Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits
Background Quorum Sensing (QS) is a cell-to-cell communication system that bacteria utilize to adapt to the external environment by synthesizing and responding to signalling molecules called autoinducers. The psychrotrophic bacterium Aliivibrio wodanis 06/09/139, originally isolated from a winter ul...
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ftdoajarticles:oai:doaj.org/article:0846971e8aeb4e55aa9ce2bb6f4ebbc2 2023-10-01T03:54:46+02:00 Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits Amudha Deepalakshmi Maharajan Hilde Hansen Miriam Khider Nils Peder Willassen 2021-08-01T00:00:00Z https://doi.org/10.7717/peerj.11980 https://doaj.org/article/0846971e8aeb4e55aa9ce2bb6f4ebbc2 EN eng PeerJ Inc. https://peerj.com/articles/11980.pdf https://peerj.com/articles/11980/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.11980 2167-8359 https://doaj.org/article/0846971e8aeb4e55aa9ce2bb6f4ebbc2 PeerJ, Vol 9, p e11980 (2021) Quorum sensing AHL Aliivibrio wodanis Winter ulcer Atlantic salmon Motility Medicine R article 2021 ftdoajarticles https://doi.org/10.7717/peerj.11980 2023-09-03T00:42:42Z Background Quorum Sensing (QS) is a cell-to-cell communication system that bacteria utilize to adapt to the external environment by synthesizing and responding to signalling molecules called autoinducers. The psychrotrophic bacterium Aliivibrio wodanis 06/09/139, originally isolated from a winter ulcer of a reared Atlantic salmon, produces the autoinducer N-3-hydroxy-decanoyl-homoserine-lactone (3OHC10-HSL) and encodes the QS systems AinS/R and LuxS/PQ, and the master regulator LitR. However, the role of QS in this bacterium has not been investigated yet. Results In the present work we show that 3OHC10-HSL production is cell density and temperature-dependent in A. wodanis 06/09/139 with the highest production occurring at a low temperature (6 °C). Gene inactivation demonstrates that AinS is responsible for 3OHC10-HSL production and positively regulated by LitR. Inactivation of ainS and litR further show that QS is involved in the regulation of growth, motility, hemolysis, protease activity and siderophore production. Of these QS regulated activities, only the protease activity was found to be independent of LitR. Lastly, supernatants harvested from the wild type and the ΔainS and ΔlitR mutants at high cell densities show that inactivation of QS leads to a decreased cytopathogenic effect (CPE) in a cell culture assay, and strongest attenuation of the CPE was observed with supernatants harvested from the ΔlitR mutant. Conclusion A. wodanis 06/09/139 use QS to regulate a number of activities that may prove important for host colonization or interactions. The temperature of 6 °C that is in the temperature range at which winter ulcer occurs, plays a role in AHL production and development of CPE on a Chinook Salmon Embryo (CHSE) cell line. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles PeerJ 9 e11980 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Quorum sensing AHL Aliivibrio wodanis Winter ulcer Atlantic salmon Motility Medicine R |
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Quorum sensing AHL Aliivibrio wodanis Winter ulcer Atlantic salmon Motility Medicine R Amudha Deepalakshmi Maharajan Hilde Hansen Miriam Khider Nils Peder Willassen Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
topic_facet |
Quorum sensing AHL Aliivibrio wodanis Winter ulcer Atlantic salmon Motility Medicine R |
description |
Background Quorum Sensing (QS) is a cell-to-cell communication system that bacteria utilize to adapt to the external environment by synthesizing and responding to signalling molecules called autoinducers. The psychrotrophic bacterium Aliivibrio wodanis 06/09/139, originally isolated from a winter ulcer of a reared Atlantic salmon, produces the autoinducer N-3-hydroxy-decanoyl-homoserine-lactone (3OHC10-HSL) and encodes the QS systems AinS/R and LuxS/PQ, and the master regulator LitR. However, the role of QS in this bacterium has not been investigated yet. Results In the present work we show that 3OHC10-HSL production is cell density and temperature-dependent in A. wodanis 06/09/139 with the highest production occurring at a low temperature (6 °C). Gene inactivation demonstrates that AinS is responsible for 3OHC10-HSL production and positively regulated by LitR. Inactivation of ainS and litR further show that QS is involved in the regulation of growth, motility, hemolysis, protease activity and siderophore production. Of these QS regulated activities, only the protease activity was found to be independent of LitR. Lastly, supernatants harvested from the wild type and the ΔainS and ΔlitR mutants at high cell densities show that inactivation of QS leads to a decreased cytopathogenic effect (CPE) in a cell culture assay, and strongest attenuation of the CPE was observed with supernatants harvested from the ΔlitR mutant. Conclusion A. wodanis 06/09/139 use QS to regulate a number of activities that may prove important for host colonization or interactions. The temperature of 6 °C that is in the temperature range at which winter ulcer occurs, plays a role in AHL production and development of CPE on a Chinook Salmon Embryo (CHSE) cell line. |
format |
Article in Journal/Newspaper |
author |
Amudha Deepalakshmi Maharajan Hilde Hansen Miriam Khider Nils Peder Willassen |
author_facet |
Amudha Deepalakshmi Maharajan Hilde Hansen Miriam Khider Nils Peder Willassen |
author_sort |
Amudha Deepalakshmi Maharajan |
title |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_short |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_full |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_fullStr |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_full_unstemmed |
Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
title_sort |
quorum sensing in aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits |
publisher |
PeerJ Inc. |
publishDate |
2021 |
url |
https://doi.org/10.7717/peerj.11980 https://doaj.org/article/0846971e8aeb4e55aa9ce2bb6f4ebbc2 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PeerJ, Vol 9, p e11980 (2021) |
op_relation |
https://peerj.com/articles/11980.pdf https://peerj.com/articles/11980/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.11980 2167-8359 https://doaj.org/article/0846971e8aeb4e55aa9ce2bb6f4ebbc2 |
op_doi |
https://doi.org/10.7717/peerj.11980 |
container_title |
PeerJ |
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9 |
container_start_page |
e11980 |
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1778522675243646976 |