Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure.
Plasmodium vivax blood-stage invasion into reticulocyte is critical for parasite development. Thus, validation of novel parasite invasion ligands is essential for malaria vaccine development. Recently, we demonstrated that EBP2, a Duffy binding protein (DBP) paralog, is antigenically distinct from D...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:093e8018a76640ec9b67fa9b7e179577 2023-05-15T15:17:05+02:00 Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. Bárbara A S Lima Gabriela M Fernandes Letícia M Torres Camilla V Pires Jéssica R S Alves Sâmick L Moreira-Nascimento Maria Fernanda A Nascimento Sofia L Afonso Helena L Costa Isabela P Cerávolo Tais N Sousa Irene S Soares Francis B Ntumngia John H Adams Luzia H Carvalho Flora S Kano 2022-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010493 https://doaj.org/article/093e8018a76640ec9b67fa9b7e179577 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010493 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010493 https://doaj.org/article/093e8018a76640ec9b67fa9b7e179577 PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010493 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010493 2023-02-26T01:41:57Z Plasmodium vivax blood-stage invasion into reticulocyte is critical for parasite development. Thus, validation of novel parasite invasion ligands is essential for malaria vaccine development. Recently, we demonstrated that EBP2, a Duffy binding protein (DBP) paralog, is antigenically distinct from DBP and could not be functionally inhibited by anti-DBP antibodies. Here, we took advantage of a small outbreak of P.vivax malaria, located in a non-malarious area of Brazil, to investigate for the first time IgM/IgG antibodies against EBP2 and DEKnull-2 (an engineering DBPII vaccine) among individuals who had their first and brief exposure to P.vivax (16 cases and 22 non-cases). Our experimental approach included 4 cross sectional surveys at 3-month interval (12-month follow-up). The results demonstrated that while a brief initial P.vivax infection was not efficient to induce IgM/ IgG antibodies to either EBP2 or DEKnull-2, IgG antibodies against DEKnull-2 (but not EBP2) were boosted by recurrent blood-stage infections following treatment. Of interest, in most recurrent P. vivax infections (4 out of 6 patients) DEKnull-2 IgG antibodies were sustained for 6 to 12 months. Polymorphisms in the ebp2 gene does not seem to explain EBP2 low immunogenicity as the ebp2 allele associated with the P.vivax outbreak presented high identity to the original EBP2 isolate used as recombinant protein. Although EBP2 antibodies were barely detectable after a primary episode of P.vivax infection, EBP2 was highly recognized by serum IgG from long-term malaria-exposed Amazonians (range from 35 to 92% according to previous malaria episodes). Taken together, the results showed that individuals with a single and brief exposure to P.vivax infection develop very low anti-EBP2 antibodies, which tend to increase after long-term malaria exposure. Finally, the findings highlighted the potential of DEKnull-2 as a vaccine candidate, as in non-immune individuals anti-DEKnull-2 IgG antibodies were boosted even after a brief exposure to P.vivax blood ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 6 e0010493 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Bárbara A S Lima Gabriela M Fernandes Letícia M Torres Camilla V Pires Jéssica R S Alves Sâmick L Moreira-Nascimento Maria Fernanda A Nascimento Sofia L Afonso Helena L Costa Isabela P Cerávolo Tais N Sousa Irene S Soares Francis B Ntumngia John H Adams Luzia H Carvalho Flora S Kano Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Plasmodium vivax blood-stage invasion into reticulocyte is critical for parasite development. Thus, validation of novel parasite invasion ligands is essential for malaria vaccine development. Recently, we demonstrated that EBP2, a Duffy binding protein (DBP) paralog, is antigenically distinct from DBP and could not be functionally inhibited by anti-DBP antibodies. Here, we took advantage of a small outbreak of P.vivax malaria, located in a non-malarious area of Brazil, to investigate for the first time IgM/IgG antibodies against EBP2 and DEKnull-2 (an engineering DBPII vaccine) among individuals who had their first and brief exposure to P.vivax (16 cases and 22 non-cases). Our experimental approach included 4 cross sectional surveys at 3-month interval (12-month follow-up). The results demonstrated that while a brief initial P.vivax infection was not efficient to induce IgM/ IgG antibodies to either EBP2 or DEKnull-2, IgG antibodies against DEKnull-2 (but not EBP2) were boosted by recurrent blood-stage infections following treatment. Of interest, in most recurrent P. vivax infections (4 out of 6 patients) DEKnull-2 IgG antibodies were sustained for 6 to 12 months. Polymorphisms in the ebp2 gene does not seem to explain EBP2 low immunogenicity as the ebp2 allele associated with the P.vivax outbreak presented high identity to the original EBP2 isolate used as recombinant protein. Although EBP2 antibodies were barely detectable after a primary episode of P.vivax infection, EBP2 was highly recognized by serum IgG from long-term malaria-exposed Amazonians (range from 35 to 92% according to previous malaria episodes). Taken together, the results showed that individuals with a single and brief exposure to P.vivax infection develop very low anti-EBP2 antibodies, which tend to increase after long-term malaria exposure. Finally, the findings highlighted the potential of DEKnull-2 as a vaccine candidate, as in non-immune individuals anti-DEKnull-2 IgG antibodies were boosted even after a brief exposure to P.vivax blood ... |
format |
Article in Journal/Newspaper |
author |
Bárbara A S Lima Gabriela M Fernandes Letícia M Torres Camilla V Pires Jéssica R S Alves Sâmick L Moreira-Nascimento Maria Fernanda A Nascimento Sofia L Afonso Helena L Costa Isabela P Cerávolo Tais N Sousa Irene S Soares Francis B Ntumngia John H Adams Luzia H Carvalho Flora S Kano |
author_facet |
Bárbara A S Lima Gabriela M Fernandes Letícia M Torres Camilla V Pires Jéssica R S Alves Sâmick L Moreira-Nascimento Maria Fernanda A Nascimento Sofia L Afonso Helena L Costa Isabela P Cerávolo Tais N Sousa Irene S Soares Francis B Ntumngia John H Adams Luzia H Carvalho Flora S Kano |
author_sort |
Bárbara A S Lima |
title |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. |
title_short |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. |
title_full |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. |
title_fullStr |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. |
title_full_unstemmed |
Antibody response to a new member of the DBL family (EBP2) after a brief Plasmodium vivax exposure. |
title_sort |
antibody response to a new member of the dbl family (ebp2) after a brief plasmodium vivax exposure. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010493 https://doaj.org/article/093e8018a76640ec9b67fa9b7e179577 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010493 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010493 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010493 https://doaj.org/article/093e8018a76640ec9b67fa9b7e179577 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010493 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
6 |
container_start_page |
e0010493 |
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1766347363196076032 |