PHENOTYPIC AND GENOTYPIC ANALYSIS OF AN ARCANOBACTERIUM PLURANIMALIUM ISOLATED FROM A MUSKOX (OVIBOS MOSCHATUS)

The present study was designed to characterize an Arcanobacterium pluranimalium strain isolated from a muskox ( Ovibos moschatus ) phenotypically, by MALDI-TOF MS analysis and genotypically using various molecular targets. The phenotypic properties, the MALDI-TOF MS analysis and sequencing the 16S r...

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Bibliographic Details
Main Authors: Siti Gusti Ningrum, Wyanda Arnafia, Osama Sammra, Mazen Alssahen, Jörn-Peter Wickhorst, Abdulwahed Ahmed Hassan, Christoph Lämmler, Ellen Prenger-Berninghoff, Markus Timke, Amir Abdulmawjood
Format: Article in Journal/Newspaper
Language:Bosnian
English
Croatian
srp
Published: Veterinary Faculty Sarajevo 2017
Subjects:
Veterinary medicine
SF600-1100
muskox
ovibos moschatus
Online Access:https://doaj.org/article/08b25e92e50f48539d33c43da4249410
Description
Summary:The present study was designed to characterize an Arcanobacterium pluranimalium strain isolated from a muskox ( Ovibos moschatus ) phenotypically, by MALDI-TOF MS analysis and genotypically using various molecular targets. The phenotypic properties, the MALDI-TOF MS analysis and sequencing the 16S rRNA gene, the β subunit of bacterial RNA polymerase encoding gene rpoB , the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap , the elongation factor tu encoding gene tuf and the pluranimaliumlysin encoding gene pla allowed a successful identification of the isolated strain as A. pluranimalium . Gene pla could also be detected by a previously described loop-mediated isothermal amplification (LAMP) assay. This is first report on the isolation and characterization of A. pluranimalium originated from a Muskox.

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