Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em Lages, Estado de Santa Catarina, 2004-2005
INTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health inf...
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Sociedade Brasileira de Medicina Tropical (SBMT)
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ftdoajarticles:oai:doaj.org/article:06ce1d3632d144b09a862d0b4b74acda 2023-05-15T15:17:23+02:00 Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em Lages, Estado de Santa Catarina, 2004-2005 Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz 2011-06-01T00:00:00Z https://doaj.org/article/06ce1d3632d144b09a862d0b4b74acda EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300016 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/06ce1d3632d144b09a862d0b4b74acda Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 339-343 (2011) Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia Cysticercosis Immunodiagnosis Epidemiology Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles 2022-12-31T02:52:50Z INTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health information. Interviews and blood sample collections by finger prick on Whatman filter paper were performed from August 2004 to May 2005. Observation determined that 850 samples were suitable for analysis and were tested by ELISA using vesicular fluid of Taenia crassiceps heterologous antigen. To ensure the reliability of the results, 77 samples of the dried blood were matched with sera. The reactive samples were submitted to a serum confirmatory immunoblot (IB) test using purified Taenia crassiceps glycoproteins. RESULTS: The ELISA results for the dried blood and serum samples were statistically consistent. ELISA was positive in 186 (21.9%) out of 850 individuals. A group of 213 individuals were asked to collect vein blood for IB (186 with positive result in ELISA and 27 with inappropriate whole blood samples) and 130 attended the request. The IB was positive in 29 (3.4%) out of 850 individuals. A significant correlation (p = 0.0364) was determined among individuals who tested positive in the IB assay who practiced both pig rearing and kitchen gardening. CONCLUSIONS: ELISA with dried blood eluted from filter paper was suitable for cysticercosis population surveys. In Lages, human infection was associated with pig rearing and kitchen gardening. The prevalence index was compatible with other Latin American endemic areas. INTRODUÇÃO: O primeiro levantamento sobre cisticercose humana e identificação dos fatores de risco associados à transmissão, foram realizados em Lages, SC. MÉTODOS: Oitocentos e setenta e sete voluntários de regiões periurbana e rural foram entrevistados e forneceram informações demográficas e condições sanitárias e de saúde. Amostras de sangue foram coletadas por meio de punção digital em papel filtro ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia Cysticercosis Immunodiagnosis Epidemiology Arctic medicine. Tropical medicine RC955-962 |
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Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia Cysticercosis Immunodiagnosis Epidemiology Arctic medicine. Tropical medicine RC955-962 Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em Lages, Estado de Santa Catarina, 2004-2005 |
topic_facet |
Cisticercose Taenia crassiceps Imunodiagnóstico Epidemiologia Cysticercosis Immunodiagnosis Epidemiology Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Human serofrequency of antibodies against Taenia solium antigens was determined and risk factors for cysticercosis transmission were identified. METHODS: Individuals (n=878) from periurban and rural locations of Lages, SC, were interviewed to gather demographic, sanitary and health information. Interviews and blood sample collections by finger prick on Whatman filter paper were performed from August 2004 to May 2005. Observation determined that 850 samples were suitable for analysis and were tested by ELISA using vesicular fluid of Taenia crassiceps heterologous antigen. To ensure the reliability of the results, 77 samples of the dried blood were matched with sera. The reactive samples were submitted to a serum confirmatory immunoblot (IB) test using purified Taenia crassiceps glycoproteins. RESULTS: The ELISA results for the dried blood and serum samples were statistically consistent. ELISA was positive in 186 (21.9%) out of 850 individuals. A group of 213 individuals were asked to collect vein blood for IB (186 with positive result in ELISA and 27 with inappropriate whole blood samples) and 130 attended the request. The IB was positive in 29 (3.4%) out of 850 individuals. A significant correlation (p = 0.0364) was determined among individuals who tested positive in the IB assay who practiced both pig rearing and kitchen gardening. CONCLUSIONS: ELISA with dried blood eluted from filter paper was suitable for cysticercosis population surveys. In Lages, human infection was associated with pig rearing and kitchen gardening. The prevalence index was compatible with other Latin American endemic areas. INTRODUÇÃO: O primeiro levantamento sobre cisticercose humana e identificação dos fatores de risco associados à transmissão, foram realizados em Lages, SC. MÉTODOS: Oitocentos e setenta e sete voluntários de regiões periurbana e rural foram entrevistados e forneceram informações demográficas e condições sanitárias e de saúde. Amostras de sangue foram coletadas por meio de punção digital em papel filtro ... |
format |
Article in Journal/Newspaper |
author |
Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz |
author_facet |
Maria Márcia Imenes Ishida Marília Sirianni dos Santos Almeida Noeli Maria Espíndola Alberto Iha Diana Ana Pereira Jean Gabriel de Souza Theopi Rados Varvakis Adelaide José Vaz |
author_sort |
Maria Márcia Imenes Ishida |
title |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em Lages, Estado de Santa Catarina, 2004-2005 |
title_short |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em Lages, Estado de Santa Catarina, 2004-2005 |
title_full |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em Lages, Estado de Santa Catarina, 2004-2005 |
title_fullStr |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em Lages, Estado de Santa Catarina, 2004-2005 |
title_full_unstemmed |
Seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in Lages, State of Santa Catarina, Brazil, 2004-2005 Estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em Lages, Estado de Santa Catarina, 2004-2005 |
title_sort |
seroepidemiological study of human cysticercosis with blood samples collected on filter paper, in lages, state of santa catarina, brazil, 2004-2005 estudo soroepidemiológico da cisticercose humana com amostras de sangue total coletado em papel filtro, em lages, estado de santa catarina, 2004-2005 |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doaj.org/article/06ce1d3632d144b09a862d0b4b74acda |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 3, Pp 339-343 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000300016 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/06ce1d3632d144b09a862d0b4b74acda |
_version_ |
1766347633685692416 |