Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis
In human toxocariasis, there are few approaches using immunological markers for diagnosis and therapeutic assessment. An immunoblot (IB) assay using excretory-secretory Toxocara canis antigen was standardized for monitoring IgG, IgE and IgA antibodies in 27 children with toxocariasis (23 visceral, t...
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ftdoajarticles:oai:doaj.org/article:071a6fbf272b4be9b8a7a31ccc15057f 2024-09-09T19:26:26+00:00 Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis Guita Rubinsky-Elefant Sumie Hoshino-Shimizu Cristina Miuki Abe Jacob Maria Carmen Arroyo Sanchez Antonio Walter Ferreira 2011-04-01T00:00:00Z https://doi.org/10.1590/S0036-46652011000200001 https://doaj.org/article/071a6fbf272b4be9b8a7a31ccc15057f EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652011000200001&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652011000200001 https://doaj.org/article/071a6fbf272b4be9b8a7a31ccc15057f Revista do Instituto de Medicina Tropical de São Paulo, Vol 53, Iss 2, Pp 61-65 (2011) Toxocariasis Immunoblot assay Immunoglobulin isotypes Chemotherapy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1590/S0036-46652011000200001 2024-08-05T17:49:31Z In human toxocariasis, there are few approaches using immunological markers for diagnosis and therapeutic assessment. An immunoblot (IB) assay using excretory-secretory Toxocara canis antigen was standardized for monitoring IgG, IgE and IgA antibodies in 27 children with toxocariasis (23 visceral, three mixed visceral and ocular, and one ocular form) for 22-116 months after chemotherapy. IB sensitivity was 100% for IgG antibodies to bands of molecular weight 29-38, 48-54, 95-116, 121-162, >205 kDa, 80.8% for IgE to 29-38, 48-54, 95-121, > 205 kDa, and 65.4% for IgA to 29-38, 48-54, 81-93 kDa. Candidates for diagnostic markers should be IgG antibodies to bands of low molecular weight (29-38 and 48-54 kDa). One group of patients presented the same antibody reactivity to all bands throughout the follow-up study; in the other group, antibodies decayed partially or completely to some or all bands, but these changes were not correlated with time after chemotherapy. Candidates for monitoring patients after chemotherapy may be IgG antibodies to > 205 kDa fractions, IgA to 29-38, 48-54, 81-93 kDa and IgE to 95-121 kDa. Further identification of antigen epitopes related to these markers will allow the development of sensitive and specific immunoassays for the diagnosis and therapeutic assessment of toxocariasis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 53 2 61 65 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Toxocariasis Immunoblot assay Immunoglobulin isotypes Chemotherapy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Toxocariasis Immunoblot assay Immunoglobulin isotypes Chemotherapy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Guita Rubinsky-Elefant Sumie Hoshino-Shimizu Cristina Miuki Abe Jacob Maria Carmen Arroyo Sanchez Antonio Walter Ferreira Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis |
topic_facet |
Toxocariasis Immunoblot assay Immunoglobulin isotypes Chemotherapy Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
In human toxocariasis, there are few approaches using immunological markers for diagnosis and therapeutic assessment. An immunoblot (IB) assay using excretory-secretory Toxocara canis antigen was standardized for monitoring IgG, IgE and IgA antibodies in 27 children with toxocariasis (23 visceral, three mixed visceral and ocular, and one ocular form) for 22-116 months after chemotherapy. IB sensitivity was 100% for IgG antibodies to bands of molecular weight 29-38, 48-54, 95-116, 121-162, >205 kDa, 80.8% for IgE to 29-38, 48-54, 95-121, > 205 kDa, and 65.4% for IgA to 29-38, 48-54, 81-93 kDa. Candidates for diagnostic markers should be IgG antibodies to bands of low molecular weight (29-38 and 48-54 kDa). One group of patients presented the same antibody reactivity to all bands throughout the follow-up study; in the other group, antibodies decayed partially or completely to some or all bands, but these changes were not correlated with time after chemotherapy. Candidates for monitoring patients after chemotherapy may be IgG antibodies to > 205 kDa fractions, IgA to 29-38, 48-54, 81-93 kDa and IgE to 95-121 kDa. Further identification of antigen epitopes related to these markers will allow the development of sensitive and specific immunoassays for the diagnosis and therapeutic assessment of toxocariasis. |
format |
Article in Journal/Newspaper |
author |
Guita Rubinsky-Elefant Sumie Hoshino-Shimizu Cristina Miuki Abe Jacob Maria Carmen Arroyo Sanchez Antonio Walter Ferreira |
author_facet |
Guita Rubinsky-Elefant Sumie Hoshino-Shimizu Cristina Miuki Abe Jacob Maria Carmen Arroyo Sanchez Antonio Walter Ferreira |
author_sort |
Guita Rubinsky-Elefant |
title |
Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis |
title_short |
Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis |
title_full |
Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis |
title_fullStr |
Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis |
title_full_unstemmed |
Potential immunological markers for diagnosis and therapeutic assessment of toxocariasis |
title_sort |
potential immunological markers for diagnosis and therapeutic assessment of toxocariasis |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2011 |
url |
https://doi.org/10.1590/S0036-46652011000200001 https://doaj.org/article/071a6fbf272b4be9b8a7a31ccc15057f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 53, Iss 2, Pp 61-65 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652011000200001&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652011000200001 https://doaj.org/article/071a6fbf272b4be9b8a7a31ccc15057f |
op_doi |
https://doi.org/10.1590/S0036-46652011000200001 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
53 |
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2 |
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61 |
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65 |
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