Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil
ABSTRACT The genetic study of sandfly populations needs to be further explored given the importance of these insects for public health. Were sequenced the NDH4 mitochondrial gene from populations of Nyssomyia neivai from Doutor Camargo, Lobato, Japira, and Porto Rico, municipalities in the State of...
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ftdoajarticles:oai:doaj.org/article:086ba1ce16564f3f996c432f362a0aa6 2024-09-09T19:26:47+00:00 Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil Jaqueline de Carvalho Gasparotto Magda Clara Vieira da Costa-Ribeiro Vanete Thomaz-Soccol Sandra Mara Rodrigues da Silva Liebel Herintha Coeto Neitzke-Abreu Kárin Rosi Reinhold-Castro Edilson Colhera Cristovão Ueslei Teodoro https://doi.org/10.1590/s1678-9946201759010 https://doaj.org/article/086ba1ce16564f3f996c432f362a0aa6 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000206&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201759010 https://doaj.org/article/086ba1ce16564f3f996c432f362a0aa6 Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 Sandflies Lutzomyia mtDNA Phylogeny Genetic polymorphism Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/s1678-9946201759010 2024-08-05T17:49:30Z ABSTRACT The genetic study of sandfly populations needs to be further explored given the importance of these insects for public health. Were sequenced the NDH4 mitochondrial gene from populations of Nyssomyia neivai from Doutor Camargo, Lobato, Japira, and Porto Rico, municipalities in the State of Paraná, Brazil, to understand the genetic structure and gene flow. Eighty specimens of Ny. Neivai were sequenced, 20 from each municipality, and 269 base pairs were obtained. A total of 27 haplotypes and 28 polymorphic sites were found, along with a haplotypic diversity of 0.80696 and a nucleotide diversity of 0.00567. Haplotype H5, with 33 specimens, was the most common among the four populations. Only haplotypes H5 and H7 were present in all four populations. The population from Doutor Camargo showed the highest genetic diversity, and only this population shared haplotypes with those from the other municipalities. The highest number of haplotypes was sheared with Lobato which also had the highest number of unique haplotypes. This probably occurred because of constant anthropic changes that happened in the environment during the first half of the twentieth century, mainly after 1998. There was no significant correlation between genetic and geographical distances regarding these populations. However, the highest genetic and geographical distances, and the lowest gene flow were observed between Japira and Porto Rico. Geographical distance is a possible barrier between these municipalities through the blocking of haplotype sharing. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 59 0 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
Sandflies Lutzomyia mtDNA Phylogeny Genetic polymorphism Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Sandflies Lutzomyia mtDNA Phylogeny Genetic polymorphism Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Jaqueline de Carvalho Gasparotto Magda Clara Vieira da Costa-Ribeiro Vanete Thomaz-Soccol Sandra Mara Rodrigues da Silva Liebel Herintha Coeto Neitzke-Abreu Kárin Rosi Reinhold-Castro Edilson Colhera Cristovão Ueslei Teodoro Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil |
topic_facet |
Sandflies Lutzomyia mtDNA Phylogeny Genetic polymorphism Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT The genetic study of sandfly populations needs to be further explored given the importance of these insects for public health. Were sequenced the NDH4 mitochondrial gene from populations of Nyssomyia neivai from Doutor Camargo, Lobato, Japira, and Porto Rico, municipalities in the State of Paraná, Brazil, to understand the genetic structure and gene flow. Eighty specimens of Ny. Neivai were sequenced, 20 from each municipality, and 269 base pairs were obtained. A total of 27 haplotypes and 28 polymorphic sites were found, along with a haplotypic diversity of 0.80696 and a nucleotide diversity of 0.00567. Haplotype H5, with 33 specimens, was the most common among the four populations. Only haplotypes H5 and H7 were present in all four populations. The population from Doutor Camargo showed the highest genetic diversity, and only this population shared haplotypes with those from the other municipalities. The highest number of haplotypes was sheared with Lobato which also had the highest number of unique haplotypes. This probably occurred because of constant anthropic changes that happened in the environment during the first half of the twentieth century, mainly after 1998. There was no significant correlation between genetic and geographical distances regarding these populations. However, the highest genetic and geographical distances, and the lowest gene flow were observed between Japira and Porto Rico. Geographical distance is a possible barrier between these municipalities through the blocking of haplotype sharing. |
format |
Article in Journal/Newspaper |
author |
Jaqueline de Carvalho Gasparotto Magda Clara Vieira da Costa-Ribeiro Vanete Thomaz-Soccol Sandra Mara Rodrigues da Silva Liebel Herintha Coeto Neitzke-Abreu Kárin Rosi Reinhold-Castro Edilson Colhera Cristovão Ueslei Teodoro |
author_facet |
Jaqueline de Carvalho Gasparotto Magda Clara Vieira da Costa-Ribeiro Vanete Thomaz-Soccol Sandra Mara Rodrigues da Silva Liebel Herintha Coeto Neitzke-Abreu Kárin Rosi Reinhold-Castro Edilson Colhera Cristovão Ueslei Teodoro |
author_sort |
Jaqueline de Carvalho Gasparotto |
title |
Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil |
title_short |
Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil |
title_full |
Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil |
title_fullStr |
Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil |
title_full_unstemmed |
Genetic variability of populations of Nyssomyia neivai in the Northern State of Paraná, Brazil |
title_sort |
genetic variability of populations of nyssomyia neivai in the northern state of paraná, brazil |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/s1678-9946201759010 https://doaj.org/article/086ba1ce16564f3f996c432f362a0aa6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652017005000206&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946201759010 https://doaj.org/article/086ba1ce16564f3f996c432f362a0aa6 |
op_doi |
https://doi.org/10.1590/s1678-9946201759010 |
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Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
59 |
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