Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro
Objective: To evaluate the anti-microsporidial effects of the active component of Nigella sativa seeds, thymoquinone, against Encephalitozoon intestinalis using an in vitro model. Methods: Anti-microsporidial effect of thymoquinone against Encephalitozoon intestinalis was evaluated by using various...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2020
|
Subjects: | |
Online Access: | https://doi.org/10.4103/2221-1691.273093 https://doaj.org/article/0949fda87e5d4eb89fc44db745f5751e |
id |
ftdoajarticles:oai:doaj.org/article:0949fda87e5d4eb89fc44db745f5751e |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:0949fda87e5d4eb89fc44db745f5751e 2023-05-15T15:04:59+02:00 Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro Ulfet Cetinkaya Gülay Sezer Arzuv Charyyeva 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.273093 https://doaj.org/article/0949fda87e5d4eb89fc44db745f5751e EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=1;spage=42;epage=46;aulast=Cetinkaya https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.273093 https://doaj.org/article/0949fda87e5d4eb89fc44db745f5751e Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 1, Pp 42-46 (2020) microsporidia thymoquinone nigella sativa anti-parasitic encephalitozoon intestinalis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.273093 2022-12-31T11:49:04Z Objective: To evaluate the anti-microsporidial effects of the active component of Nigella sativa seeds, thymoquinone, against Encephalitozoon intestinalis using an in vitro model. Methods: Anti-microsporidial effect of thymoquinone against Encephalitozoon intestinalis was evaluated by using various concentrations of thymoquinone (0, 1, 5, 10, 15, 20, 30, 35, and 40 μΜ) and sterile dimethyl sulfoxide. Real time PCR was used to evaluate the inhibitory effects of thymoquinone on the life cycle of Encephalitozoon intestinalis. Results: The cytotoxic effect of thymoquinone on HEK293 cell line was observed with 30, 35, and 40 μM concentrations of thymoquinone after 24, 48, and 72 hours of incubation. It was observed that 10, 15, 20, and 30 μM concentrations of thymoquinone decreased the spore density compared with the control; however, it was significant only at 30 μM. Conclusions: Thymoquinone shows potent anti-microsporidial effects against Encephalitozoon intestinalis in the in vitro model; however, the toxic concentrations of thymoquinone are also toxic to the host cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 1 42 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
microsporidia thymoquinone nigella sativa anti-parasitic encephalitozoon intestinalis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
microsporidia thymoquinone nigella sativa anti-parasitic encephalitozoon intestinalis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Ulfet Cetinkaya Gülay Sezer Arzuv Charyyeva Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro |
topic_facet |
microsporidia thymoquinone nigella sativa anti-parasitic encephalitozoon intestinalis Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the anti-microsporidial effects of the active component of Nigella sativa seeds, thymoquinone, against Encephalitozoon intestinalis using an in vitro model. Methods: Anti-microsporidial effect of thymoquinone against Encephalitozoon intestinalis was evaluated by using various concentrations of thymoquinone (0, 1, 5, 10, 15, 20, 30, 35, and 40 μΜ) and sterile dimethyl sulfoxide. Real time PCR was used to evaluate the inhibitory effects of thymoquinone on the life cycle of Encephalitozoon intestinalis. Results: The cytotoxic effect of thymoquinone on HEK293 cell line was observed with 30, 35, and 40 μM concentrations of thymoquinone after 24, 48, and 72 hours of incubation. It was observed that 10, 15, 20, and 30 μM concentrations of thymoquinone decreased the spore density compared with the control; however, it was significant only at 30 μM. Conclusions: Thymoquinone shows potent anti-microsporidial effects against Encephalitozoon intestinalis in the in vitro model; however, the toxic concentrations of thymoquinone are also toxic to the host cells. |
format |
Article in Journal/Newspaper |
author |
Ulfet Cetinkaya Gülay Sezer Arzuv Charyyeva |
author_facet |
Ulfet Cetinkaya Gülay Sezer Arzuv Charyyeva |
author_sort |
Ulfet Cetinkaya |
title |
Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro |
title_short |
Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro |
title_full |
Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro |
title_fullStr |
Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro |
title_full_unstemmed |
Anti-microsporidial effect of thymoquinone on Encephalitozoon intestinalis infection in vitro |
title_sort |
anti-microsporidial effect of thymoquinone on encephalitozoon intestinalis infection in vitro |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.273093 https://doaj.org/article/0949fda87e5d4eb89fc44db745f5751e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 1, Pp 42-46 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=1;spage=42;epage=46;aulast=Cetinkaya https://doaj.org/toc/2588-9222 2588-9222 doi:10.4103/2221-1691.273093 https://doaj.org/article/0949fda87e5d4eb89fc44db745f5751e |
op_doi |
https://doi.org/10.4103/2221-1691.273093 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
1 |
container_start_page |
42 |
_version_ |
1766336755503464448 |