Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt
The present study aimed to describe F. pedrosoi propagules capable of causing chronic murine disease. Several changes in F. pedrosoi hyphae were identified in fungal cells cultured for a long period. Optical microscopy found many rounded cells with double-rigid melanin-rich walls. Terminal and inter...
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ftdoajarticles:oai:doaj.org/article:09c88df6604743d1b6e1e5d3e2ed1607 2023-05-15T15:08:40+02:00 Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt AP Machado E Freymuller O Fischman 2009-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992009000400007 https://doaj.org/article/09c88df6604743d1b6e1e5d3e2ed1607 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400007 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000400007 1678-9199 https://doaj.org/article/09c88df6604743d1b6e1e5d3e2ed1607 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 4, Pp 680-695 (2009) Fonsecaea pedrosoi experimental chromoblastomycosis sclerotic cells Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2009 ftdoajarticles https://doi.org/10.1590/S1678-91992009000400007 2022-12-31T00:08:46Z The present study aimed to describe F. pedrosoi propagules capable of causing chronic murine disease. Several changes in F. pedrosoi hyphae were identified in fungal cells cultured for a long period. Optical microscopy found many rounded cells with double-rigid melanin-rich walls. Terminal and intercalary chlamydoconidia were also frequently observed. Analyses of images from transmission electron microscopy (TEM) revealed several cells with walls composed of at least three layers and an outer layer enriched with melanin. Two groups of twenty BALB/c mice were subcutaneously infected in their footpads with F. pedrosoi cells at an inoculum concentration of approximately 1 x 10(4) cells/mL. In one group, long-term cultured F. pedrosoi cells were inoculated in one footpad, whereas in the other group, both footpads were infected. Active lesions were observed up to seven months post-infection, particularly in mice inoculated at two sites. After this period, animals were killed. Histological sections revealed characteristics bearing a strong resemblance to the human form of the disease such as tissue hyperplasia, granulomas with microabscesses and sclerotic cells. Based on this study, we identified fungal cells from old cultures capable of provoking chronic chromoblastomycosis under experimental conditions, especially when more than one site is infected. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 15 4 680 695 |
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Directory of Open Access Journals: DOAJ Articles |
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Fonsecaea pedrosoi experimental chromoblastomycosis sclerotic cells Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Fonsecaea pedrosoi experimental chromoblastomycosis sclerotic cells Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 AP Machado E Freymuller O Fischman Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt |
topic_facet |
Fonsecaea pedrosoi experimental chromoblastomycosis sclerotic cells Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The present study aimed to describe F. pedrosoi propagules capable of causing chronic murine disease. Several changes in F. pedrosoi hyphae were identified in fungal cells cultured for a long period. Optical microscopy found many rounded cells with double-rigid melanin-rich walls. Terminal and intercalary chlamydoconidia were also frequently observed. Analyses of images from transmission electron microscopy (TEM) revealed several cells with walls composed of at least three layers and an outer layer enriched with melanin. Two groups of twenty BALB/c mice were subcutaneously infected in their footpads with F. pedrosoi cells at an inoculum concentration of approximately 1 x 10(4) cells/mL. In one group, long-term cultured F. pedrosoi cells were inoculated in one footpad, whereas in the other group, both footpads were infected. Active lesions were observed up to seven months post-infection, particularly in mice inoculated at two sites. After this period, animals were killed. Histological sections revealed characteristics bearing a strong resemblance to the human form of the disease such as tissue hyperplasia, granulomas with microabscesses and sclerotic cells. Based on this study, we identified fungal cells from old cultures capable of provoking chronic chromoblastomycosis under experimental conditions, especially when more than one site is infected. |
format |
Article in Journal/Newspaper |
author |
AP Machado E Freymuller O Fischman |
author_facet |
AP Machado E Freymuller O Fischman |
author_sort |
AP Machado |
title |
Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt |
title_short |
Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt |
title_full |
Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt |
title_fullStr |
Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt |
title_full_unstemmed |
Experimental murine chromoblastomycosis obtained from Fonsecaea pedrosoi isolate cultured for a long periodt |
title_sort |
experimental murine chromoblastomycosis obtained from fonsecaea pedrosoi isolate cultured for a long periodt |
publisher |
SciELO |
publishDate |
2009 |
url |
https://doi.org/10.1590/S1678-91992009000400007 https://doaj.org/article/09c88df6604743d1b6e1e5d3e2ed1607 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 4, Pp 680-695 (2009) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400007 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000400007 1678-9199 https://doaj.org/article/09c88df6604743d1b6e1e5d3e2ed1607 |
op_doi |
https://doi.org/10.1590/S1678-91992009000400007 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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15 |
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