Leptospira spp. infection in sheep herds in southeast Brazil
Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The mic...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:0837078f32f34bbb977ddc18c89de833 2023-05-15T15:07:22+02:00 Leptospira spp. infection in sheep herds in southeast Brazil Priscila Barbante Fabio H Shimabukuro Helio Langoni Virgínia B Richini-Pereira Simone B Lucheis 2014-06-01T00:00:00Z https://doi.org/10.1186/1678-9199-20-20 https://doaj.org/article/0837078f32f34bbb977ddc18c89de833 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-20 https://doaj.org/article/0837078f32f34bbb977ddc18c89de833 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0, Pp 9-15 (2014) Leptospirosis Ovine Serology Culture PCR Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2014 ftdoajarticles https://doi.org/10.1186/1678-9199-20-20 2022-12-31T11:12:13Z Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp. Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative. Conclusions PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Slaughter ENVELOPE(-85.633,-85.633,-78.617,-78.617) Journal of Venomous Animals and Toxins including Tropical Diseases 20 1 20 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Leptospirosis Ovine Serology Culture PCR Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Leptospirosis Ovine Serology Culture PCR Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Priscila Barbante Fabio H Shimabukuro Helio Langoni Virgínia B Richini-Pereira Simone B Lucheis Leptospira spp. infection in sheep herds in southeast Brazil |
topic_facet |
Leptospirosis Ovine Serology Culture PCR Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Background With the aim of studying Leptospira spp. infection in sheep herds, blood samples and respective kidney and liver fragments were collected from 100 animals from twenty different properties during slaughter at a meat company in the Sorocaba region, São Paulo state, southeast Brazil. The microscopic agglutination test (MAT) was performed with 29 strains of Leptospira spp. To identify the agent in the liver and kidney, 100 samples of each tissue were submitted to culture in Fletcher medium and analyzed by the polymerase chain reaction (PCR) for Leptospira spp. Results MAT detected 23 samples serologically positive for one or more Leptospira spp. serovars and significantly more for Autumnalis. Eight (4%) samples were positive in culture (four kidneys and four livers), corresponding to five animals with positive serology (one animal simultaneously positive for both kidney and liver) and two negatives. PCR detected Leptospira spp. in 14 samples (seven kidneys and seven livers) corresponding to 12 positive animals (two animals simultaneously positive for kidney and liver), of which ten were serologically positive and two negative. Conclusions PCR was faster, more practical and more sensitive than culture for detecting leptospires. The results reinforce the importance of sheep in the epidemiological context of leptospirosis. |
format |
Article in Journal/Newspaper |
author |
Priscila Barbante Fabio H Shimabukuro Helio Langoni Virgínia B Richini-Pereira Simone B Lucheis |
author_facet |
Priscila Barbante Fabio H Shimabukuro Helio Langoni Virgínia B Richini-Pereira Simone B Lucheis |
author_sort |
Priscila Barbante |
title |
Leptospira spp. infection in sheep herds in southeast Brazil |
title_short |
Leptospira spp. infection in sheep herds in southeast Brazil |
title_full |
Leptospira spp. infection in sheep herds in southeast Brazil |
title_fullStr |
Leptospira spp. infection in sheep herds in southeast Brazil |
title_full_unstemmed |
Leptospira spp. infection in sheep herds in southeast Brazil |
title_sort |
leptospira spp. infection in sheep herds in southeast brazil |
publisher |
SciELO |
publishDate |
2014 |
url |
https://doi.org/10.1186/1678-9199-20-20 https://doaj.org/article/0837078f32f34bbb977ddc18c89de833 |
long_lat |
ENVELOPE(-85.633,-85.633,-78.617,-78.617) |
geographic |
Arctic Slaughter |
geographic_facet |
Arctic Slaughter |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 20, Iss 0, Pp 9-15 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200325&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-20-20 https://doaj.org/article/0837078f32f34bbb977ddc18c89de833 |
op_doi |
https://doi.org/10.1186/1678-9199-20-20 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
20 |
container_issue |
1 |
container_start_page |
20 |
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1766338885972918272 |