Lipase B from Candida antarctica Immobilized on a Silica-Lignin Matrix as a Stable and Reusable Biocatalytic System
A study was conducted of the possible use of a silica-lignin hybrid as a novel support for the immobilization of lipase B from Candida antarctica. Results obtained by elemental analysis, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and atomic force microsco...
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ftdoajarticles:oai:doaj.org/article:09dfd68236a54ed1ae652280d6f54dd8 2024-10-13T14:03:10+00:00 Lipase B from Candida antarctica Immobilized on a Silica-Lignin Matrix as a Stable and Reusable Biocatalytic System Jakub Zdarta Lukasz Klapiszewski Artur Jedrzak Marek Nowicki Dariusz Moszynski Teofil Jesionowski 2016-12-01T00:00:00Z https://doi.org/10.3390/catal7010014 https://doaj.org/article/09dfd68236a54ed1ae652280d6f54dd8 EN eng MDPI AG http://www.mdpi.com/2073-4344/7/1/14 https://doaj.org/toc/2073-4344 doi:10.3390/catal7010014 https://doaj.org/article/09dfd68236a54ed1ae652280d6f54dd8 Catalysts, Vol 7, Iss 1, p 14 (2016) silica-lignin matrix lipase immobilized enzymes enzyme activity and stability Chemical technology TP1-1185 Chemistry QD1-999 article 2016 ftdoajarticles https://doi.org/10.3390/catal7010014 2024-09-25T15:39:10Z A study was conducted of the possible use of a silica-lignin hybrid as a novel support for the immobilization of lipase B from Candida antarctica. Results obtained by elemental analysis, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM), as well as the determination of changes in porous structure parameters, confirmed the effective immobilization of the enzyme on the surface of the composite matrix. Based on a hydrolysis reaction, a determination was made of the retention of activity of the immobilized lipase, found to be 92% of that of the native enzyme. Immobilization on a silica-lignin matrix produces systems with maximum activity at pH = 8 and at a temperature of 40 °C. The immobilized enzyme exhibited increased thermal and chemical stability and retained more than 80% of its activity after 20 reaction cycles. Moreover immobilized lipase exhibited over 80% of its activity at pH range 7–9 and temperature from 30 °C to 60 °C, while native Candida antarctica lipase B (CALB) exhibited the same only at pH = 7 and temperature of 30 °C. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Catalysts 7 12 14 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
silica-lignin matrix lipase immobilized enzymes enzyme activity and stability Chemical technology TP1-1185 Chemistry QD1-999 |
spellingShingle |
silica-lignin matrix lipase immobilized enzymes enzyme activity and stability Chemical technology TP1-1185 Chemistry QD1-999 Jakub Zdarta Lukasz Klapiszewski Artur Jedrzak Marek Nowicki Dariusz Moszynski Teofil Jesionowski Lipase B from Candida antarctica Immobilized on a Silica-Lignin Matrix as a Stable and Reusable Biocatalytic System |
topic_facet |
silica-lignin matrix lipase immobilized enzymes enzyme activity and stability Chemical technology TP1-1185 Chemistry QD1-999 |
description |
A study was conducted of the possible use of a silica-lignin hybrid as a novel support for the immobilization of lipase B from Candida antarctica. Results obtained by elemental analysis, Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM), as well as the determination of changes in porous structure parameters, confirmed the effective immobilization of the enzyme on the surface of the composite matrix. Based on a hydrolysis reaction, a determination was made of the retention of activity of the immobilized lipase, found to be 92% of that of the native enzyme. Immobilization on a silica-lignin matrix produces systems with maximum activity at pH = 8 and at a temperature of 40 °C. The immobilized enzyme exhibited increased thermal and chemical stability and retained more than 80% of its activity after 20 reaction cycles. Moreover immobilized lipase exhibited over 80% of its activity at pH range 7–9 and temperature from 30 °C to 60 °C, while native Candida antarctica lipase B (CALB) exhibited the same only at pH = 7 and temperature of 30 °C. |
format |
Article in Journal/Newspaper |
author |
Jakub Zdarta Lukasz Klapiszewski Artur Jedrzak Marek Nowicki Dariusz Moszynski Teofil Jesionowski |
author_facet |
Jakub Zdarta Lukasz Klapiszewski Artur Jedrzak Marek Nowicki Dariusz Moszynski Teofil Jesionowski |
author_sort |
Jakub Zdarta |
title |
Lipase B from Candida antarctica Immobilized on a Silica-Lignin Matrix as a Stable and Reusable Biocatalytic System |
title_short |
Lipase B from Candida antarctica Immobilized on a Silica-Lignin Matrix as a Stable and Reusable Biocatalytic System |
title_full |
Lipase B from Candida antarctica Immobilized on a Silica-Lignin Matrix as a Stable and Reusable Biocatalytic System |
title_fullStr |
Lipase B from Candida antarctica Immobilized on a Silica-Lignin Matrix as a Stable and Reusable Biocatalytic System |
title_full_unstemmed |
Lipase B from Candida antarctica Immobilized on a Silica-Lignin Matrix as a Stable and Reusable Biocatalytic System |
title_sort |
lipase b from candida antarctica immobilized on a silica-lignin matrix as a stable and reusable biocatalytic system |
publisher |
MDPI AG |
publishDate |
2016 |
url |
https://doi.org/10.3390/catal7010014 https://doaj.org/article/09dfd68236a54ed1ae652280d6f54dd8 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts, Vol 7, Iss 1, p 14 (2016) |
op_relation |
http://www.mdpi.com/2073-4344/7/1/14 https://doaj.org/toc/2073-4344 doi:10.3390/catal7010014 https://doaj.org/article/09dfd68236a54ed1ae652280d6f54dd8 |
op_doi |
https://doi.org/10.3390/catal7010014 |
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Catalysts |
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7 |
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12 |
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14 |
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