Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua
CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid–base re...
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ftdoajarticles:oai:doaj.org/article:08282907d8364479b2494ab25a5b1806 2023-05-15T15:27:20+02:00 Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua Marian Yong-An Hu Katharina eMichael Cornelia M. Kreiss Meike eStumpp Sam eDupont Yung-Che eTseng Magnus eLucassen 2016-06-01T00:00:00Z https://doi.org/10.3389/fphys.2016.00198 https://doaj.org/article/08282907d8364479b2494ab25a5b1806 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fphys.2016.00198/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2016.00198 https://doaj.org/article/08282907d8364479b2494ab25a5b1806 Frontiers in Physiology, Vol 7 (2016) ocean acidification teleosts thermal compensation INTESTINAL ION TRANSPORT bicarbonate level Physiology QP1-981 article 2016 ftdoajarticles https://doi.org/10.3389/fphys.2016.00198 2022-12-31T16:26:51Z CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid–base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for four weeks to three CO2 levels (550, 1,200 and 2,200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO3- cotransporter (NBC1), pendrin-like Cl-/HCO3- exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA) and Cl- channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO3- secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO3- levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans. Article in Journal/Newspaper atlantic cod Gadus morhua Ocean acidification Directory of Open Access Journals: DOAJ Articles Frontiers in Physiology 7 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
ocean acidification teleosts thermal compensation INTESTINAL ION TRANSPORT bicarbonate level Physiology QP1-981 |
spellingShingle |
ocean acidification teleosts thermal compensation INTESTINAL ION TRANSPORT bicarbonate level Physiology QP1-981 Marian Yong-An Hu Katharina eMichael Cornelia M. Kreiss Meike eStumpp Sam eDupont Yung-Che eTseng Magnus eLucassen Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua |
topic_facet |
ocean acidification teleosts thermal compensation INTESTINAL ION TRANSPORT bicarbonate level Physiology QP1-981 |
description |
CO2-driven seawater acidification has been demonstrated to enhance intestinal bicarbonate secretion rates in teleosts, leading to an increased release of CaCO3 under simulated ocean acidification scenarios. In this study, we investigated if increasing CO2 levels stimulate the intestinal acid–base regulatory machinery of Atlantic cod (Gadus morhua) and whether temperatures at the upper limit of thermal tolerance stimulate or counteract ion regulatory capacities. Juvenile G. morhua were acclimated for four weeks to three CO2 levels (550, 1,200 and 2,200 μatm) covering present and near-future natural variability, at optimum (10°C) and summer maximum temperature (18°C), respectively. Immunohistochemical analyses revealed the subcellular localization of ion transporters, including Na+/K+-ATPase (NKA), Na+/H+-exchanger 3 (NHE3), Na+/HCO3- cotransporter (NBC1), pendrin-like Cl-/HCO3- exchanger (SLC26a6), V-type H+-ATPase subunit a (VHA) and Cl- channel 3 (CLC3) in epithelial cells of the anterior intestine. At 10°C, proteins and mRNA were generally up-regulated for most transporters in the intestinal epithelium after acclimation to higher CO2 levels. This supports recent findings demonstrating increased intestinal HCO3- secretion rates in response to CO2 induced seawater acidification. At 18°C, mRNA expression and protein concentrations of most ion transporters remained unchanged or were even decreased, suggesting thermal compensation. This response may be energetically favorable to retain blood HCO3- levels to stabilize pHe, but may negatively affect intestinal salt and water resorption of marine teleosts in future oceans. |
format |
Article in Journal/Newspaper |
author |
Marian Yong-An Hu Katharina eMichael Cornelia M. Kreiss Meike eStumpp Sam eDupont Yung-Che eTseng Magnus eLucassen |
author_facet |
Marian Yong-An Hu Katharina eMichael Cornelia M. Kreiss Meike eStumpp Sam eDupont Yung-Che eTseng Magnus eLucassen |
author_sort |
Marian Yong-An Hu |
title |
Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua |
title_short |
Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua |
title_full |
Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua |
title_fullStr |
Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua |
title_full_unstemmed |
Temperature modulates the effects of ocean acidification on intestinal ion transport in Atlantic cod, Gadus morhua |
title_sort |
temperature modulates the effects of ocean acidification on intestinal ion transport in atlantic cod, gadus morhua |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
https://doi.org/10.3389/fphys.2016.00198 https://doaj.org/article/08282907d8364479b2494ab25a5b1806 |
genre |
atlantic cod Gadus morhua Ocean acidification |
genre_facet |
atlantic cod Gadus morhua Ocean acidification |
op_source |
Frontiers in Physiology, Vol 7 (2016) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fphys.2016.00198/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2016.00198 https://doaj.org/article/08282907d8364479b2494ab25a5b1806 |
op_doi |
https://doi.org/10.3389/fphys.2016.00198 |
container_title |
Frontiers in Physiology |
container_volume |
7 |
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1766357783466213376 |