Peptides of marine animals as a potential source of natural antioxidants
Peptides extracted from tissues of 11 shellfish species ( Corbicula japonica, Mercenaria stimpsoni, Anadara broughtoni, Patinopecten yessoesis, Crenomytilus grayanus, Spisula sachalinensis, Maktra chinensis, Serripes groenlandicus, Glycymeris yessoensis, Callista brevisiphonata, Dosinia japonica ) a...
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Transactions of the Pacific Research Institute of Fisheries and Oceanography
2017
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Online Access: | https://doi.org/10.26428/1606-9919-2017-189-192-203 https://doaj.org/article/086ab2a2986a41188dc9da7a91215cfd |
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ftdoajarticles:oai:doaj.org/article:086ab2a2986a41188dc9da7a91215cfd 2023-10-09T21:55:54+02:00 Peptides of marine animals as a potential source of natural antioxidants Ekaterina P. Karaulova Anna I. Chepkasova 2017-06-01T00:00:00Z https://doi.org/10.26428/1606-9919-2017-189-192-203 https://doaj.org/article/086ab2a2986a41188dc9da7a91215cfd RU rus Transactions of the Pacific Research Institute of Fisheries and Oceanography https://izvestiya.tinro-center.ru/jour/article/view/195 https://doaj.org/toc/1606-9919 https://doaj.org/toc/2658-5510 1606-9919 2658-5510 doi:10.26428/1606-9919-2017-189-192-203 https://doaj.org/article/086ab2a2986a41188dc9da7a91215cfd Известия ТИНРО, Vol 189, Iss 2, Pp 192-203 (2017) двустворчатые моллюски гидролизат антиоксиданты антиоксидантная активность низкомолекулярные пептиды Aquaculture. Fisheries. Angling SH1-691 article 2017 ftdoajarticles https://doi.org/10.26428/1606-9919-2017-189-192-203 2023-09-10T00:44:26Z Peptides extracted from tissues of 11 shellfish species ( Corbicula japonica, Mercenaria stimpsoni, Anadara broughtoni, Patinopecten yessoesis, Crenomytilus grayanus, Spisula sachalinensis, Maktra chinensis, Serripes groenlandicus, Glycymeris yessoensis, Callista brevisiphonata, Dosinia japonica ) are tested for their antioxidant properties in different in vitro conditions. Frozen muscles and viscera of the shellfish were minced and subjected to water extraction of proteins and to hydrolysis with Protamex 1.5 MG. The peptides antioxidant activity was evaluated by assessing of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity on free radicals generated in oxidative systems. In the experiment, the high perfomance liquid chromatography (HPLC) system (Agilent Technologies 1260) included the frame TSKgel G 3000PWXL, the flow rate was 0.1 mL/min (0.1 N NaCI-20 mM Tris-HCI, pH 8.0), the detection was made under 280 nm. Molecular weight of the proteins was determined by comparison of their retention time with pure protein standards. The enzyme activity was high in conditions of pH in the range of 5.5-8.0, with the maximum under pH 7.0. Among 11 shellfish species, the highest antioxidant activity in water extract was observed for C. japonica , M. stimpsoni , C. brevisiphonata , G. yessoensis , and C. grayanus : 86.3, 68.7, 72.3, 90.2, and 67.5 mg of ascorbic acid/g, respectively. The DPPH radical scavenging activity increased with increasing of the hydrolysis degree. The 1-step hydrolysis with Protamex enhanced the DPPH scavenging activity for all samples, with the highest value for the protein hydrolysate of M. stimpsoni tissues (215 mg of ascorbic acid/g). Size of generated peptides is important for the antioxidant activity therefore molecular weight distribution of the peptides during the proteolysis was investigated using HPLC. Portion of the low molecular weight peptides (≤ 1 kDa) in the protein hydrolysate was increased in 4-14 % as compared with the starting protein. The antioxidant activity correlated ... Article in Journal/Newspaper Serripes groenlandicus Directory of Open Access Journals: DOAJ Articles Izvestiya TINRO 189 2 192 203 |
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Directory of Open Access Journals: DOAJ Articles |
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Russian |
topic |
двустворчатые моллюски гидролизат антиоксиданты антиоксидантная активность низкомолекулярные пептиды Aquaculture. Fisheries. Angling SH1-691 |
spellingShingle |
