Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves
Objective: To evaluate acute oral toxicity and anti-arthritic activity of the methanolic extract of Convolvulus arvensis L. leaves. Methods: Safety was assessed by acute oral toxicity (OECD 425) study. Anti-arthritic activity was explored by in vitro (inhibition of protein denaturation) and in vivo...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:067b22e353b048c080df2d973ea3a104 2023-05-15T15:07:31+02:00 Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves Uzma Saleem Shingraf Zaib Sana Khalid Fareeha Anwar Muhammad Furqan Akhtar Bashir Ahmad 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.290869 https://doaj.org/article/067b22e353b048c080df2d973ea3a104 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=10;spage=442;epage=451;aulast=Saleem https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.290869 https://doaj.org/article/067b22e353b048c080df2d973ea3a104 Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 10, Pp 442-451 (2020) convolvulus arvensis arthritis phytoconstituents molecular docking Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.290869 2022-12-31T13:47:01Z Objective: To evaluate acute oral toxicity and anti-arthritic activity of the methanolic extract of Convolvulus arvensis L. leaves. Methods: Safety was assessed by acute oral toxicity (OECD 425) study. Anti-arthritic activity was explored by in vitro (inhibition of protein denaturation) and in vivo (Complete Freund’s adjuvant-induced arthritis and carrageenan-induced inflammation) methods. Antioxidant potential was determined by assessing ferric reducing power, DPPH inhibition, and H2O2 scavenging assays. Furthermore, molecular docking was done to check interactions between the plant constituents and cyclooxygenases (COX-1 and COX-2). Quercetin, gallic acid, caffeic acid, syringic acid, sinapic acid, and vanillic acid were quantified by HPLC and eight compounds were identified by GC-MS analysis. Results: No mortality and abnormality in biochemical parameters were observed in the toxicity study. Histological analysis of vital organs also supported these biochemical results. The in vitro and in vivo studies showed that the methanolic extract of leaves of Convolvulus arvensis exhibited dose-dependent anti-arthritic and anti-oxidant potential. Molecular docking showed better interactions of plant compounds with cyclooxygenases as compared to standard ibuprofen. Conclusions: Convolvulus arvensis exhibits strong anti-arthritic activity, justifying the traditional use of the herbal drug. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 10 442 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
convolvulus arvensis arthritis phytoconstituents molecular docking Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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convolvulus arvensis arthritis phytoconstituents molecular docking Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Uzma Saleem Shingraf Zaib Sana Khalid Fareeha Anwar Muhammad Furqan Akhtar Bashir Ahmad Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves |
topic_facet |
convolvulus arvensis arthritis phytoconstituents molecular docking Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate acute oral toxicity and anti-arthritic activity of the methanolic extract of Convolvulus arvensis L. leaves. Methods: Safety was assessed by acute oral toxicity (OECD 425) study. Anti-arthritic activity was explored by in vitro (inhibition of protein denaturation) and in vivo (Complete Freund’s adjuvant-induced arthritis and carrageenan-induced inflammation) methods. Antioxidant potential was determined by assessing ferric reducing power, DPPH inhibition, and H2O2 scavenging assays. Furthermore, molecular docking was done to check interactions between the plant constituents and cyclooxygenases (COX-1 and COX-2). Quercetin, gallic acid, caffeic acid, syringic acid, sinapic acid, and vanillic acid were quantified by HPLC and eight compounds were identified by GC-MS analysis. Results: No mortality and abnormality in biochemical parameters were observed in the toxicity study. Histological analysis of vital organs also supported these biochemical results. The in vitro and in vivo studies showed that the methanolic extract of leaves of Convolvulus arvensis exhibited dose-dependent anti-arthritic and anti-oxidant potential. Molecular docking showed better interactions of plant compounds with cyclooxygenases as compared to standard ibuprofen. Conclusions: Convolvulus arvensis exhibits strong anti-arthritic activity, justifying the traditional use of the herbal drug. |
format |
Article in Journal/Newspaper |
author |
Uzma Saleem Shingraf Zaib Sana Khalid Fareeha Anwar Muhammad Furqan Akhtar Bashir Ahmad |
author_facet |
Uzma Saleem Shingraf Zaib Sana Khalid Fareeha Anwar Muhammad Furqan Akhtar Bashir Ahmad |
author_sort |
Uzma Saleem |
title |
Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves |
title_short |
Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves |
title_full |
Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves |
title_fullStr |
Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves |
title_full_unstemmed |
Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves |
title_sort |
chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of convolvulus arvensis l. leaves |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.290869 https://doaj.org/article/067b22e353b048c080df2d973ea3a104 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 10, Pp 442-451 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=10;spage=442;epage=451;aulast=Saleem https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.290869 https://doaj.org/article/067b22e353b048c080df2d973ea3a104 |
op_doi |
https://doi.org/10.4103/2221-1691.290869 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
10 |
container_issue |
10 |
container_start_page |
442 |
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1766339006056890368 |