Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells
Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells. Methods: Methylene blue assay was used to determine the IC50 value of the extract. Western blotting assays were done to analyze the expression of HPV oncoproteins (HPV18 E6 and E7) and ap...
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ftdoajarticles:oai:doaj.org/article:069d05661a9943fc8591e031a0c1c6d6 2023-05-15T15:09:03+02:00 Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells Nurul Hidayah Wahab Nur Afina Mohd Din Yee Ying Lim Noor Izani Noor Jamil Nor Fazila Che Mat 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.262080 https://doaj.org/article/069d05661a9943fc8591e031a0c1c6d6 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=8;spage=339;epage=345;aulast=Wahab https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.262080 https://doaj.org/article/069d05661a9943fc8591e031a0c1c6d6 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 8, Pp 339-345 (2019) oroxylum indicum proapoptotic anti-proliferative hela cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.262080 2022-12-31T03:32:58Z Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells. Methods: Methylene blue assay was used to determine the IC50 value of the extract. Western blotting assays were done to analyze the expression of HPV oncoproteins (HPV18 E6 and E7) and apoptotic molecules (caspase-3 and caspase-8). Reverse transcriptase PCR assays were performed to determine genetic alteration of tumor suppressors p53 and pRb and apoptosis markers Fas and FasL. Enzyme-linked immunosorbent assay (ELISA) was done to determine the expression of cytokine levels (IL-6 and IL-12). Results: The determination of IC50 value indicated a higher anti-proliferative activity of the extract compared to cisplatin. After 24 hours of treatment, Western blot analysis showed that treated HeLa cells exhibited a significant down-regulation of HPV18 oncoproteins E6 and E7, and a significant induction of caspase-8 and caspase-3 activation level. Meanwhile, the mRNA expressions of p53, pRb, Fas and FasL were significantly upregulated in treated cells. Moreover, ELISA showed an increased IL-12 and decreased IL-6 production after Oroxylum indicum treatment. Conclusions: The methanol extract of Oroxylum indicum has an anti-proliferative activity and proapoptotic potential. It induces localized-immunity improvements by altering cytokine production in HPV-positive cervical cancer cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 8 339 |
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Directory of Open Access Journals: DOAJ Articles |
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oroxylum indicum proapoptotic anti-proliferative hela cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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oroxylum indicum proapoptotic anti-proliferative hela cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nurul Hidayah Wahab Nur Afina Mohd Din Yee Ying Lim Noor Izani Noor Jamil Nor Fazila Che Mat Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells |
topic_facet |
oroxylum indicum proapoptotic anti-proliferative hela cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To examine the proapoptotic properties of Oroxylum indicum methanol extract on cervical cancer cells. Methods: Methylene blue assay was used to determine the IC50 value of the extract. Western blotting assays were done to analyze the expression of HPV oncoproteins (HPV18 E6 and E7) and apoptotic molecules (caspase-3 and caspase-8). Reverse transcriptase PCR assays were performed to determine genetic alteration of tumor suppressors p53 and pRb and apoptosis markers Fas and FasL. Enzyme-linked immunosorbent assay (ELISA) was done to determine the expression of cytokine levels (IL-6 and IL-12). Results: The determination of IC50 value indicated a higher anti-proliferative activity of the extract compared to cisplatin. After 24 hours of treatment, Western blot analysis showed that treated HeLa cells exhibited a significant down-regulation of HPV18 oncoproteins E6 and E7, and a significant induction of caspase-8 and caspase-3 activation level. Meanwhile, the mRNA expressions of p53, pRb, Fas and FasL were significantly upregulated in treated cells. Moreover, ELISA showed an increased IL-12 and decreased IL-6 production after Oroxylum indicum treatment. Conclusions: The methanol extract of Oroxylum indicum has an anti-proliferative activity and proapoptotic potential. It induces localized-immunity improvements by altering cytokine production in HPV-positive cervical cancer cells. |
format |
Article in Journal/Newspaper |
author |
Nurul Hidayah Wahab Nur Afina Mohd Din Yee Ying Lim Noor Izani Noor Jamil Nor Fazila Che Mat |
author_facet |
Nurul Hidayah Wahab Nur Afina Mohd Din Yee Ying Lim Noor Izani Noor Jamil Nor Fazila Che Mat |
author_sort |
Nurul Hidayah Wahab |
title |
Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells |
title_short |
Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells |
title_full |
Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells |
title_fullStr |
Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells |
title_full_unstemmed |
Proapoptotic activities of Oroxylum indicum leave extract in HeLa cells |
title_sort |
proapoptotic activities of oroxylum indicum leave extract in hela cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.262080 https://doaj.org/article/069d05661a9943fc8591e031a0c1c6d6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 8, Pp 339-345 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=8;spage=339;epage=345;aulast=Wahab https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.262080 https://doaj.org/article/069d05661a9943fc8591e031a0c1c6d6 |
op_doi |
https://doi.org/10.4103/2221-1691.262080 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
8 |
container_start_page |
339 |
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1766340285556588544 |