Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh.
There is growing interest in local elimination of soil-transmitted helminth (STH) infection in endemic settings. In such settings, highly sensitive diagnostics are needed to detect STH infection. We compared double-slide Kato-Katz, the most commonly used copromicroscopic detection method, to multi-p...
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2020
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Online Access: | https://doi.org/10.1371/journal.pntd.0008087 https://doaj.org/article/0811e2a38be5469eb3308a7b8f702506 |
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ftdoajarticles:oai:doaj.org/article:0811e2a38be5469eb3308a7b8f702506 2023-05-15T15:12:49+02:00 Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. Jade Benjamin-Chung Nils Pilotte Ayse Ercumen Jessica R Grant Jacqueline R M A Maasch Andrew M Gonzalez Ashanta C Ester Benjamin F Arnold Mahbubur Rahman Rashidul Haque Alan E Hubbard Stephen P Luby Steven A Williams John M Colford 2020-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008087 https://doaj.org/article/0811e2a38be5469eb3308a7b8f702506 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008087 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008087 https://doaj.org/article/0811e2a38be5469eb3308a7b8f702506 PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008087 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008087 2022-12-31T07:51:24Z There is growing interest in local elimination of soil-transmitted helminth (STH) infection in endemic settings. In such settings, highly sensitive diagnostics are needed to detect STH infection. We compared double-slide Kato-Katz, the most commonly used copromicroscopic detection method, to multi-parallel quantitative polymerase chain reaction (qPCR) in 2,799 stool samples from children aged 2-12 years in a setting in rural Bangladesh with predominantly low STH infection intensity. We estimated the sensitivity and specificity of each diagnostic using Bayesian latent class analysis. Compared to double-slide Kato-Katz, STH prevalence using qPCR was almost 3-fold higher for hookworm species and nearly 2-fold higher for Trichuris trichiura. Ascaris lumbricoides prevalence was lower using qPCR, and 26% of samples classified as A. lumbricoides positive by Kato-Katz were negative by qPCR. Amplicon sequencing of the 18S rDNA from 10 samples confirmed that A. lumbricoides was absent in samples classified as positive by Kato-Katz and negative by qPCR. The sensitivity of Kato-Katz was 49% for A. lumbricoides, 32% for hookworm, and 52% for T. trichiura; the sensitivity of qPCR was 79% for A. lumbricoides, 93% for hookworm, and 90% for T. trichiura. Specificity was ≥ 97% for both tests for all STH except for Kato-Katz for A. lumbricoides (specificity = 68%). There were moderate negative, monotonic correlations between qPCR cycle quantification values and eggs per gram quantified by Kato-Katz. While it is widely assumed that double-slide Kato-Katz has few false positives, our results indicate otherwise and highlight inherent limitations of the Kato-Katz technique. qPCR had higher sensitivity than Kato-Katz in this low intensity infection setting. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 4 e0008087 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jade Benjamin-Chung Nils Pilotte Ayse Ercumen Jessica R Grant Jacqueline R M A Maasch Andrew M Gonzalez Ashanta C Ester Benjamin F Arnold Mahbubur Rahman Rashidul Haque Alan E Hubbard Stephen P Luby Steven A Williams John M Colford Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
There is growing interest in local elimination of soil-transmitted helminth (STH) infection in endemic settings. In such settings, highly sensitive diagnostics are needed to detect STH infection. We compared double-slide Kato-Katz, the most commonly used copromicroscopic detection method, to multi-parallel quantitative polymerase chain reaction (qPCR) in 2,799 stool samples from children aged 2-12 years in a setting in rural Bangladesh with predominantly low STH infection intensity. We estimated the sensitivity and specificity of each diagnostic using Bayesian latent class analysis. Compared to double-slide Kato-Katz, STH prevalence using qPCR was almost 3-fold higher for hookworm species and nearly 2-fold higher for Trichuris trichiura. Ascaris lumbricoides prevalence was lower using qPCR, and 26% of samples classified as A. lumbricoides positive by Kato-Katz were negative by qPCR. Amplicon sequencing of the 18S rDNA from 10 samples confirmed that A. lumbricoides was absent in samples classified as positive by Kato-Katz and negative by qPCR. The sensitivity of Kato-Katz was 49% for A. lumbricoides, 32% for hookworm, and 52% for T. trichiura; the sensitivity of qPCR was 79% for A. lumbricoides, 93% for hookworm, and 90% for T. trichiura. Specificity was ≥ 97% for both tests for all STH except for Kato-Katz for A. lumbricoides (specificity = 68%). There were moderate negative, monotonic correlations between qPCR cycle quantification values and eggs per gram quantified by Kato-Katz. While it is widely assumed that double-slide Kato-Katz has few false positives, our results indicate otherwise and highlight inherent limitations of the Kato-Katz technique. qPCR had higher sensitivity than Kato-Katz in this low intensity infection setting. |
format |
Article in Journal/Newspaper |
author |
Jade Benjamin-Chung Nils Pilotte Ayse Ercumen Jessica R Grant Jacqueline R M A Maasch Andrew M Gonzalez Ashanta C Ester Benjamin F Arnold Mahbubur Rahman Rashidul Haque Alan E Hubbard Stephen P Luby Steven A Williams John M Colford |
author_facet |
Jade Benjamin-Chung Nils Pilotte Ayse Ercumen Jessica R Grant Jacqueline R M A Maasch Andrew M Gonzalez Ashanta C Ester Benjamin F Arnold Mahbubur Rahman Rashidul Haque Alan E Hubbard Stephen P Luby Steven A Williams John M Colford |
author_sort |
Jade Benjamin-Chung |
title |
Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. |
title_short |
Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. |
title_full |
Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. |
title_fullStr |
Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. |
title_full_unstemmed |
Comparison of multi-parallel qPCR and double-slide Kato-Katz for detection of soil-transmitted helminth infection among children in rural Bangladesh. |
title_sort |
comparison of multi-parallel qpcr and double-slide kato-katz for detection of soil-transmitted helminth infection among children in rural bangladesh. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008087 https://doaj.org/article/0811e2a38be5469eb3308a7b8f702506 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 4, p e0008087 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008087 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008087 https://doaj.org/article/0811e2a38be5469eb3308a7b8f702506 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008087 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
4 |
container_start_page |
e0008087 |
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1766343460336435200 |