Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum
Objective: To investigate the role of toll-like receptor 2 (TLR2) in inflammatory activity of macrophage infected with the recombinant Mycobacterium bovis bacillus Calmette-Guerin (rBCG). Methods: Mouse macrophage cell line J774A.1 was infected with Mycobacterium bovis bacillus Calmette-Guerin (BCG)...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2018
|
Subjects: | |
Online Access: | https://doi.org/10.4103/2221-1691.237075 https://doaj.org/article/09de0349b4c74829a665291fe81254c0 |
id |
ftdoajarticles:oai:doaj.org/article:09de0349b4c74829a665291fe81254c0 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:09de0349b4c74829a665291fe81254c0 2023-05-15T15:11:55+02:00 Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum Nor Munirah Zakaria Rapeah Suppian Norazmi Mohd Nor Nor Fazila Che Mat 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.237075 https://doaj.org/article/09de0349b4c74829a665291fe81254c0 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=7;spage=333;epage=339;aulast=Zakaria https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.237075 https://doaj.org/article/09de0349b4c74829a665291fe81254c0 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 7, Pp 333-339 (2018) macrophages mycobacterium bovis bacillus calmette-guerin plasmodium falciparum proinflammatory cytokine toll-like receptor 2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.237075 2022-12-30T22:57:18Z Objective: To investigate the role of toll-like receptor 2 (TLR2) in inflammatory activity of macrophage infected with the recombinant Mycobacterium bovis bacillus Calmette-Guerin (rBCG). Methods: Mouse macrophage cell line J774A.1 was infected with Mycobacterium bovis bacillus Calmette-Guerin (BCG) and rBCG cultures for 48 h in the presence or absence of 10 μg/mL of TLR2 inhibitor. Untreated macrophages were used as a negative control while lipopolysaccharide-stimulated macrophages were used as a positive control. The ability of the macrophage to engulf the BCG and rBCG in the absence or presence of TLR2 inhibitor was assessed using a phagocytic assay, while the production of inflammatory cytokines and nitric oxide by the infected macrophages was evaluated using ELISA and Griess reagent method, while the expression of the inducible nitric oxide synthase was determined using Western blot analysis. Results: The results showed that blocking TLR2 function reduced the phagocytic activity, nitric oxide production and proinflammatory cytokine secretion such as TNF- α, IL-1 β and IL-12p40 as well as inducible nitric oxide synthase expression in the infected macrophages. These data showed the importance of TLR2 in the activation of macrophages following BCG and rBCG infections. Conclusions: Through exploring the immunological mechanism which underlies the protection conferred by the candidate vaccine, this study will improve our understanding of the vaccine candidate’s mechanism to protect the host from malaria infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Calmette ENVELOPE(-67.150,-67.150,-68.050,-68.050) Asian Pacific Journal of Tropical Biomedicine 8 7 333 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
macrophages mycobacterium bovis bacillus calmette-guerin plasmodium falciparum proinflammatory cytokine toll-like receptor 2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
macrophages mycobacterium bovis bacillus calmette-guerin plasmodium falciparum proinflammatory cytokine toll-like receptor 2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nor Munirah Zakaria Rapeah Suppian Norazmi Mohd Nor Nor Fazila Che Mat Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum |
topic_facet |
macrophages mycobacterium bovis bacillus calmette-guerin plasmodium falciparum proinflammatory cytokine toll-like receptor 2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the role of toll-like receptor 2 (TLR2) in inflammatory activity of macrophage infected with the recombinant Mycobacterium bovis bacillus Calmette-Guerin (rBCG). Methods: Mouse macrophage cell line J774A.1 was infected with Mycobacterium bovis bacillus Calmette-Guerin (BCG) and rBCG cultures for 48 h in the presence or absence of 10 μg/mL of TLR2 inhibitor. Untreated macrophages were used as a negative control while lipopolysaccharide-stimulated macrophages were used as a positive control. The ability of the macrophage to engulf the BCG and rBCG in the absence or presence of TLR2 inhibitor was assessed using a phagocytic assay, while the production of inflammatory cytokines and nitric oxide by the infected macrophages was evaluated using ELISA and Griess reagent method, while the expression of the inducible nitric oxide synthase was determined using Western blot analysis. Results: The results showed that blocking TLR2 function reduced the phagocytic activity, nitric oxide production and proinflammatory cytokine secretion such as TNF- α, IL-1 β and IL-12p40 as well as inducible nitric oxide synthase expression in the infected macrophages. These data showed the importance of TLR2 in the activation of macrophages following BCG and rBCG infections. Conclusions: Through exploring the immunological mechanism which underlies the protection conferred by the candidate vaccine, this study will improve our understanding of the vaccine candidate’s mechanism to protect the host from malaria infection. |
format |
Article in Journal/Newspaper |
author |
Nor Munirah Zakaria Rapeah Suppian Norazmi Mohd Nor Nor Fazila Che Mat |
author_facet |
Nor Munirah Zakaria Rapeah Suppian Norazmi Mohd Nor Nor Fazila Che Mat |
author_sort |
Nor Munirah Zakaria |
title |
Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum |
title_short |
Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum |
title_full |
Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum |
title_fullStr |
Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum |
title_full_unstemmed |
Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum |
title_sort |
role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant bcg expressing the c-terminus of merozoite surface protein-1 of plasmodium falciparum |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.237075 https://doaj.org/article/09de0349b4c74829a665291fe81254c0 |
long_lat |
ENVELOPE(-67.150,-67.150,-68.050,-68.050) |
geographic |
Arctic Calmette |
geographic_facet |
Arctic Calmette |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 7, Pp 333-339 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=7;spage=333;epage=339;aulast=Zakaria https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.237075 https://doaj.org/article/09de0349b4c74829a665291fe81254c0 |
op_doi |
https://doi.org/10.4103/2221-1691.237075 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
7 |
container_start_page |
333 |
_version_ |
1766342689401339904 |