Identification of Cryptococcus neoformans isolates using Staib agar without creatinine
Abstract: In mycology, culture is the best way to demonstrate and identify pathogenic fungi. Many kinds of media have been developed and modified, using fungal biochemical properties to recognize them. The most common media are Sabouraud, Malt Agar, Cornmeal agar, Potato-dextrose agar, and Staib aga...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2005
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ftdoajarticles:oai:doaj.org/article:0974a7c2ee8e4d83b829cc46f47cfa79 2023-10-01T03:54:19+02:00 Identification of Cryptococcus neoformans isolates using Staib agar without creatinine Nardelli Vanessa Pérez Celina Mata-Essayag Sofía Colella María Teresa Roselló Arantza Hartung de Capriles Claudia Landaeta María Eugenia Olaizola Carolina Magaldi, Sylvia 2005-12-01T00:00:00Z https://doaj.org/article/0974a7c2ee8e4d83b829cc46f47cfa79 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://drive.google.com/file/d/1jOTd3Csc12oAPz60VsJy3P-jtKApWWJM/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/0974a7c2ee8e4d83b829cc46f47cfa79 Kasmera, Vol 33, Iss 2, Pp 102-108 (2005) Staib agar Guizotia abyssinica Cryptococcus neoformans creatinine Agar Staib creatinina Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2005 ftdoajarticles 2023-09-03T00:45:41Z Abstract: In mycology, culture is the best way to demonstrate and identify pathogenic fungi. Many kinds of media have been developed and modified, using fungal biochemical properties to recognize them. The most common media are Sabouraud, Malt Agar, Cornmeal agar, Potato-dextrose agar, and Staib agar. Staib agar has been widely used for identification of yeasts from the genus Cryptococcus and other fungi. The aim of this study was to demonstrate the usefulness of Staib agar, and of a modified Staib medium without creatinine, in the isolation and identification of fungi. Forty-six Cryptococcus neoformans strains plus one control strain was identified by morphological and biochemical criteria, using urease test (Christensen urea agar) and culture in Sablac medium. The strains were then transferred into standard Staib and Staib without creatinine agar plates. Morphological features and phenoloxidase production were evaluated after one-week incubation at room temperature (26-28°C). Culture in standard and modified Staib agar yielded brown color colonies (phenoloxidase production) in both media in 43 (91.5%) cases. Staib agar without creatinine is an excellent medium for identification of C. neoformans, with the advantage of a low cost and easy preparation. Resumen: En micología, el cultivo es la mejor manera de realizar el diagnóstico definitivo de una micosis. Muchos tipos de medios han sido desarrollados y modificados para tal fin, utilizando las propiedades bioquímicas de los hongos para reconocerlos. Los medios más comunes son Sabouraud, agar Malta, agar maíz, agar Papa-dextrosa, y agar Staib. Este último ha sido ampliamente usado para la identificación de levaduras del género Cryptococcus y en otros hongos. El objetivo de este estudio fue demostrar la utilidad del agar Staib y de un medio de Staib modificado, sin creatinina, en el aislamiento e identificación de hongos patógenos. Cuarenta y seis aislados de Cryptococcus neoformans más un control fueron identificados por criterios morfológicos y bioquímicos, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Christensen ENVELOPE(47.867,47.867,-67.967,-67.967) |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Staib agar Guizotia abyssinica Cryptococcus neoformans creatinine Agar Staib creatinina Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Staib agar Guizotia abyssinica Cryptococcus neoformans creatinine Agar Staib creatinina Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nardelli Vanessa Pérez Celina Mata-Essayag Sofía Colella María Teresa Roselló Arantza Hartung de Capriles Claudia Landaeta María Eugenia Olaizola Carolina Magaldi, Sylvia Identification of Cryptococcus neoformans isolates using Staib agar without creatinine |
topic_facet |
Staib agar Guizotia abyssinica Cryptococcus neoformans creatinine Agar Staib creatinina Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract: In mycology, culture is the best way to demonstrate and identify pathogenic fungi. Many kinds of media have been developed and modified, using fungal biochemical properties to recognize them. The most common media are Sabouraud, Malt Agar, Cornmeal agar, Potato-dextrose agar, and Staib agar. Staib agar has been widely used for identification of yeasts from the genus Cryptococcus and other fungi. The aim of this study was to demonstrate the usefulness of Staib agar, and of a modified Staib medium without creatinine, in the isolation and identification of fungi. Forty-six Cryptococcus neoformans strains plus one control strain was identified by morphological and biochemical criteria, using urease test (Christensen urea agar) and culture in Sablac medium. The strains were then transferred into standard Staib and Staib without creatinine agar plates. Morphological features and phenoloxidase production were evaluated after one-week incubation at room temperature (26-28°C). Culture in standard and modified Staib agar yielded brown color colonies (phenoloxidase production) in both media in 43 (91.5%) cases. Staib agar without creatinine is an excellent medium for identification of C. neoformans, with the advantage of a low cost and easy preparation. Resumen: En micología, el cultivo es la mejor manera de realizar el diagnóstico definitivo de una micosis. Muchos tipos de medios han sido desarrollados y modificados para tal fin, utilizando las propiedades bioquímicas de los hongos para reconocerlos. Los medios más comunes son Sabouraud, agar Malta, agar maíz, agar Papa-dextrosa, y agar Staib. Este último ha sido ampliamente usado para la identificación de levaduras del género Cryptococcus y en otros hongos. El objetivo de este estudio fue demostrar la utilidad del agar Staib y de un medio de Staib modificado, sin creatinina, en el aislamiento e identificación de hongos patógenos. Cuarenta y seis aislados de Cryptococcus neoformans más un control fueron identificados por criterios morfológicos y bioquímicos, ... |
format |
Article in Journal/Newspaper |
author |
Nardelli Vanessa Pérez Celina Mata-Essayag Sofía Colella María Teresa Roselló Arantza Hartung de Capriles Claudia Landaeta María Eugenia Olaizola Carolina Magaldi, Sylvia |
author_facet |
Nardelli Vanessa Pérez Celina Mata-Essayag Sofía Colella María Teresa Roselló Arantza Hartung de Capriles Claudia Landaeta María Eugenia Olaizola Carolina Magaldi, Sylvia |
author_sort |
Nardelli Vanessa |
title |
Identification of Cryptococcus neoformans isolates using Staib agar without creatinine |
title_short |
Identification of Cryptococcus neoformans isolates using Staib agar without creatinine |
title_full |
Identification of Cryptococcus neoformans isolates using Staib agar without creatinine |
title_fullStr |
Identification of Cryptococcus neoformans isolates using Staib agar without creatinine |
title_full_unstemmed |
Identification of Cryptococcus neoformans isolates using Staib agar without creatinine |
title_sort |
identification of cryptococcus neoformans isolates using staib agar without creatinine |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2005 |
url |
https://doaj.org/article/0974a7c2ee8e4d83b829cc46f47cfa79 |
long_lat |
ENVELOPE(47.867,47.867,-67.967,-67.967) |
geographic |
Arctic Christensen |
geographic_facet |
Arctic Christensen |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 33, Iss 2, Pp 102-108 (2005) |
op_relation |
https://drive.google.com/file/d/1jOTd3Csc12oAPz60VsJy3P-jtKApWWJM/view https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/0974a7c2ee8e4d83b829cc46f47cfa79 |
_version_ |
1778521819662254080 |