Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance?
BACKGROUND:During infection, successful bacterial clearance is achieved via the host immune system acting in conjunction with appropriate antibiotic therapy. However, it still remains a tip of the iceberg as to where persistent pathogens namely, Burkholderia pseudomallei (B. pseudomallei) reside/hid...
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ftdoajarticles:oai:doaj.org/article:06484212ecf548208107e3b8097f542c 2023-05-15T15:08:09+02:00 Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance? Jamuna Vadivelu Kumutha Malar Vellasamy Jaikumar Thimma Vanitha Mariappan Wen-Tyng Kang Leang-Chung Choh Esaki M Shankar Kum Thong Wong 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005241 https://doaj.org/article/06484212ecf548208107e3b8097f542c EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5234843?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005241 https://doaj.org/article/06484212ecf548208107e3b8097f542c PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005241 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005241 2022-12-31T01:32:54Z BACKGROUND:During infection, successful bacterial clearance is achieved via the host immune system acting in conjunction with appropriate antibiotic therapy. However, it still remains a tip of the iceberg as to where persistent pathogens namely, Burkholderia pseudomallei (B. pseudomallei) reside/hide to escape from host immune sensors and antimicrobial pressure. METHODS:We used transmission electron microscopy (TEM) to investigate post-mortem tissue sections of patients with clinical melioidosis to identify the localisation of a recently identified gut microbiome, B. pseudomallei within host cells. The intranuclear presence of B. pseudomallei was confirmed using transmission electron microscopy (TEM) of experimentally infected guinea pig spleen tissues and Live Z-stack, and ImageJ analysis of fluorescence microscopy analysis of in vitro infection of A549 human lung epithelial cells. RESULTS:TEM investigations revealed intranuclear localization of B. pseudomallei in cells of infected human lung and guinea pig spleen tissues. We also found that B. pseudomallei induced actin polymerization following infection of A549 human lung epithelial cells. Infected A549 lung epithelial cells using 3D-Laser scanning confocal microscopy (LSCM) and immunofluorescence microscopy confirmed the intranuclear localization of B. pseudomallei. CONCLUSION:B. pseudomallei was found within the nuclear compartment of host cells. The nucleus may play a role as an occult or transient niche for persistence of intracellular pathogens, potentially leading to recurrrent episodes or recrudescence of infection. Article in Journal/Newspaper Arctic Iceberg* Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 1 e0005241 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jamuna Vadivelu Kumutha Malar Vellasamy Jaikumar Thimma Vanitha Mariappan Wen-Tyng Kang Leang-Chung Choh Esaki M Shankar Kum Thong Wong Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance? |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:During infection, successful bacterial clearance is achieved via the host immune system acting in conjunction with appropriate antibiotic therapy. However, it still remains a tip of the iceberg as to where persistent pathogens namely, Burkholderia pseudomallei (B. pseudomallei) reside/hide to escape from host immune sensors and antimicrobial pressure. METHODS:We used transmission electron microscopy (TEM) to investigate post-mortem tissue sections of patients with clinical melioidosis to identify the localisation of a recently identified gut microbiome, B. pseudomallei within host cells. The intranuclear presence of B. pseudomallei was confirmed using transmission electron microscopy (TEM) of experimentally infected guinea pig spleen tissues and Live Z-stack, and ImageJ analysis of fluorescence microscopy analysis of in vitro infection of A549 human lung epithelial cells. RESULTS:TEM investigations revealed intranuclear localization of B. pseudomallei in cells of infected human lung and guinea pig spleen tissues. We also found that B. pseudomallei induced actin polymerization following infection of A549 human lung epithelial cells. Infected A549 lung epithelial cells using 3D-Laser scanning confocal microscopy (LSCM) and immunofluorescence microscopy confirmed the intranuclear localization of B. pseudomallei. CONCLUSION:B. pseudomallei was found within the nuclear compartment of host cells. The nucleus may play a role as an occult or transient niche for persistence of intracellular pathogens, potentially leading to recurrrent episodes or recrudescence of infection. |
format |
Article in Journal/Newspaper |
author |
Jamuna Vadivelu Kumutha Malar Vellasamy Jaikumar Thimma Vanitha Mariappan Wen-Tyng Kang Leang-Chung Choh Esaki M Shankar Kum Thong Wong |
author_facet |
Jamuna Vadivelu Kumutha Malar Vellasamy Jaikumar Thimma Vanitha Mariappan Wen-Tyng Kang Leang-Chung Choh Esaki M Shankar Kum Thong Wong |
author_sort |
Jamuna Vadivelu |
title |
Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance? |
title_short |
Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance? |
title_full |
Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance? |
title_fullStr |
Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance? |
title_full_unstemmed |
Survival and Intra-Nuclear Trafficking of Burkholderia pseudomallei: Strategies of Evasion from Immune Surveillance? |
title_sort |
survival and intra-nuclear trafficking of burkholderia pseudomallei: strategies of evasion from immune surveillance? |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005241 https://doaj.org/article/06484212ecf548208107e3b8097f542c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Iceberg* |
genre_facet |
Arctic Iceberg* |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005241 (2017) |
op_relation |
http://europepmc.org/articles/PMC5234843?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005241 https://doaj.org/article/06484212ecf548208107e3b8097f542c |
op_doi |
https://doi.org/10.1371/journal.pntd.0005241 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
1 |
container_start_page |
e0005241 |
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1766339566630862848 |