Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions.
Trypanosoma vivax is one of the most common parasites responsible for animal trypanosomosis, and although this disease is widespread in Africa and Latin America, very few studies have been conducted on the parasite's biology. This is in part due to the fact that no reproducible experimental met...
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ftdoajarticles:oai:doaj.org/article:074eb98a21c34660ab09e56aa35cbd4c 2023-05-15T15:09:19+02:00 Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions. Simon D'Archivio Mathieu Medina Alain Cosson Nathalie Chamond Brice Rotureau Paola Minoprio Sophie Goyard 2011-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001461 https://doaj.org/article/074eb98a21c34660ab09e56aa35cbd4c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3246432?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001461 https://doaj.org/article/074eb98a21c34660ab09e56aa35cbd4c PLoS Neglected Tropical Diseases, Vol 5, Iss 12, p e1461 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001461 2022-12-31T10:16:37Z Trypanosoma vivax is one of the most common parasites responsible for animal trypanosomosis, and although this disease is widespread in Africa and Latin America, very few studies have been conducted on the parasite's biology. This is in part due to the fact that no reproducible experimental methods had been developed to maintain the different evolutive forms of this trypanosome under laboratory conditions. Appropriate protocols were developed in the 1990s for the axenic maintenance of three major animal Trypanosoma species: T. b. brucei, T. congolense and T. vivax. These pioneer studies rapidly led to the successful genetic manipulation of T. b. brucei and T. congolense. Advances were made in the understanding of these parasites' biology and virulence, and new drug targets were identified. By contrast, challenging in vitro conditions have been developed for T. vivax in the past, and this per se has contributed to defer both its genetic manipulation and subsequent gene function studies. Here we report on the optimization of non-infective T. vivax epimastigote axenic cultures and on the process of parasite in vitro differentiation into metacyclic infective forms. We have also constructed the first T. vivax specific expression vector that drives constitutive expression of the luciferase reporter gene. This vector was then used to establish and optimize epimastigote transfection. We then developed highly reproducible conditions that can be used to obtain and select stably transfected mutants that continue metacyclogenesis and are infectious in immunocompetent rodents. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 12 e1461 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Simon D'Archivio Mathieu Medina Alain Cosson Nathalie Chamond Brice Rotureau Paola Minoprio Sophie Goyard Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trypanosoma vivax is one of the most common parasites responsible for animal trypanosomosis, and although this disease is widespread in Africa and Latin America, very few studies have been conducted on the parasite's biology. This is in part due to the fact that no reproducible experimental methods had been developed to maintain the different evolutive forms of this trypanosome under laboratory conditions. Appropriate protocols were developed in the 1990s for the axenic maintenance of three major animal Trypanosoma species: T. b. brucei, T. congolense and T. vivax. These pioneer studies rapidly led to the successful genetic manipulation of T. b. brucei and T. congolense. Advances were made in the understanding of these parasites' biology and virulence, and new drug targets were identified. By contrast, challenging in vitro conditions have been developed for T. vivax in the past, and this per se has contributed to defer both its genetic manipulation and subsequent gene function studies. Here we report on the optimization of non-infective T. vivax epimastigote axenic cultures and on the process of parasite in vitro differentiation into metacyclic infective forms. We have also constructed the first T. vivax specific expression vector that drives constitutive expression of the luciferase reporter gene. This vector was then used to establish and optimize epimastigote transfection. We then developed highly reproducible conditions that can be used to obtain and select stably transfected mutants that continue metacyclogenesis and are infectious in immunocompetent rodents. |
format |
Article in Journal/Newspaper |
author |
Simon D'Archivio Mathieu Medina Alain Cosson Nathalie Chamond Brice Rotureau Paola Minoprio Sophie Goyard |
author_facet |
Simon D'Archivio Mathieu Medina Alain Cosson Nathalie Chamond Brice Rotureau Paola Minoprio Sophie Goyard |
author_sort |
Simon D'Archivio |
title |
Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions. |
title_short |
Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions. |
title_full |
Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions. |
title_fullStr |
Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions. |
title_full_unstemmed |
Genetic engineering of Trypanosoma (Dutonella) vivax and in vitro differentiation under axenic conditions. |
title_sort |
genetic engineering of trypanosoma (dutonella) vivax and in vitro differentiation under axenic conditions. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001461 https://doaj.org/article/074eb98a21c34660ab09e56aa35cbd4c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 12, p e1461 (2011) |
op_relation |
http://europepmc.org/articles/PMC3246432?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001461 https://doaj.org/article/074eb98a21c34660ab09e56aa35cbd4c |
op_doi |
https://doi.org/10.1371/journal.pntd.0001461 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
12 |
container_start_page |
e1461 |
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