identified and may be related to susceptibility or resistance to infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 3 e2745 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Fernanda Ludolf Paola R Patrocínio Rodrigo Corrêa-Oliveira Andréa Gazzinelli Franco H Falcone André Teixeira-Ferreira Jonas Perales Guilherme C Oliveira Rosiane A Silva-Pereira Serological screening of the Schistosoma mansoni adult worm proteome. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: New interventions tools are a priority for schistosomiasis control and elimination, as the disease is still highly prevalent. The identification of proteins associated with active infection and protective immune response may constitute the basis for the development of a successful vaccine and could also indicate new diagnostic candidates. In this context, post-genomic technologies have been progressing, resulting in a more rational discovery of new biomarkers of resistance and antigens for diagnosis. METHODOLOGY/PRINCIPAL FINDINGS: Two-dimensional electrophoresed Schistosoma mansoni adult worm protein extracts were probed with pooled sera of infected and non-infected (naturally resistant) individuals from a S. mansoni endemic area. A total of 47 different immunoreactive proteins were identified by mass spectrometry. Although the different pooled sera shared most of the immunoreactive protein spots, nine protein spots reacted exclusively with the serum pool of infected individuals, which correspond to annexin, major egg antigen, troponin T, filamin, disulphide-isomerase ER-60 precursor, actin and reticulocalbin. One protein spot, corresponding to eukaryotic translation elongation factor, reacted exclusively with the pooled sera of non-infected individuals living in the endemic area. Western blotting of two selected recombinant proteins, major egg antigen and hemoglobinase, showed a similar recognition pattern of that of the native protein. CONCLUDING/SIGNIFICANCE: Using a serological proteome analysis, a group of antigens related to the different infection status of the endemic area residents was identified and may be related to susceptibility or resistance to infection. |
format |
Article in Journal/Newspaper |
author |
Fernanda Ludolf Paola R Patrocínio Rodrigo Corrêa-Oliveira Andréa Gazzinelli Franco H Falcone André Teixeira-Ferreira Jonas Perales Guilherme C Oliveira Rosiane A Silva-Pereira |
author_facet |
Fernanda Ludolf Paola R Patrocínio Rodrigo Corrêa-Oliveira Andréa Gazzinelli Franco H Falcone André Teixeira-Ferreira Jonas Perales Guilherme C Oliveira Rosiane A Silva-Pereira |
author_sort |
Fernanda Ludolf |
title |
Serological screening of the Schistosoma mansoni adult worm proteome. |
title_short |
Serological screening of the Schistosoma mansoni adult worm proteome. |
title_full |
Serological screening of the Schistosoma mansoni adult worm proteome. |
title_fullStr |
Serological screening of the Schistosoma mansoni adult worm proteome. |
title_full_unstemmed |
Serological screening of the Schistosoma mansoni adult worm proteome. |
title_sort |
serological screening of the schistosoma mansoni adult worm proteome. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002745 https://doaj.org/article/0a5accbb14c947e5ab75cdc433e15aad |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 3, p e2745 (2014) |
op_relation |
http://europepmc.org/articles/PMC3961189?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002745 https://doaj.org/article/0a5accbb14c947e5ab75cdc433e15aad |
op_doi |
https://doi.org/10.1371/journal.pntd.0002745 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
3 |
container_start_page |
e2745 |
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1766341179620720640 |