Aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast Comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico
The surface of human syncytiotrophoblast does not induce maternal blood platelet aggregation even though it is not an endothelium. It can be surmised that as occurs in endothelial injury the subcellular components of the syncytiotrophoblast may have pro-or antiaggregatory activity. During congenital...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
1993
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0036-46651993000300006 https://doaj.org/article/0a3410a1f1b2440ba1887a070aa8c55c |
id |
ftdoajarticles:oai:doaj.org/article:0a3410a1f1b2440ba1887a070aa8c55c |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0a3410a1f1b2440ba1887a070aa8c55c 2024-09-09T19:27:40+00:00 Aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast Comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico A.R. Eynard S. Muñoz L. Ruiz Moreno M.E. Pasqualini S.P. de Fabro 1993-06-01T00:00:00Z https://doi.org/10.1590/S0036-46651993000300006 https://doaj.org/article/0a3410a1f1b2440ba1887a070aa8c55c EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651993000300006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651993000300006 0036-4665 1678-9946 https://doaj.org/article/0a3410a1f1b2440ba1887a070aa8c55c Revista do Instituto de Medicina Tropical de São Paulo, Vol 35, Iss 3, Pp 253-257 (1993) Human congenital Chagas' disease Human platelet aggregation Trypanosoma cruzi Chagas' disease Placenta Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1993 ftdoajarticles https://doi.org/10.1590/S0036-46651993000300006 2024-08-05T17:49:30Z The surface of human syncytiotrophoblast does not induce maternal blood platelet aggregation even though it is not an endothelium. It can be surmised that as occurs in endothelial injury the subcellular components of the syncytiotrophoblast may have pro-or antiaggregatory activity. During congenital Chagas' disease which is associated to trophoblast lesions, platelets may play a role in the development of T. cruzi-induced placentitis. In the present work the aggregatory behaviour of normal human blood platelets was recorded after their challenging with subcellular fractions of syncytiotrophoblast isolated from normal and chagasic women. Nuclear, Mitochondrial, Microsomal and Supernatant fractions isolated from normal and chagasic syncytiotrophoblast failed to induce per se any aggregatory reaction on platelets. When samples of platelet-rich plasma (PRP) were preincubated with normal and chagasic nuclear fractions and then stimulated with collagen at threshold level (CT-PRP) an inhibition of the aggregatory response was observed. Treatment of CT-PRP with normal and chagasic mitochondrial fractions induced inhibition of platelet aggregation whereas only chagasic fraction reduced latency time. Microsornal fraction from normal placentas showed no significant effects on platelet aggregation. It is concluded that subcellular fractions of normal human syncytiotrophoblast do not exhibit any effect on platelet aggregation, whereas those subcellular fractions enriched in intracellular membrane components isolated from chagasic placentas inhibit platelet aggregation. A superficie do sinciciotrofoblasto humano não induz agregação das plaquetas maternas apesar de não ser um endotélio. Lesões endoteliais propiciam o aparecimento de agregados plaquetários, o que nos leva a questionar se os componentes subcelulares do sinciciotrofoblasto também poderiam propiciar eventos semelhantes. Na doença de Chagas congênita, que está associada a lesões a nivel de trofoblasto, as plaquetas poderiam desempenhar algum papel no ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 35 3 253 257 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Human congenital Chagas' disease Human platelet aggregation Trypanosoma cruzi Chagas' disease Placenta Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Human congenital Chagas' disease Human platelet aggregation Trypanosoma cruzi Chagas' disease Placenta Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 A.R. Eynard S. Muñoz L. Ruiz Moreno M.E. Pasqualini S.P. de Fabro Aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast Comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico |
topic_facet |
Human congenital Chagas' disease Human platelet aggregation Trypanosoma cruzi Chagas' disease Placenta Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The surface of human syncytiotrophoblast does not induce maternal blood platelet aggregation even though it is not an endothelium. It can be surmised that as occurs in endothelial injury the subcellular components of the syncytiotrophoblast may have pro-or antiaggregatory activity. During congenital Chagas' disease which is associated to trophoblast lesions, platelets may play a role in the development of T. cruzi-induced placentitis. In the present work the aggregatory behaviour of normal human blood platelets was recorded after their challenging with subcellular fractions of syncytiotrophoblast isolated from normal and chagasic women. Nuclear, Mitochondrial, Microsomal and Supernatant fractions isolated from normal and chagasic syncytiotrophoblast failed to induce per se any aggregatory reaction on platelets. When samples of platelet-rich plasma (PRP) were preincubated with normal and chagasic nuclear fractions and then stimulated with collagen at threshold level (CT-PRP) an inhibition of the aggregatory response was observed. Treatment of CT-PRP with normal and chagasic mitochondrial fractions induced inhibition of platelet aggregation whereas only chagasic fraction reduced latency time. Microsornal fraction from normal placentas showed no significant effects on platelet aggregation. It is concluded that subcellular fractions of normal human syncytiotrophoblast do not exhibit any effect on platelet aggregation, whereas those subcellular fractions enriched in intracellular membrane components isolated from chagasic placentas inhibit platelet aggregation. A superficie do sinciciotrofoblasto humano não induz agregação das plaquetas maternas apesar de não ser um endotélio. Lesões endoteliais propiciam o aparecimento de agregados plaquetários, o que nos leva a questionar se os componentes subcelulares do sinciciotrofoblasto também poderiam propiciar eventos semelhantes. Na doença de Chagas congênita, que está associada a lesões a nivel de trofoblasto, as plaquetas poderiam desempenhar algum papel no ... |
format |
Article in Journal/Newspaper |
author |
A.R. Eynard S. Muñoz L. Ruiz Moreno M.E. Pasqualini S.P. de Fabro |
author_facet |
A.R. Eynard S. Muñoz L. Ruiz Moreno M.E. Pasqualini S.P. de Fabro |
author_sort |
A.R. Eynard |
title |
Aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast Comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico |
title_short |
Aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast Comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico |
title_full |
Aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast Comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico |
title_fullStr |
Aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast Comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico |
title_full_unstemmed |
Aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast Comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico |
title_sort |
aggregatory behaviour of platelets incubated with subcellular fractions of normal and chagasic human syncytiotrophoblast comportamento agregatório das plaquetas incubadas com frações subcelulares de sinciciotrofoblasto humano normal e chagásico |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1993 |
url |
https://doi.org/10.1590/S0036-46651993000300006 https://doaj.org/article/0a3410a1f1b2440ba1887a070aa8c55c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 35, Iss 3, Pp 253-257 (1993) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651993000300006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651993000300006 0036-4665 1678-9946 https://doaj.org/article/0a3410a1f1b2440ba1887a070aa8c55c |
op_doi |
https://doi.org/10.1590/S0036-46651993000300006 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
35 |
container_issue |
3 |
container_start_page |
253 |
op_container_end_page |
257 |
_version_ |
1809897069153878016 |