The impact of autophagy on arbovirus infection of mosquito cells.
Macroautophagy is an evolutionarily conserved cellular process critical for maintaining cellular homeostasis. It can additionally function as an innate immune response to viral infection as has been demonstrated for a number of arthropod-borne (arbo-) viruses. Arboviruses are maintained in a transmi...
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ftdoajarticles:oai:doaj.org/article:06868a12cb3d406eb40d5b9b3ce03667 2023-05-15T15:10:03+02:00 The impact of autophagy on arbovirus infection of mosquito cells. Doug E Brackney Maria A Correa Duncan W Cozens 2020-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007754 https://doaj.org/article/06868a12cb3d406eb40d5b9b3ce03667 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007754 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007754 https://doaj.org/article/06868a12cb3d406eb40d5b9b3ce03667 PLoS Neglected Tropical Diseases, Vol 14, Iss 5, p e0007754 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007754 2022-12-31T07:48:36Z Macroautophagy is an evolutionarily conserved cellular process critical for maintaining cellular homeostasis. It can additionally function as an innate immune response to viral infection as has been demonstrated for a number of arthropod-borne (arbo-) viruses. Arboviruses are maintained in a transmission cycle between vertebrate hosts and invertebrate vectors yet the majority of studies assessing autophagy-arbovirus interactions have been limited to the mammalian host. Therefore we evaluated the role of autophagy during arbovirus infection of the invertebrate vector using the tractable Aag2 Aedes aegypti mosquito cell culture system. Our data demonstrates that autophagy is significantly induced in mosquito cells upon infection with two divergent arboviruses: dengue virus-2 (DENV-2; Flaviviridae, Flavivirus) and chikungunya virus (CHIKV; Togaviridae, Alphavirus). While assessing the role of autophagy during arbovirus infection, we observed a somewhat paradoxical outcome. Both induction and suppression of autophagy via torin-1 and spautin-1, respectively, resulted in increased viral titers for both viruses, yet suppression of autophagy-related genes had no effect. Interestingly, chemical modulators of autophagy had either no effect or opposite effects in another widely used mosquito cell line, C6/36 Aedes albopictus cells. Together, our data reveals a limited role for autophagy during arbovirus infection of mosquito cells. Further, our findings suggest that commonly used chemical modulators of autophagy alter mosquito cells in such a way as to promote viral replication; however, it is unclear if this occurs directly through autophagic manipulation or other means. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 5 e0007754 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Doug E Brackney Maria A Correa Duncan W Cozens The impact of autophagy on arbovirus infection of mosquito cells. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Macroautophagy is an evolutionarily conserved cellular process critical for maintaining cellular homeostasis. It can additionally function as an innate immune response to viral infection as has been demonstrated for a number of arthropod-borne (arbo-) viruses. Arboviruses are maintained in a transmission cycle between vertebrate hosts and invertebrate vectors yet the majority of studies assessing autophagy-arbovirus interactions have been limited to the mammalian host. Therefore we evaluated the role of autophagy during arbovirus infection of the invertebrate vector using the tractable Aag2 Aedes aegypti mosquito cell culture system. Our data demonstrates that autophagy is significantly induced in mosquito cells upon infection with two divergent arboviruses: dengue virus-2 (DENV-2; Flaviviridae, Flavivirus) and chikungunya virus (CHIKV; Togaviridae, Alphavirus). While assessing the role of autophagy during arbovirus infection, we observed a somewhat paradoxical outcome. Both induction and suppression of autophagy via torin-1 and spautin-1, respectively, resulted in increased viral titers for both viruses, yet suppression of autophagy-related genes had no effect. Interestingly, chemical modulators of autophagy had either no effect or opposite effects in another widely used mosquito cell line, C6/36 Aedes albopictus cells. Together, our data reveals a limited role for autophagy during arbovirus infection of mosquito cells. Further, our findings suggest that commonly used chemical modulators of autophagy alter mosquito cells in such a way as to promote viral replication; however, it is unclear if this occurs directly through autophagic manipulation or other means. |
format |
Article in Journal/Newspaper |
author |
Doug E Brackney Maria A Correa Duncan W Cozens |
author_facet |
Doug E Brackney Maria A Correa Duncan W Cozens |
author_sort |
Doug E Brackney |
title |
The impact of autophagy on arbovirus infection of mosquito cells. |
title_short |
The impact of autophagy on arbovirus infection of mosquito cells. |
title_full |
The impact of autophagy on arbovirus infection of mosquito cells. |
title_fullStr |
The impact of autophagy on arbovirus infection of mosquito cells. |
title_full_unstemmed |
The impact of autophagy on arbovirus infection of mosquito cells. |
title_sort |
impact of autophagy on arbovirus infection of mosquito cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0007754 https://doaj.org/article/06868a12cb3d406eb40d5b9b3ce03667 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 5, p e0007754 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007754 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007754 https://doaj.org/article/06868a12cb3d406eb40d5b9b3ce03667 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007754 |
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PLOS Neglected Tropical Diseases |
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14 |
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5 |
container_start_page |
e0007754 |
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