Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors
Microalgae with a high content of the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are of great demand for microalgae-based technologies. An Arctic strain of the diatom Attheya septentrionalis was shown in previous experiments to increase i...
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ftdoajarticles:oai:doaj.org/article:09e6114ce2364bd399a978f50aa51e47 2023-05-15T14:51:36+02:00 Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors Pia Steinrücken Svein A. Mjøs Siv K. Prestegard Svein R. Erga 2018-04-01T00:00:00Z https://doi.org/10.3389/fpls.2018.00491 https://doaj.org/article/09e6114ce2364bd399a978f50aa51e47 EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fpls.2018.00491/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2018.00491 https://doaj.org/article/09e6114ce2364bd399a978f50aa51e47 Frontiers in Plant Science, Vol 9 (2018) eicosapentaenoic acid (EPA) arctic diatom factorial design salinity growth phase interactive effects Plant culture SB1-1110 article 2018 ftdoajarticles https://doi.org/10.3389/fpls.2018.00491 2022-12-31T03:46:01Z Microalgae with a high content of the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are of great demand for microalgae-based technologies. An Arctic strain of the diatom Attheya septentrionalis was shown in previous experiments to increase its EPA content from 3.0 to 4.6% of dry weight (DW) in the nutrient-replete exponential phase and nutrient-depleted stationary phase, respectively. In the present study, a factorial-design experiment was used, to investigate this effect in more detail and in combination with varying salinities and irradiances. A mathematical model revealed that both growth phase and salinity, alone and in combination, influenced the EPA content significantly. Maximum EPA values of 7.1% DW were obtained at a salinity of 22 and after 5 days in stationary phase, and might be related to a decreased silica content, an accumulation of storage lipids containing EPA, or both. However, growth rates were lower for low salinity (0.54 and 0.57 d−1) than high salinity (0.77 and 0.98 d−1) cultures. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Frontiers in Plant Science 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
eicosapentaenoic acid (EPA) arctic diatom factorial design salinity growth phase interactive effects Plant culture SB1-1110 |
spellingShingle |
eicosapentaenoic acid (EPA) arctic diatom factorial design salinity growth phase interactive effects Plant culture SB1-1110 Pia Steinrücken Svein A. Mjøs Siv K. Prestegard Svein R. Erga Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
topic_facet |
eicosapentaenoic acid (EPA) arctic diatom factorial design salinity growth phase interactive effects Plant culture SB1-1110 |
description |
Microalgae with a high content of the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are of great demand for microalgae-based technologies. An Arctic strain of the diatom Attheya septentrionalis was shown in previous experiments to increase its EPA content from 3.0 to 4.6% of dry weight (DW) in the nutrient-replete exponential phase and nutrient-depleted stationary phase, respectively. In the present study, a factorial-design experiment was used, to investigate this effect in more detail and in combination with varying salinities and irradiances. A mathematical model revealed that both growth phase and salinity, alone and in combination, influenced the EPA content significantly. Maximum EPA values of 7.1% DW were obtained at a salinity of 22 and after 5 days in stationary phase, and might be related to a decreased silica content, an accumulation of storage lipids containing EPA, or both. However, growth rates were lower for low salinity (0.54 and 0.57 d−1) than high salinity (0.77 and 0.98 d−1) cultures. |
format |
Article in Journal/Newspaper |
author |
Pia Steinrücken Svein A. Mjøs Siv K. Prestegard Svein R. Erga |
author_facet |
Pia Steinrücken Svein A. Mjøs Siv K. Prestegard Svein R. Erga |
author_sort |
Pia Steinrücken |
title |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_short |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_full |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_fullStr |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_full_unstemmed |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_sort |
enhancing epa content in an arctic diatom: a factorial design study to evaluate interactive effects of growth factors |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
https://doi.org/10.3389/fpls.2018.00491 https://doaj.org/article/09e6114ce2364bd399a978f50aa51e47 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Frontiers in Plant Science, Vol 9 (2018) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fpls.2018.00491/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2018.00491 https://doaj.org/article/09e6114ce2364bd399a978f50aa51e47 |
op_doi |
https://doi.org/10.3389/fpls.2018.00491 |
container_title |
Frontiers in Plant Science |
container_volume |
9 |
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1766322739736477696 |