Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis , cortisol implant, and their combination
Abstract Background The salmon louse is an ectoparasitic copepod that causes major economic losses in the aquaculture industry of Atlantic salmon. This host displays a high level of susceptibility to lice which can be accounted for by several factors including stress. In addition, the parasite itsel...
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ftdoajarticles:oai:doaj.org/article:0936ecbeb6b847ad8a9dfdf62c9e741a 2023-05-15T15:30:42+02:00 Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis , cortisol implant, and their combination Krasnov Aleksei Skugor Stanko Todorcevic Marijana Glover Kevin A Nilsen Frank 2012-04-01T00:00:00Z https://doi.org/10.1186/1471-2164-13-130 https://doaj.org/article/0936ecbeb6b847ad8a9dfdf62c9e741a EN eng BMC http://www.biomedcentral.com/1471-2164/13/130 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-13-130 1471-2164 https://doaj.org/article/0936ecbeb6b847ad8a9dfdf62c9e741a BMC Genomics, Vol 13, Iss 1, p 130 (2012) Biotechnology TP248.13-248.65 Genetics QH426-470 article 2012 ftdoajarticles https://doi.org/10.1186/1471-2164-13-130 2022-12-31T03:14:44Z Abstract Background The salmon louse is an ectoparasitic copepod that causes major economic losses in the aquaculture industry of Atlantic salmon. This host displays a high level of susceptibility to lice which can be accounted for by several factors including stress. In addition, the parasite itself acts as a potent stressor of the host, and outcomes of infection can depend on biotic and abiotic factors that stimulate production of cortisol. Consequently, examination of responses to infection with this parasite, in addition to stress hormone regulation in Atlantic salmon, is vital for better understanding of the host pathogen interaction. Results Atlantic salmon post smolts were organised into four experimental groups: lice + cortisol, lice + placebo, no lice + cortisol, no lice + placebo. Infection levels were equal in both treatments upon termination of the experiment. Gene expression changes in skin were assessed with 21 k oligonucleotide microarray and qPCR at the chalimus stage 18 days post infection at 9°C. The transcriptomic effects of hormone treatment were significantly greater than lice-infection induced changes. Cortisol stimulated expression of genes involved in metabolism of steroids and amino acids, chaperones, responses to oxidative stress and eicosanoid metabolism and suppressed genes related to antigen presentation, B and T cells, antiviral and inflammatory responses. Cortisol and lice equally down-regulated a large panel of motor proteins that can be important for wound contraction. Cortisol also suppressed multiple genes involved in wound healing, parts of which were activated by the parasite. Down-regulation of collagens and other structural proteins was in parallel with the induction of proteinases that degrade extracellular matrix (MMP9 and MMP13). Cortisol reduced expression of genes encoding proteins involved in formation of various tissue structures, regulators of cell differentiation and growth factors. Conclusions These results suggest that cortisol-induced stress does not affect the ... Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles BMC Genomics 13 1 130 |
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Directory of Open Access Journals: DOAJ Articles |
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Biotechnology TP248.13-248.65 Genetics QH426-470 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 Krasnov Aleksei Skugor Stanko Todorcevic Marijana Glover Kevin A Nilsen Frank Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis , cortisol implant, and their combination |
topic_facet |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background The salmon louse is an ectoparasitic copepod that causes major economic losses in the aquaculture industry of Atlantic salmon. This host displays a high level of susceptibility to lice which can be accounted for by several factors including stress. In addition, the parasite itself acts as a potent stressor of the host, and outcomes of infection can depend on biotic and abiotic factors that stimulate production of cortisol. Consequently, examination of responses to infection with this parasite, in addition to stress hormone regulation in Atlantic salmon, is vital for better understanding of the host pathogen interaction. Results Atlantic salmon post smolts were organised into four experimental groups: lice + cortisol, lice + placebo, no lice + cortisol, no lice + placebo. Infection levels were equal in both treatments upon termination of the experiment. Gene expression changes in skin were assessed with 21 k oligonucleotide microarray and qPCR at the chalimus stage 18 days post infection at 9°C. The transcriptomic effects of hormone treatment were significantly greater than lice-infection induced changes. Cortisol stimulated expression of genes involved in metabolism of steroids and amino acids, chaperones, responses to oxidative stress and eicosanoid metabolism and suppressed genes related to antigen presentation, B and T cells, antiviral and inflammatory responses. Cortisol and lice equally down-regulated a large panel of motor proteins that can be important for wound contraction. Cortisol also suppressed multiple genes involved in wound healing, parts of which were activated by the parasite. Down-regulation of collagens and other structural proteins was in parallel with the induction of proteinases that degrade extracellular matrix (MMP9 and MMP13). Cortisol reduced expression of genes encoding proteins involved in formation of various tissue structures, regulators of cell differentiation and growth factors. Conclusions These results suggest that cortisol-induced stress does not affect the ... |
format |
Article in Journal/Newspaper |
author |
Krasnov Aleksei Skugor Stanko Todorcevic Marijana Glover Kevin A Nilsen Frank |
author_facet |
Krasnov Aleksei Skugor Stanko Todorcevic Marijana Glover Kevin A Nilsen Frank |
author_sort |
Krasnov Aleksei |
title |
Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis , cortisol implant, and their combination |
title_short |
Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis , cortisol implant, and their combination |
title_full |
Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis , cortisol implant, and their combination |
title_fullStr |
Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis , cortisol implant, and their combination |
title_full_unstemmed |
Gene expression in Atlantic salmon skin in response to infection with the parasitic copepod Lepeophtheirus salmonis , cortisol implant, and their combination |
title_sort |
gene expression in atlantic salmon skin in response to infection with the parasitic copepod lepeophtheirus salmonis , cortisol implant, and their combination |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1471-2164-13-130 https://doaj.org/article/0936ecbeb6b847ad8a9dfdf62c9e741a |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
BMC Genomics, Vol 13, Iss 1, p 130 (2012) |
op_relation |
http://www.biomedcentral.com/1471-2164/13/130 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-13-130 1471-2164 https://doaj.org/article/0936ecbeb6b847ad8a9dfdf62c9e741a |
op_doi |
https://doi.org/10.1186/1471-2164-13-130 |
container_title |
BMC Genomics |
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13 |
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1 |
container_start_page |
130 |
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1766361146730741760 |