двустворчатые моллюски гидролизат антиоксиданты антиоксидантная активность низкомолекулярные пептиды Aquaculture. Fisheries. Angling SH1-691 Ekaterina P. Karaulova Anna I. Chepkasova Peptides of marine animals as a potential source of natural antioxidants |
topic_facet |
двустворчатые моллюски гидролизат антиоксиданты антиоксидантная активность низкомолекулярные пептиды Aquaculture. Fisheries. Angling SH1-691 |
description |
Peptides extracted from tissues of 11 shellfish species ( Corbicula japonica, Mercenaria stimpsoni, Anadara broughtoni, Patinopecten yessoesis, Crenomytilus grayanus, Spisula sachalinensis, Maktra chinensis, Serripes groenlandicus, Glycymeris yessoensis, Callista brevisiphonata, Dosinia japonica ) are tested for their antioxidant properties in different in vitro conditions. Frozen muscles and viscera of the shellfish were minced and subjected to water extraction of proteins and to hydrolysis with Protamex 1.5 MG. The peptides antioxidant activity was evaluated by assessing of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity on free radicals generated in oxidative systems. In the experiment, the high perfomance liquid chromatography (HPLC) system (Agilent Technologies 1260) included the frame TSKgel G 3000PWXL, the flow rate was 0.1 mL/min (0.1 N NaCI-20 mM Tris-HCI, pH 8.0), the detection was made under 280 nm. Molecular weight of the proteins was determined by comparison of their retention time with pure protein standards. The enzyme activity was high in conditions of pH in the range of 5.5-8.0, with the maximum under pH 7.0. Among 11 shellfish species, the highest antioxidant activity in water extract was observed for C. japonica , M. stimpsoni , C. brevisiphonata , G. yessoensis , and C. grayanus : 86.3, 68.7, 72.3, 90.2, and 67.5 mg of ascorbic acid/g, respectively. The DPPH radical scavenging activity increased with increasing of the hydrolysis degree. The 1-step hydrolysis with Protamex enhanced the DPPH scavenging activity for all samples, with the highest value for the protein hydrolysate of M. stimpsoni tissues (215 mg of ascorbic acid/g). Size of generated peptides is important for the antioxidant activity therefore molecular weight distribution of the peptides during the proteolysis was investigated using HPLC. Portion of the low molecular weight peptides (≤ 1 kDa) in the protein hydrolysate was increased in 4-14 % as compared with the starting protein. The antioxidant activity correlated ... |
format |
Article in Journal/Newspaper |
author |
Ekaterina P. Karaulova Anna I. Chepkasova |
author_facet |
Ekaterina P. Karaulova Anna I. Chepkasova |
author_sort |
Ekaterina P. Karaulova |
title |
Peptides of marine animals as a potential source of natural antioxidants |
title_short |
Peptides of marine animals as a potential source of natural antioxidants |
title_full |
Peptides of marine animals as a potential source of natural antioxidants |
title_fullStr |
Peptides of marine animals as a potential source of natural antioxidants |
title_full_unstemmed |
Peptides of marine animals as a potential source of natural antioxidants |
title_sort |
peptides of marine animals as a potential source of natural antioxidants |
publisher |
Transactions of the Pacific Research Institute of Fisheries and Oceanography |
publishDate |
2017 |
url |
https://doi.org/10.26428/1606-9919-2017-189-192-203 https://doaj.org/article/086ab2a2986a41188dc9da7a91215cfd |
genre |
Serripes groenlandicus |
genre_facet |
Serripes groenlandicus |
op_source |
Известия ТИНРО, Vol 189, Iss 2, Pp 192-203 (2017) |
op_relation |
https://izvestiya.tinro-center.ru/jour/article/view/195 https://doaj.org/toc/1606-9919 https://doaj.org/toc/2658-5510 1606-9919 2658-5510 doi:10.26428/1606-9919-2017-189-192-203 https://doaj.org/article/086ab2a2986a41188dc9da7a91215cfd |
op_doi |
https://doi.org/10.26428/1606-9919-2017-189-192-203 |
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Izvestiya TINRO |
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189 |
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2 |
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192 |
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203 |
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1779320123822178304 |