Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus
Objective: To isolate new endophytic fungus from Phaleria macrocarpa (P. macrocarpa) that is able to produce E2.2 compound. Methods: Endophytic fungi were isolated from P. macrocarpa. Morphological and molecular identification was done to determine the species of the endophytic fungus. High performa...
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Wolters Kluwer Medknow Publications
2016
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ftdoajarticles:oai:doaj.org/article:08822074af174f589de119f5fdd9ffa3 2023-05-15T15:06:40+02:00 Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus Beatrix Trikurnia Gasong Raymond Rubianto Tjandrawinata 2016-10-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.01.005 https://doaj.org/article/08822074af174f589de119f5fdd9ffa3 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115307462 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.01.005 https://doaj.org/article/08822074af174f589de119f5fdd9ffa3 Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 10, Pp 881-885 (2016) Endophytic fungi Phaleria macrocarpa Secondary metabolite Colletotrichum gloeosporioides E2.2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.01.005 2022-12-31T05:38:28Z Objective: To isolate new endophytic fungus from Phaleria macrocarpa (P. macrocarpa) that is able to produce E2.2 compound. Methods: Endophytic fungi were isolated from P. macrocarpa. Morphological and molecular identification was done to determine the species of the endophytic fungus. High performance liquid chromatography was used to determine the ability of this fungus to produce E2.2 compound and to quantify the total yield of E2.2 from fungal fermentation. Fermentation process was optimized by observing suitable medium, pH and length of fermentation process. Phloroglucinol and gallic acid addition were examined to determine the effect of each compound on E2.2 production. Results: One endophytic fungus was successfully isolated from P. macrocarpa plant. Morphological and molecular identification showed that it was a Colletotrichum gloeosporioides which belonged to Glomerellaceae family. This fungus showed highest production of E2.2 when incubated in potato dextrose broth with initial pH value of the medium at 5, and was incubated for 15 days. Phloroglucinol was found to better enhance E2.2 production. Conclusions: Colletotrichum gloeosporioides found in P. macrocarpa plant is promising as a potential alternative source of E2.2. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 10 881 885 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Endophytic fungi Phaleria macrocarpa Secondary metabolite Colletotrichum gloeosporioides E2.2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Endophytic fungi Phaleria macrocarpa Secondary metabolite Colletotrichum gloeosporioides E2.2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Beatrix Trikurnia Gasong Raymond Rubianto Tjandrawinata Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus |
topic_facet |
Endophytic fungi Phaleria macrocarpa Secondary metabolite Colletotrichum gloeosporioides E2.2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To isolate new endophytic fungus from Phaleria macrocarpa (P. macrocarpa) that is able to produce E2.2 compound. Methods: Endophytic fungi were isolated from P. macrocarpa. Morphological and molecular identification was done to determine the species of the endophytic fungus. High performance liquid chromatography was used to determine the ability of this fungus to produce E2.2 compound and to quantify the total yield of E2.2 from fungal fermentation. Fermentation process was optimized by observing suitable medium, pH and length of fermentation process. Phloroglucinol and gallic acid addition were examined to determine the effect of each compound on E2.2 production. Results: One endophytic fungus was successfully isolated from P. macrocarpa plant. Morphological and molecular identification showed that it was a Colletotrichum gloeosporioides which belonged to Glomerellaceae family. This fungus showed highest production of E2.2 when incubated in potato dextrose broth with initial pH value of the medium at 5, and was incubated for 15 days. Phloroglucinol was found to better enhance E2.2 production. Conclusions: Colletotrichum gloeosporioides found in P. macrocarpa plant is promising as a potential alternative source of E2.2. |
format |
Article in Journal/Newspaper |
author |
Beatrix Trikurnia Gasong Raymond Rubianto Tjandrawinata |
author_facet |
Beatrix Trikurnia Gasong Raymond Rubianto Tjandrawinata |
author_sort |
Beatrix Trikurnia Gasong |
title |
Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus |
title_short |
Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus |
title_full |
Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus |
title_fullStr |
Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus |
title_full_unstemmed |
Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus |
title_sort |
production of secondary metabolite e2.2 from phaleria macrocarpa endophytic fungus |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.01.005 https://doaj.org/article/08822074af174f589de119f5fdd9ffa3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 10, Pp 881-885 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115307462 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.01.005 https://doaj.org/article/08822074af174f589de119f5fdd9ffa3 |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.01.005 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
10 |
container_start_page |
881 |
op_container_end_page |
885 |
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1766338230243819520 |