Bacterial enteropathogens associated with diarrhea in a rural population of Haiti
John C Jackson, Anthony L Farone, Mary B Farone Biology Department, Middle Tennessee State University, Murfreesboro, Tennessee, USA Purpose: Diarrheal disease is one of the leading causes of morbidity in developing countries. To further understand the epidemiology of diarrheal disease among a rural...
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ftdoajarticles:oai:doaj.org/article:068a118e4c2a4bf898f68a684e5da111 2023-05-15T15:13:05+02:00 Bacterial enteropathogens associated with diarrhea in a rural population of Haiti Jackson JC Farone AL Farone MB 2011-09-01T00:00:00Z https://doaj.org/article/068a118e4c2a4bf898f68a684e5da111 EN eng Dove Medical Press http://www.dovepress.com/bacterial-enteropathogens-associated-with-diarrhea-in-a-rural-populati-a8350 https://doaj.org/toc/1179-7282 1179-7282 https://doaj.org/article/068a118e4c2a4bf898f68a684e5da111 Research and Reports in Tropical Medicine, Vol 2011, Iss default, Pp 129-133 (2011) Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles 2022-12-31T15:38:36Z John C Jackson, Anthony L Farone, Mary B Farone Biology Department, Middle Tennessee State University, Murfreesboro, Tennessee, USA Purpose: Diarrheal disease is one of the leading causes of morbidity in developing countries. To further understand the epidemiology of diarrheal disease among a rural population surrounding Robillard, Haiti, fecal swabs from patients with diarrhea were screened for the presence of enteropathogenic bacteria. Patients and methods: Fecal swabs were collected from 34 patients with signs and symptoms of diarrhea and stored in BBLTM Cary-Blair transport medium (Becton, Dickinson and Company, Sparks, MD) until transit to the USA. Swab material was inoculated on to different enrichment and selective agars for incubation. Fermenting and nonfermenting bacteria that grew on the enteric selection media were identified by the BBLTM CrystalTM Enteric/Nonferementing Identification system (Becton, Dickinson and Company). Organisms identified as Escherichia coli were further screened for the presence of virulence factors by polymerase chain reaction (PCR). Results: Of 34 patients, no Campylobacter, Shigella, Salmonella, or Vibrio spp. were isolated from swabs transported to the USA for culture. Of 73 E. coli isolates cultured from the swabs, one enteropathogenic strain of E. coli was identified by multiplex PCR. Escherichia fergusonii and Cronobacter sakazakii, both potential gastrointestinal pathogens, were also isolated from patient stools. Conclusion: This study was undertaken to determine if bacterial enteropathogens could be detected in the stools of patients suffering from diarrhea or dysentery and, in the absence of sufficient facilities, rectal swabs could be transported to the USA for culture. Although several genera of overt enteropathogens were not detected, one enteropathogenic E. coli and other pathogenic enterobacteriaceae were successfully cultured and identified. Keywords: Escherichia, Cronobacter, diarrheagenic, stool Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Blair ENVELOPE(160.817,160.817,-72.533,-72.533) Robillard ENVELOPE(-65.733,-65.733,-68.317,-68.317) |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Jackson JC Farone AL Farone MB Bacterial enteropathogens associated with diarrhea in a rural population of Haiti |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
John C Jackson, Anthony L Farone, Mary B Farone Biology Department, Middle Tennessee State University, Murfreesboro, Tennessee, USA Purpose: Diarrheal disease is one of the leading causes of morbidity in developing countries. To further understand the epidemiology of diarrheal disease among a rural population surrounding Robillard, Haiti, fecal swabs from patients with diarrhea were screened for the presence of enteropathogenic bacteria. Patients and methods: Fecal swabs were collected from 34 patients with signs and symptoms of diarrhea and stored in BBLTM Cary-Blair transport medium (Becton, Dickinson and Company, Sparks, MD) until transit to the USA. Swab material was inoculated on to different enrichment and selective agars for incubation. Fermenting and nonfermenting bacteria that grew on the enteric selection media were identified by the BBLTM CrystalTM Enteric/Nonferementing Identification system (Becton, Dickinson and Company). Organisms identified as Escherichia coli were further screened for the presence of virulence factors by polymerase chain reaction (PCR). Results: Of 34 patients, no Campylobacter, Shigella, Salmonella, or Vibrio spp. were isolated from swabs transported to the USA for culture. Of 73 E. coli isolates cultured from the swabs, one enteropathogenic strain of E. coli was identified by multiplex PCR. Escherichia fergusonii and Cronobacter sakazakii, both potential gastrointestinal pathogens, were also isolated from patient stools. Conclusion: This study was undertaken to determine if bacterial enteropathogens could be detected in the stools of patients suffering from diarrhea or dysentery and, in the absence of sufficient facilities, rectal swabs could be transported to the USA for culture. Although several genera of overt enteropathogens were not detected, one enteropathogenic E. coli and other pathogenic enterobacteriaceae were successfully cultured and identified. Keywords: Escherichia, Cronobacter, diarrheagenic, stool |
format |
Article in Journal/Newspaper |
author |
Jackson JC Farone AL Farone MB |
author_facet |
Jackson JC Farone AL Farone MB |
author_sort |
Jackson JC |
title |
Bacterial enteropathogens associated with diarrhea in a rural population of Haiti |
title_short |
Bacterial enteropathogens associated with diarrhea in a rural population of Haiti |
title_full |
Bacterial enteropathogens associated with diarrhea in a rural population of Haiti |
title_fullStr |
Bacterial enteropathogens associated with diarrhea in a rural population of Haiti |
title_full_unstemmed |
Bacterial enteropathogens associated with diarrhea in a rural population of Haiti |
title_sort |
bacterial enteropathogens associated with diarrhea in a rural population of haiti |
publisher |
Dove Medical Press |
publishDate |
2011 |
url |
https://doaj.org/article/068a118e4c2a4bf898f68a684e5da111 |
long_lat |
ENVELOPE(160.817,160.817,-72.533,-72.533) ENVELOPE(-65.733,-65.733,-68.317,-68.317) |
geographic |
Arctic Blair Robillard |
geographic_facet |
Arctic Blair Robillard |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Research and Reports in Tropical Medicine, Vol 2011, Iss default, Pp 129-133 (2011) |
op_relation |
http://www.dovepress.com/bacterial-enteropathogens-associated-with-diarrhea-in-a-rural-populati-a8350 https://doaj.org/toc/1179-7282 1179-7282 https://doaj.org/article/068a118e4c2a4bf898f68a684e5da111 |
_version_ |
1766343684206362624 |