Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids
Pseudomonas spp. are widely distributed in various environments around the world. They are also common in the Antarctic regions. To date, almost 200 plasmids of Pseudomonas spp. have been sequenced, but only 12 of them were isolated from psychrotolerant strains. In this study, 15 novel plasmids of c...
Published in: | Genes |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2019
|
Subjects: | |
Online Access: | https://doi.org/10.3390/genes10110850 https://doaj.org/article/08bf6cee2cb44b5d8b1d63c897c1bf54 |
id |
ftdoajarticles:oai:doaj.org/article:08bf6cee2cb44b5d8b1d63c897c1bf54 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:08bf6cee2cb44b5d8b1d63c897c1bf54 2023-05-15T14:04:32+02:00 Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids Krzysztof Romaniuk Michal Styczynski Przemyslaw Decewicz Oliwia Buraczewska Witold Uhrynowski Marco Fondi Marcin Wolosiewicz Magdalena Szuplewska Lukasz Dziewit 2019-10-01T00:00:00Z https://doi.org/10.3390/genes10110850 https://doaj.org/article/08bf6cee2cb44b5d8b1d63c897c1bf54 EN eng MDPI AG https://www.mdpi.com/2073-4425/10/11/850 https://doaj.org/toc/2073-4425 2073-4425 doi:10.3390/genes10110850 https://doaj.org/article/08bf6cee2cb44b5d8b1d63c897c1bf54 Genes, Vol 10, Iss 11, p 850 (2019) antarctica biofilm horizontal gene transfer plasmid pseudomonas transposable element Genetics QH426-470 article 2019 ftdoajarticles https://doi.org/10.3390/genes10110850 2022-12-31T15:45:31Z Pseudomonas spp. are widely distributed in various environments around the world. They are also common in the Antarctic regions. To date, almost 200 plasmids of Pseudomonas spp. have been sequenced, but only 12 of them were isolated from psychrotolerant strains. In this study, 15 novel plasmids of cold-active Pseudomonas spp. originating from the King George Island (Antarctica) were characterized using a combined, structural and functional approach, including thorough genomic analyses, functional analyses of selected genetic modules, and identification of active transposable elements localized within the plasmids and comparative genomics. The analyses performed in this study increased the understanding of the horizontal transfer of plasmids found within Pseudomonas populations inhabiting Antarctic soils. It was shown that the majority of the studied plasmids are narrow-host-range replicons, whose transfer across taxonomic boundaries may be limited. Moreover, structural and functional analyses enabled identification and characterization of various accessory genetic modules, including genes encoding major pilin protein (PilA), that enhance biofilm formation, as well as active transposable elements. Furthermore, comparative genomic analyses revealed that the studied plasmids of Antarctic Pseudomonas spp. are unique, as they are highly dissimilar to the other known plasmids of Pseudomonas spp. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic King George Island Genes 10 11 850 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antarctica biofilm horizontal gene transfer plasmid pseudomonas transposable element Genetics QH426-470 |
spellingShingle |
antarctica biofilm horizontal gene transfer plasmid pseudomonas transposable element Genetics QH426-470 Krzysztof Romaniuk Michal Styczynski Przemyslaw Decewicz Oliwia Buraczewska Witold Uhrynowski Marco Fondi Marcin Wolosiewicz Magdalena Szuplewska Lukasz Dziewit Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids |
topic_facet |
antarctica biofilm horizontal gene transfer plasmid pseudomonas transposable element Genetics QH426-470 |
description |
Pseudomonas spp. are widely distributed in various environments around the world. They are also common in the Antarctic regions. To date, almost 200 plasmids of Pseudomonas spp. have been sequenced, but only 12 of them were isolated from psychrotolerant strains. In this study, 15 novel plasmids of cold-active Pseudomonas spp. originating from the King George Island (Antarctica) were characterized using a combined, structural and functional approach, including thorough genomic analyses, functional analyses of selected genetic modules, and identification of active transposable elements localized within the plasmids and comparative genomics. The analyses performed in this study increased the understanding of the horizontal transfer of plasmids found within Pseudomonas populations inhabiting Antarctic soils. It was shown that the majority of the studied plasmids are narrow-host-range replicons, whose transfer across taxonomic boundaries may be limited. Moreover, structural and functional analyses enabled identification and characterization of various accessory genetic modules, including genes encoding major pilin protein (PilA), that enhance biofilm formation, as well as active transposable elements. Furthermore, comparative genomic analyses revealed that the studied plasmids of Antarctic Pseudomonas spp. are unique, as they are highly dissimilar to the other known plasmids of Pseudomonas spp. |
format |
Article in Journal/Newspaper |
author |
Krzysztof Romaniuk Michal Styczynski Przemyslaw Decewicz Oliwia Buraczewska Witold Uhrynowski Marco Fondi Marcin Wolosiewicz Magdalena Szuplewska Lukasz Dziewit |
author_facet |
Krzysztof Romaniuk Michal Styczynski Przemyslaw Decewicz Oliwia Buraczewska Witold Uhrynowski Marco Fondi Marcin Wolosiewicz Magdalena Szuplewska Lukasz Dziewit |
author_sort |
Krzysztof Romaniuk |
title |
Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids |
title_short |
Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids |
title_full |
Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids |
title_fullStr |
Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids |
title_full_unstemmed |
Diversity and Horizontal Transfer of Antarctic Pseudomonas spp. Plasmids |
title_sort |
diversity and horizontal transfer of antarctic pseudomonas spp. plasmids |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://doi.org/10.3390/genes10110850 https://doaj.org/article/08bf6cee2cb44b5d8b1d63c897c1bf54 |
geographic |
Antarctic The Antarctic King George Island |
geographic_facet |
Antarctic The Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_source |
Genes, Vol 10, Iss 11, p 850 (2019) |
op_relation |
https://www.mdpi.com/2073-4425/10/11/850 https://doaj.org/toc/2073-4425 2073-4425 doi:10.3390/genes10110850 https://doaj.org/article/08bf6cee2cb44b5d8b1d63c897c1bf54 |
op_doi |
https://doi.org/10.3390/genes10110850 |
container_title |
Genes |
container_volume |
10 |
container_issue |
11 |
container_start_page |
850 |
_version_ |
1766275710610046976 |