Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom.
Micrurus is one of the four snake genera of medical importance in Brazil. Coral snakes have a broad geographic distribution from the southern United States to Argentina. Micrurine envenomation is characterized by neurotoxic symptoms leading to dyspnea and death. Moreover, various local manifestation...
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ftdoajarticles:oai:doaj.org/article:081f8b52ed204046b40abafd7f92799b 2023-05-15T15:12:45+02:00 Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom. Luciana Lyra Casais-E-Silva Catarina Teixeira 2017-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005874 https://doaj.org/article/081f8b52ed204046b40abafd7f92799b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5716551?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005874 https://doaj.org/article/081f8b52ed204046b40abafd7f92799b PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0005874 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005874 2022-12-31T12:25:19Z Micrurus is one of the four snake genera of medical importance in Brazil. Coral snakes have a broad geographic distribution from the southern United States to Argentina. Micrurine envenomation is characterized by neurotoxic symptoms leading to dyspnea and death. Moreover, various local manifestations, including edema formation, have been described in patients bitten by different species of Micrurus. Thus, we investigated the ability of Micrurus lemniscatus venom (MLV) to induce local edema. We also explored mechanisms underlying this effect, focusing on participation of neuropeptides and mast cells.Intraplantar injection of MLV (1-10 μg/paw) in rats caused dose- and time-dependent edema with a peak between 15 min and 1 h after injection. MLV also induced degranulation of peritoneal mast cells (MCs). MC depletion by compound 48/80 markedly reduced MLV-induced edema. Pre-treatment (30 min) of rats with either promethazine a histamine H1 receptor antagonist or methysergide, a nonselective 5-HT receptor antagonist, reduced MLV-induced edema. However, neither thioperamide, a histamine H3/H4 receptor antagonist, nor co-injection of MLV with HOE-140, a BK2 receptor antagonist, altered the response. Depletion of neuropeptides by capsaicin or treatment of animals with NK1- and NK2-receptor antagonists (SR 140333 and SR 48968, respectively) markedly reduced MLV-induced edema.In conclusion, MLV induces paw edema in rats by mechanisms involving activation of mast cells and substance P-releasing sensory C-fibers. Tachykinins NKA and NKB, histamine, and serotonin are major mediators of the MLV-induced edematogenic response. Targeting mast cell- and sensory C-fiber-derived mediators should be considered as potential therapeutic approaches to interrupt development of local edema induced by Micrurus venoms. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Argentina PLOS Neglected Tropical Diseases 11 11 e0005874 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Luciana Lyra Casais-E-Silva Catarina Teixeira Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Micrurus is one of the four snake genera of medical importance in Brazil. Coral snakes have a broad geographic distribution from the southern United States to Argentina. Micrurine envenomation is characterized by neurotoxic symptoms leading to dyspnea and death. Moreover, various local manifestations, including edema formation, have been described in patients bitten by different species of Micrurus. Thus, we investigated the ability of Micrurus lemniscatus venom (MLV) to induce local edema. We also explored mechanisms underlying this effect, focusing on participation of neuropeptides and mast cells.Intraplantar injection of MLV (1-10 μg/paw) in rats caused dose- and time-dependent edema with a peak between 15 min and 1 h after injection. MLV also induced degranulation of peritoneal mast cells (MCs). MC depletion by compound 48/80 markedly reduced MLV-induced edema. Pre-treatment (30 min) of rats with either promethazine a histamine H1 receptor antagonist or methysergide, a nonselective 5-HT receptor antagonist, reduced MLV-induced edema. However, neither thioperamide, a histamine H3/H4 receptor antagonist, nor co-injection of MLV with HOE-140, a BK2 receptor antagonist, altered the response. Depletion of neuropeptides by capsaicin or treatment of animals with NK1- and NK2-receptor antagonists (SR 140333 and SR 48968, respectively) markedly reduced MLV-induced edema.In conclusion, MLV induces paw edema in rats by mechanisms involving activation of mast cells and substance P-releasing sensory C-fibers. Tachykinins NKA and NKB, histamine, and serotonin are major mediators of the MLV-induced edematogenic response. Targeting mast cell- and sensory C-fiber-derived mediators should be considered as potential therapeutic approaches to interrupt development of local edema induced by Micrurus venoms. |
format |
Article in Journal/Newspaper |
author |
Luciana Lyra Casais-E-Silva Catarina Teixeira |
author_facet |
Luciana Lyra Casais-E-Silva Catarina Teixeira |
author_sort |
Luciana Lyra Casais-E-Silva |
title |
Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom. |
title_short |
Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom. |
title_full |
Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom. |
title_fullStr |
Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom. |
title_full_unstemmed |
Neurogenic mediators contribute to local edema induced by Micrurus lemniscatus venom. |
title_sort |
neurogenic mediators contribute to local edema induced by micrurus lemniscatus venom. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005874 https://doaj.org/article/081f8b52ed204046b40abafd7f92799b |
geographic |
Arctic Argentina |
geographic_facet |
Arctic Argentina |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0005874 (2017) |
op_relation |
http://europepmc.org/articles/PMC5716551?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005874 https://doaj.org/article/081f8b52ed204046b40abafd7f92799b |
op_doi |
https://doi.org/10.1371/journal.pntd.0005874 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
11 |
container_start_page |
e0005874 |
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1766343389007052800 |