The use of oligonucleotide probes for meningococcal serotype characterization
In the present study we examine the potential use of oligonucleotide probes to characterize Neisseria meningitidis serotypes without the use of monoclonal antibodies (MAbs). Antigenic diversity on PorB protein forms the bases of serotyping method. However, the current panel of MAbs underestimated, b...
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Universidade de São Paulo (USP)
1998
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ftdoajarticles:oai:doaj.org/article:08d1a0b37d354b8a86676ced1625ebe7 2024-09-09T19:26:09+00:00 The use of oligonucleotide probes for meningococcal serotype characterization SACCHI Claudio Tavares LEMOS Ana Paula Silva de Whitney Anne M. MELLES Carmo Elias A. SOLARI Claude André FRASCH Carl E. MAYER Leonard W. 1998-01-01T00:00:00Z https://doaj.org/article/08d1a0b37d354b8a86676ced1625ebe7 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651998000200009 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/08d1a0b37d354b8a86676ced1625ebe7 Revista do Instituto de Medicina Tropical de São Paulo, Vol 40, Iss 2, Pp 113-117 (1998) Neisseria meningitidis Serotyping/Class 3 protein porB gene Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1998 ftdoajarticles 2024-08-05T17:49:30Z In the present study we examine the potential use of oligonucleotide probes to characterize Neisseria meningitidis serotypes without the use of monoclonal antibodies (MAbs). Antigenic diversity on PorB protein forms the bases of serotyping method. However, the current panel of MAbs underestimated, by at least 50% the PorB variability, presumably because reagents for several PorB variable regions (VRs) are lacking, or because a number of VR variants are not recognized by serotype-defining MAbs12. We analyzed the use of oligonucleotide probes to characterize serotype 10 and serotype 19 of N. meningitidis. The porB gene sequence for the prototype strain of serotype 10 was determined, aligned with 7 other porB sequences from different serotypes, and analysis of individual VRs were performed. The results of DNA probes 21U (VR1-A) and 615U (VR3-B) used against 72 N. meningitidis strains confirm that VR1 type A and VR3 type B encode epitopes for serotype-defined MAbs 19 and 10, respectively. The use of probes for characterizing serotypes possible can type 100% of the PorB VR diversity. It is a simple and rapid method specially useful for analysis of large number of samples. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Neisseria meningitidis Serotyping/Class 3 protein porB gene Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Neisseria meningitidis Serotyping/Class 3 protein porB gene Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 SACCHI Claudio Tavares LEMOS Ana Paula Silva de Whitney Anne M. MELLES Carmo Elias A. SOLARI Claude André FRASCH Carl E. MAYER Leonard W. The use of oligonucleotide probes for meningococcal serotype characterization |
topic_facet |
Neisseria meningitidis Serotyping/Class 3 protein porB gene Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
In the present study we examine the potential use of oligonucleotide probes to characterize Neisseria meningitidis serotypes without the use of monoclonal antibodies (MAbs). Antigenic diversity on PorB protein forms the bases of serotyping method. However, the current panel of MAbs underestimated, by at least 50% the PorB variability, presumably because reagents for several PorB variable regions (VRs) are lacking, or because a number of VR variants are not recognized by serotype-defining MAbs12. We analyzed the use of oligonucleotide probes to characterize serotype 10 and serotype 19 of N. meningitidis. The porB gene sequence for the prototype strain of serotype 10 was determined, aligned with 7 other porB sequences from different serotypes, and analysis of individual VRs were performed. The results of DNA probes 21U (VR1-A) and 615U (VR3-B) used against 72 N. meningitidis strains confirm that VR1 type A and VR3 type B encode epitopes for serotype-defined MAbs 19 and 10, respectively. The use of probes for characterizing serotypes possible can type 100% of the PorB VR diversity. It is a simple and rapid method specially useful for analysis of large number of samples. |
format |
Article in Journal/Newspaper |
author |
SACCHI Claudio Tavares LEMOS Ana Paula Silva de Whitney Anne M. MELLES Carmo Elias A. SOLARI Claude André FRASCH Carl E. MAYER Leonard W. |
author_facet |
SACCHI Claudio Tavares LEMOS Ana Paula Silva de Whitney Anne M. MELLES Carmo Elias A. SOLARI Claude André FRASCH Carl E. MAYER Leonard W. |
author_sort |
SACCHI Claudio Tavares |
title |
The use of oligonucleotide probes for meningococcal serotype characterization |
title_short |
The use of oligonucleotide probes for meningococcal serotype characterization |
title_full |
The use of oligonucleotide probes for meningococcal serotype characterization |
title_fullStr |
The use of oligonucleotide probes for meningococcal serotype characterization |
title_full_unstemmed |
The use of oligonucleotide probes for meningococcal serotype characterization |
title_sort |
use of oligonucleotide probes for meningococcal serotype characterization |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1998 |
url |
https://doaj.org/article/08d1a0b37d354b8a86676ced1625ebe7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 40, Iss 2, Pp 113-117 (1998) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651998000200009 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/08d1a0b37d354b8a86676ced1625ebe7 |
_version_ |
1809895813890965504 |