A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases.
BACKGROUND: Food-borne helminthiases (FBHs) have become increasingly important due to frequent occurrence and worldwide distribution. There is increasing demand for developing more sensitive, high-throughput techniques for the simultaneous detection of multiple parasitic diseases due to limitations...
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ftdoajarticles:oai:doaj.org/article:0689cec3baf94f1192bdae76b39c7aea 2023-05-15T15:12:57+02:00 A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases. Jia-Xu Chen Mu-Xin Chen Lin Ai Jun-Hu Chen Shao-Hong Chen Yong-Nian Zhang Yu-Chun Cai Xing-Quan Zhu Xiao-Nong Zhou 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001899 https://doaj.org/article/0689cec3baf94f1192bdae76b39c7aea EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3510079?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001899 https://doaj.org/article/0689cec3baf94f1192bdae76b39c7aea PLoS Neglected Tropical Diseases, Vol 6, Iss 11, p e1899 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001899 2022-12-31T04:46:36Z BACKGROUND: Food-borne helminthiases (FBHs) have become increasingly important due to frequent occurrence and worldwide distribution. There is increasing demand for developing more sensitive, high-throughput techniques for the simultaneous detection of multiple parasitic diseases due to limitations in differential clinical diagnosis of FBHs with similar symptoms. These infections are difficult to diagnose correctly by conventional diagnostic approaches including serological approaches. METHODOLOGY/PRINCIPAL FINDINGS: In this study, antigens obtained from 5 parasite species, namely Cysticercus cellulosae, Angiostrongylus cantonensis, Paragonimus westermani, Trichinella spiralis and Spirometra sp., were semi-purified after immunoblotting. Sera from 365 human cases of helminthiasis and 80 healthy individuals were assayed with semi-purified antigens by both a protein microarray and the enzyme-linked immunosorbent assay (ELISA). The sensitivity, specificity and simplicity of each test for the end-user were evaluated. The specificity of the tests ranged from 97.0% (95% confidence interval (CI): 95.3-98.7%) to 100.0% (95% CI: 100.0%) in the protein microarray and from 97.7% (95% CI: 96.2-99.2%) to 100.0% (95% CI: 100.0%) in ELISA. The sensitivity varied from 85.7% (95% CI: 75.1-96.3%) to 92.1% (95% CI: 83.5-100.0%) in the protein microarray, while the corresponding values for ELISA were 82.0% (95% CI: 71.4-92.6%) to 92.1% (95% CI: 83.5-100.0%). Furthermore, the Youden index spanned from 0.83 to 0.92 in the protein microarray and from 0.80 to 0.92 in ELISA. For each parasite, the Youden index from the protein microarray was often slightly higher than the one from ELISA even though the same antigen was used. CONCLUSIONS/SIGNIFICANCE: The protein microarray platform is a convenient, versatile, high-throughput method that can easily be adapted to massive FBH screening. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 11 e1899 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jia-Xu Chen Mu-Xin Chen Lin Ai Jun-Hu Chen Shao-Hong Chen Yong-Nian Zhang Yu-Chun Cai Xing-Quan Zhu Xiao-Nong Zhou A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Food-borne helminthiases (FBHs) have become increasingly important due to frequent occurrence and worldwide distribution. There is increasing demand for developing more sensitive, high-throughput techniques for the simultaneous detection of multiple parasitic diseases due to limitations in differential clinical diagnosis of FBHs with similar symptoms. These infections are difficult to diagnose correctly by conventional diagnostic approaches including serological approaches. METHODOLOGY/PRINCIPAL FINDINGS: In this study, antigens obtained from 5 parasite species, namely Cysticercus cellulosae, Angiostrongylus cantonensis, Paragonimus westermani, Trichinella spiralis and Spirometra sp., were semi-purified after immunoblotting. Sera from 365 human cases of helminthiasis and 80 healthy individuals were assayed with semi-purified antigens by both a protein microarray and the enzyme-linked immunosorbent assay (ELISA). The sensitivity, specificity and simplicity of each test for the end-user were evaluated. The specificity of the tests ranged from 97.0% (95% confidence interval (CI): 95.3-98.7%) to 100.0% (95% CI: 100.0%) in the protein microarray and from 97.7% (95% CI: 96.2-99.2%) to 100.0% (95% CI: 100.0%) in ELISA. The sensitivity varied from 85.7% (95% CI: 75.1-96.3%) to 92.1% (95% CI: 83.5-100.0%) in the protein microarray, while the corresponding values for ELISA were 82.0% (95% CI: 71.4-92.6%) to 92.1% (95% CI: 83.5-100.0%). Furthermore, the Youden index spanned from 0.83 to 0.92 in the protein microarray and from 0.80 to 0.92 in ELISA. For each parasite, the Youden index from the protein microarray was often slightly higher than the one from ELISA even though the same antigen was used. CONCLUSIONS/SIGNIFICANCE: The protein microarray platform is a convenient, versatile, high-throughput method that can easily be adapted to massive FBH screening. |
format |
Article in Journal/Newspaper |
author |
Jia-Xu Chen Mu-Xin Chen Lin Ai Jun-Hu Chen Shao-Hong Chen Yong-Nian Zhang Yu-Chun Cai Xing-Quan Zhu Xiao-Nong Zhou |
author_facet |
Jia-Xu Chen Mu-Xin Chen Lin Ai Jun-Hu Chen Shao-Hong Chen Yong-Nian Zhang Yu-Chun Cai Xing-Quan Zhu Xiao-Nong Zhou |
author_sort |
Jia-Xu Chen |
title |
A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases. |
title_short |
A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases. |
title_full |
A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases. |
title_fullStr |
A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases. |
title_full_unstemmed |
A protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases. |
title_sort |
protein microarray for the rapid screening of patients suspected of infection with various food-borne helminthiases. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001899 https://doaj.org/article/0689cec3baf94f1192bdae76b39c7aea |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 11, p e1899 (2012) |
op_relation |
http://europepmc.org/articles/PMC3510079?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001899 https://doaj.org/article/0689cec3baf94f1192bdae76b39c7aea |
op_doi |
https://doi.org/10.1371/journal.pntd.0001899 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
11 |
container_start_page |
e1899 |
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1766343558718029824 |