Evaluation of Baciloscopy, Cultivation and Polimerasa Chain Reaction for the Diagnostic of Lung Tuberculosis
The utility of baciloscopy, cultivation and Polimerasa Chain Reaction of (PCR) was evaluated in 31 sputum samples, of patient of both sexes, without distinction of etnial grup and ages between 17 and 80 years, with clinical manifestations, discoveries radiological and/or suggestive epidemic data of...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2005
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ftdoajarticles:oai:doaj.org/article:0948f44f5c4c46579fb616ec16dbf860 2023-10-01T03:54:16+02:00 Evaluation of Baciloscopy, Cultivation and Polimerasa Chain Reaction for the Diagnostic of Lung Tuberculosis Orlando Nava Paz Manzur Hassanhi Lisbeth Prieto 2005-08-01T00:00:00Z https://doaj.org/article/0948f44f5c4c46579fb616ec16dbf860 EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/4757 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/0948f44f5c4c46579fb616ec16dbf860 Kasmera, Vol 33, Iss 2, Pp 119-131 (2005) Tuberculosis RCP Examen directo cultivo Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2005 ftdoajarticles 2023-09-03T00:35:22Z The utility of baciloscopy, cultivation and Polimerasa Chain Reaction of (PCR) was evaluated in 31 sputum samples, of patient of both sexes, without distinction of etnial grup and ages between 17 and 80 years, with clinical manifestations, discoveries radiological and/or suggestive epidemic data of lung tuberculosis. To all the samples they were practiced baciloscopy using the technique of Ziehl-Neelsen. The Ogawa-Kudoh cultivation was used like standard. The objectives for the rehearsal of PCR were the genes that code the protein 32-KDa (alpha antigen) and the insertion sequence IS6110. After the amplification was obtained a fragment of 984 couples of bases corresponding to the sequence of insert IS6110 and another of 506 couples corresponding to the alpha antigen. Of the total of samples examined by the Ziehl-Neelsen method, 19 were positive and 12 were negative (61.29 and 38.71% respectively). 19 samples (61.29%) were positive and 12 (38.71%) were negative to the cultivation. Of all amplified samples, 22 (70.69%) showed the presence of fragments corresponding to DNA so much to the sequence of insert IS6110 like the antigen alpha 32-KDa, while in 9 (20.04%) presence of DNA mycobacterium was not detected for RCP method. The sensibility of the RCP test was virtually 100% and its specificity was of 100%. It was demonstrated that RCP, based on the simultaneous amplification of the insertion element IS6110 and the gene that codes the alpha antigen in a single step, can identify mycobacterium infections. We conclude also that is more sensitive that routine bacteriological procedures (direct exam and cultivation) and can be a very powerful tool for the diagnosis of lung tuberculosis and other mycobacterium diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
Tuberculosis RCP Examen directo cultivo Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Tuberculosis RCP Examen directo cultivo Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Orlando Nava Paz Manzur Hassanhi Lisbeth Prieto Evaluation of Baciloscopy, Cultivation and Polimerasa Chain Reaction for the Diagnostic of Lung Tuberculosis |
topic_facet |
Tuberculosis RCP Examen directo cultivo Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The utility of baciloscopy, cultivation and Polimerasa Chain Reaction of (PCR) was evaluated in 31 sputum samples, of patient of both sexes, without distinction of etnial grup and ages between 17 and 80 years, with clinical manifestations, discoveries radiological and/or suggestive epidemic data of lung tuberculosis. To all the samples they were practiced baciloscopy using the technique of Ziehl-Neelsen. The Ogawa-Kudoh cultivation was used like standard. The objectives for the rehearsal of PCR were the genes that code the protein 32-KDa (alpha antigen) and the insertion sequence IS6110. After the amplification was obtained a fragment of 984 couples of bases corresponding to the sequence of insert IS6110 and another of 506 couples corresponding to the alpha antigen. Of the total of samples examined by the Ziehl-Neelsen method, 19 were positive and 12 were negative (61.29 and 38.71% respectively). 19 samples (61.29%) were positive and 12 (38.71%) were negative to the cultivation. Of all amplified samples, 22 (70.69%) showed the presence of fragments corresponding to DNA so much to the sequence of insert IS6110 like the antigen alpha 32-KDa, while in 9 (20.04%) presence of DNA mycobacterium was not detected for RCP method. The sensibility of the RCP test was virtually 100% and its specificity was of 100%. It was demonstrated that RCP, based on the simultaneous amplification of the insertion element IS6110 and the gene that codes the alpha antigen in a single step, can identify mycobacterium infections. We conclude also that is more sensitive that routine bacteriological procedures (direct exam and cultivation) and can be a very powerful tool for the diagnosis of lung tuberculosis and other mycobacterium diseases. |
format |
Article in Journal/Newspaper |
author |
Orlando Nava Paz Manzur Hassanhi Lisbeth Prieto |
author_facet |
Orlando Nava Paz Manzur Hassanhi Lisbeth Prieto |
author_sort |
Orlando Nava Paz |
title |
Evaluation of Baciloscopy, Cultivation and Polimerasa Chain Reaction for the Diagnostic of Lung Tuberculosis |
title_short |
Evaluation of Baciloscopy, Cultivation and Polimerasa Chain Reaction for the Diagnostic of Lung Tuberculosis |
title_full |
Evaluation of Baciloscopy, Cultivation and Polimerasa Chain Reaction for the Diagnostic of Lung Tuberculosis |
title_fullStr |
Evaluation of Baciloscopy, Cultivation and Polimerasa Chain Reaction for the Diagnostic of Lung Tuberculosis |
title_full_unstemmed |
Evaluation of Baciloscopy, Cultivation and Polimerasa Chain Reaction for the Diagnostic of Lung Tuberculosis |
title_sort |
evaluation of baciloscopy, cultivation and polimerasa chain reaction for the diagnostic of lung tuberculosis |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2005 |
url |
https://doaj.org/article/0948f44f5c4c46579fb616ec16dbf860 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 33, Iss 2, Pp 119-131 (2005) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/4757 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/0948f44f5c4c46579fb616ec16dbf860 |
_version_ |
1778521717157658624 |