Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin
The microphthalmia-associated transcription factor (MITF) is an important transcription factor that plays a key role in melanogenesis, cell proliferation, survival and immune defense in vertebrate. However, its function and function mechanism in bivalve are still rarely known. In this research, firs...
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ftdoajarticles:oai:doaj.org/article:075e1cd2845c46f58ad4a6fd3b87e1ed 2023-05-15T15:59:05+02:00 Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin Feifei Yu Yishan Lu Zhiming Zhong Bingliang Qu Meifang Wang Xiangyong Yu Jiayu Chen 2021-05-01T00:00:00Z https://doi.org/10.3389/fimmu.2021.626493 https://doaj.org/article/075e1cd2845c46f58ad4a6fd3b87e1ed EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fimmu.2021.626493/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2021.626493 https://doaj.org/article/075e1cd2845c46f58ad4a6fd3b87e1ed Frontiers in Immunology, Vol 12 (2021) Mitf melanin tyrosinase innate immunity Pteria penguin Immunologic diseases. Allergy RC581-607 article 2021 ftdoajarticles https://doi.org/10.3389/fimmu.2021.626493 2022-12-31T06:45:09Z The microphthalmia-associated transcription factor (MITF) is an important transcription factor that plays a key role in melanogenesis, cell proliferation, survival and immune defense in vertebrate. However, its function and function mechanism in bivalve are still rarely known. In this research, first, a Mitf gene was characterized from Pteria penguin (P. penguin). The PpMitf contained an open reading frame of 1,350 bp, encoding a peptide of 449 deduced amino acids with a highly conserved basic helix-loop-helix-leucine zipper (bHLH-LZ) domain. The PpMITF shared 55.7% identity with amino acid sequence of Crassostrea gigas (C. gigas). Tissue distribution analysis revealed that PpMitf was highly expressed in mantle and hemocytes, which were important tissues for color formation and innate immunity. Second, the functions of PpMitf in melanin synthesis and innate immunity were identified. The PpMitf silencing significantly decreased the tyrosinase activity and melanin content, indicating PpMitf involved in melanin synthesis of P. penguin. Meanwhile, the PpMitf silencing clearly down-regulated the expression of PpBcl2 (B cell lymphoma/leukemia-2 gene) and antibacterial activity of hemolymph supernatant, indicating that PpMitf involved in innate immunity of P. penguin. Third, the function mechanism of PpMitf in immunity was analyzed. The promoter sequence analysis of tyrosinase (Tyr) revealed two highly conserved E-box elements, which were specifically recognized by HLH-LZ of MITF. The luciferase activities analysis showed that Mitf could activate the E-box in Tyr promoter through highly conserved bHLH-LZ domain, and demonstrated that PpMitf involved in melanin synthesis and innate immunity by regulating tyrosinase expression. Finally, melanin from P. penguin, the final production of Mitf-Tyr-melanin pathway, was confirmed to have direct antibacterial activity. The results collectively demonstrated that PpMitf played a key role in innate immunity through activating tyrosinase-mediated melanin synthesis in P. penguin. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 12 |
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ftdoajarticles |
language |
English |
topic |
Mitf melanin tyrosinase innate immunity Pteria penguin Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Mitf melanin tyrosinase innate immunity Pteria penguin Immunologic diseases. Allergy RC581-607 Feifei Yu Yishan Lu Zhiming Zhong Bingliang Qu Meifang Wang Xiangyong Yu Jiayu Chen Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin |
topic_facet |
Mitf melanin tyrosinase innate immunity Pteria penguin Immunologic diseases. Allergy RC581-607 |
description |
The microphthalmia-associated transcription factor (MITF) is an important transcription factor that plays a key role in melanogenesis, cell proliferation, survival and immune defense in vertebrate. However, its function and function mechanism in bivalve are still rarely known. In this research, first, a Mitf gene was characterized from Pteria penguin (P. penguin). The PpMitf contained an open reading frame of 1,350 bp, encoding a peptide of 449 deduced amino acids with a highly conserved basic helix-loop-helix-leucine zipper (bHLH-LZ) domain. The PpMITF shared 55.7% identity with amino acid sequence of Crassostrea gigas (C. gigas). Tissue distribution analysis revealed that PpMitf was highly expressed in mantle and hemocytes, which were important tissues for color formation and innate immunity. Second, the functions of PpMitf in melanin synthesis and innate immunity were identified. The PpMitf silencing significantly decreased the tyrosinase activity and melanin content, indicating PpMitf involved in melanin synthesis of P. penguin. Meanwhile, the PpMitf silencing clearly down-regulated the expression of PpBcl2 (B cell lymphoma/leukemia-2 gene) and antibacterial activity of hemolymph supernatant, indicating that PpMitf involved in innate immunity of P. penguin. Third, the function mechanism of PpMitf in immunity was analyzed. The promoter sequence analysis of tyrosinase (Tyr) revealed two highly conserved E-box elements, which were specifically recognized by HLH-LZ of MITF. The luciferase activities analysis showed that Mitf could activate the E-box in Tyr promoter through highly conserved bHLH-LZ domain, and demonstrated that PpMitf involved in melanin synthesis and innate immunity by regulating tyrosinase expression. Finally, melanin from P. penguin, the final production of Mitf-Tyr-melanin pathway, was confirmed to have direct antibacterial activity. The results collectively demonstrated that PpMitf played a key role in innate immunity through activating tyrosinase-mediated melanin synthesis in P. penguin. |
format |
Article in Journal/Newspaper |
author |
Feifei Yu Yishan Lu Zhiming Zhong Bingliang Qu Meifang Wang Xiangyong Yu Jiayu Chen |
author_facet |
Feifei Yu Yishan Lu Zhiming Zhong Bingliang Qu Meifang Wang Xiangyong Yu Jiayu Chen |
author_sort |
Feifei Yu |
title |
Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin |
title_short |
Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin |
title_full |
Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin |
title_fullStr |
Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin |
title_full_unstemmed |
Mitf Involved in Innate Immunity by Activating Tyrosinase-Mediated Melanin Synthesis in Pteria penguin |
title_sort |
mitf involved in innate immunity by activating tyrosinase-mediated melanin synthesis in pteria penguin |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.626493 https://doaj.org/article/075e1cd2845c46f58ad4a6fd3b87e1ed |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Immunology, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.626493/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2021.626493 https://doaj.org/article/075e1cd2845c46f58ad4a6fd3b87e1ed |
op_doi |
https://doi.org/10.3389/fimmu.2021.626493 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
_version_ |
1766394874745061376 |