Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti.
The yellow fever mosquito (Aedes aegypti), is the primary vector of dengue, Zika, and chikungunya fever, among other arboviral diseases. It is also a popular laboratory model in vector biology due to its ease of rearing and manipulation in the lab. Established laboratory strains have been used world...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0007930 https://doaj.org/article/080b13ca289b4a15b9411c5f3e2d0122 |
id |
ftdoajarticles:oai:doaj.org/article:080b13ca289b4a15b9411c5f3e2d0122 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:080b13ca289b4a15b9411c5f3e2d0122 2023-05-15T15:09:27+02:00 Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti. Andrea Gloria-Soria John Soghigian David Kellner Jeffrey R Powell 2019-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007930 https://doaj.org/article/080b13ca289b4a15b9411c5f3e2d0122 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007930 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007930 https://doaj.org/article/080b13ca289b4a15b9411c5f3e2d0122 PLoS Neglected Tropical Diseases, Vol 13, Iss 12, p e0007930 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007930 2022-12-31T11:44:55Z The yellow fever mosquito (Aedes aegypti), is the primary vector of dengue, Zika, and chikungunya fever, among other arboviral diseases. It is also a popular laboratory model in vector biology due to its ease of rearing and manipulation in the lab. Established laboratory strains have been used worldwide in thousands of studies for decades. Laboratory evolution of reference strains and contamination among strains are potential severe problems that could dramatically change experimental outcomes and thus is a concern in vector biology. We analyzed laboratory and field colonies of Ae. aegypti and an Ae. aegypti-derived cell line (Aag2) using 12 microsatellites and ~20,000 SNPs to determine the extent of divergence among laboratory strains and relationships to their wild relatives. We found that 1) laboratory populations are less genetically variable than their field counterparts; 2) colonies bearing the same name obtained from different laboratories may be highly divergent; 3) present genetic composition of the LVP strain used as the genome reference is incompatible with its presumed origin; 4) we document changes in two wild caught colonies over ~16 generations of colonization; and 5) the Aag2 Ae. aegypti cell line has experienced minimal genetic changes within and across laboratories. These results illustrate the degree of variability within and among strains of Ae. aegypti, with implications for cross-study comparisons, and highlight the need of a common mosquito repository and the implementation of strain validation tools. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 12 e0007930 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Andrea Gloria-Soria John Soghigian David Kellner Jeffrey R Powell Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The yellow fever mosquito (Aedes aegypti), is the primary vector of dengue, Zika, and chikungunya fever, among other arboviral diseases. It is also a popular laboratory model in vector biology due to its ease of rearing and manipulation in the lab. Established laboratory strains have been used worldwide in thousands of studies for decades. Laboratory evolution of reference strains and contamination among strains are potential severe problems that could dramatically change experimental outcomes and thus is a concern in vector biology. We analyzed laboratory and field colonies of Ae. aegypti and an Ae. aegypti-derived cell line (Aag2) using 12 microsatellites and ~20,000 SNPs to determine the extent of divergence among laboratory strains and relationships to their wild relatives. We found that 1) laboratory populations are less genetically variable than their field counterparts; 2) colonies bearing the same name obtained from different laboratories may be highly divergent; 3) present genetic composition of the LVP strain used as the genome reference is incompatible with its presumed origin; 4) we document changes in two wild caught colonies over ~16 generations of colonization; and 5) the Aag2 Ae. aegypti cell line has experienced minimal genetic changes within and across laboratories. These results illustrate the degree of variability within and among strains of Ae. aegypti, with implications for cross-study comparisons, and highlight the need of a common mosquito repository and the implementation of strain validation tools. |
format |
Article in Journal/Newspaper |
author |
Andrea Gloria-Soria John Soghigian David Kellner Jeffrey R Powell |
author_facet |
Andrea Gloria-Soria John Soghigian David Kellner Jeffrey R Powell |
author_sort |
Andrea Gloria-Soria |
title |
Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti. |
title_short |
Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti. |
title_full |
Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti. |
title_fullStr |
Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti. |
title_full_unstemmed |
Genetic diversity of laboratory strains and implications for research: The case of Aedes aegypti. |
title_sort |
genetic diversity of laboratory strains and implications for research: the case of aedes aegypti. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007930 https://doaj.org/article/080b13ca289b4a15b9411c5f3e2d0122 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 12, p e0007930 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007930 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007930 https://doaj.org/article/080b13ca289b4a15b9411c5f3e2d0122 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007930 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
12 |
container_start_page |
e0007930 |
_version_ |
1766340650212524032 |